酵母双杂交技术及应用的研究进展

›› 2013, Vol. 40 ›› Issue (9): 105-108.

酵母双杂交技术及应用的研究进展

郑立双¹, 李向楠², 孙城涛², 刘红羽², 刘勋², 贺明², 吕文发²

1. 吉林农业大学生命科学学院, 吉林长春 130118;
2. 吉林农业大学动物科学技术学院, 吉林长春 130118

收稿日期:2013-01-28 出版日期:2013-09-20 发布日期:2013-09-18
通讯作者: 吕文发 E-mail:wenfa2000@yahoo.com.cn
作者简介:郑立双(1985-),男,吉林人,硕士生,研究方向:生物大分子的结构与功能。
基金资助:
国家自然科学基金(30972108);吉林省政府人才开发基金。

Research Progress on Yeast Two-hybrid Technology and Application

ZHENG Li-shuang¹, LI Xiang-nan², SUN Cheng-tao², LIU Hong-yu², LIU Xun², HE Ming², LV Wen-fa²

1. College of Life Sciences, Jilin Agricultural University, Changchun 130118, China;
2. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China

Received:2013-01-28 Online:2013-09-20 Published:2013-09-18

摘要/Abstract

摘要： 酵母双杂交技术是研究蛋白质与蛋白质相互作用的一种非常有效的分子生物学方法,它是一种能直接检测胞内蛋白质相互作用较为简便、快捷、高效、敏感、广泛的方法。作者将主要介绍酵母双杂交技术的基本原理、应用及优缺点,现就酵母双杂交技术及其应用的研究进展作一综述。

关键词: 酵母双杂交技术; 真核细胞; 蛋白质

Abstract: Yeast two-hybrid technology is an effective molecular biology method which is used to study the interaction of proteins. It is relatively simple, fast, efficient, sensitive, extensive and can directly detect the interaction of intracellular proteins. This paper mainly introduces the basic principle, applications, advantages and disadvantages of yeast two-hybrid technology, now the research on yeast two-hybrid system technology and application will be reviewed.

Key words: yeast two-hybrid system; eukaryotic cell; protein

中图分类号:

郑立双, 李向楠, 孙城涛, 刘红羽, 刘勋, 贺明, 吕文发. 酵母双杂交技术及应用的研究进展[J]. , 2013, 40(9): 105-108.

ZHENG Li-shuang, LI Xiang-nan, SUN Cheng-tao, LIU Hong-yu, LIU Xun, HE Ming, LV Wen-fa. Research Progress on Yeast Two-hybrid Technology and Application[J]. , 2013, 40(9): 105-108.

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