家畜体内防御素多态性和表达的研究进展

摘要/Abstract

摘要： 抗菌肽可抵御细菌、病毒和真菌的入侵,在人类和家畜的先天性防御系统中具有重要的作用,是有望用于预防和治疗的药物,因此,抗菌肽被称为"新一代的抗生素"。抗菌肽是一个阳离子肽,其中的一个大家族就是防御素。防御素的结构中普通带有一个β-片状结构,其中包含3个分子内二硫键,可分为α-防御素、β-防御素和θ-防御素,其中β-防御素是最大的一个家族,在人类和家畜的组织内均有表达。结合已有的研究成果,作者主要对家畜体内防御素的表达、多态性及其作为健康和生产性能的遗传标记的潜在应用作一综述。

关键词: 家畜; 防御素; 表达; 多态性

Abstract: Antimicrobial peptides have important effects on the innate resistance system of human and livestock due to their activity against bacteria, viruses and fungi. They also are expected to be used as preventive and therapeutic medicine. Therefore they are called 'new generation antibiotics'. Antimicrobial peptides are cationic peptides, and the most numerous group of them is a family of defensins. Among them the most common are peptides with a beta-sheet structure containing three intra-molecular disulphide bonds, called defensins, comprising three classes: alpha-, beta- and theta-defensin. The family of beta-defensins is the largest one, and their transcripts have been found in many tissues of human and livestock. The aim of this paper is to present the current knowledge about antimicrobial peptides from the defensins family in farm animals, their expression, polymorphism, as well as the potential of their use as genetic markers of health and production traits.

Key words: livestock; defensins; expression; polymorphism

中图分类号:

S852.4

李砚, 杨银凤. 家畜体内防御素多态性和表达的研究进展[J]. , 2013, 40(3): 160-168.

LI Yan, YANG Yin-feng. Research Progress of Polymorphism and Expression of Defensins in vivo of Livestock[J]. , 2013, 40(3): 160-168.

参考文献

1 Anbu K A, More T, Kumar A.Isolation and characterisation of cationic antibacterial proteins and peptides from goat tongue epithelium[J].Indian J Anim Sci, 2003, 73: 1307～1311.
2 Aono S, Li C h, Zhang G, et al.Molecular and functional characterization of bovine beta-defensin-1[J].Vet Immunol Immunopathol, 2006, 113(1～2): 181～190.
3 Bagnicka E, Flisikowski K, Strzakowska N, et al.Expression level of goat beta-defensin genes in different goat tissues and in somatic cells of goat milk preliminary study[J]. Proceedings of the XI Baltic Animal Breeding and Genetics Conference.Palanga Lithuania, 2005.
4 Bagnicka E, Strzakowska N, Flisikowski K, et al.The polymorphism in the beta4-defensin gene and its assotiation with production and somatic cell count in Holstein-Friesian cows[J].J Anim Breed Genet, 2007, 124(3):150～156.
5 Bagnicka E, Strzakowska N, Szreder T, et al.A/C polymorphism in the β-4 defensin gene and its association with phenotypic and breeding values of milk production traits in Polish-Friesian cows[J].Anim Sci Pap Rep, 2008, 26(4):239～250.
6 Brogden K A, Ackermann M, McCray Jr P B, et al.Antimicrobial peptides in animals and their role in host defence[J].Int J Antimicrob Agents, 2003, 22(5): 465～478.
7 Brogden K A.Antimicrobial peptides: Pore formation or metabolic inhibitors in bacteria?[J].Nat Rev Microbiol, 2005, 3(3): 238～250.
8 Bruhn O, Regenhard P, Michalek M, et al.A novel horse alpha-defensin: Gene transcription, recombinant expression and characterization of the structure and function[J].Biochem J, 2007, 407(2):267～276.
9 Bruhn O, Cauchard J, Schlusselhuber M, et al.Antimicrobial properties of the equine alpha-defensin DEFA1 against bacterial horse pathogens[J].Vet Immunol Immunopathol, 2009a, 130(1～2):102～106.
10 Bruhn O, Paul S, Tetens J, et al.The repertoire of equine intestinal alpha-defensins[J].BMC Genomics, 2009b,10:631～644.
11 Chen J, Qi S, Guo R, et al.Different messenger RNA expression for the antimicrobial peptides beta-defensins between Meishan and crossbred pigs[J].Mol Biol Rep, 2010, 37(3): 1633～1639.
12 Cormican P, Meade K G, Cahalane S, et al.Evolution, expression and effectivness in a cluster of novel bovine beta-defensins[J].Immunogenet, 2008,60(3～4):147～156.
13 Das D K, Sharma B, Mitra A, et al.Molecular cloning and characterization of beta-defensin cDNA expressed in distal ileum of buffalo (Bubalus bubalis)[J].DNA Seq, 2005, 16(1): 16～20.
14 Davies D, Meade K G, Herath S, et al.Toll-like receptor and antimicrobial peptide expression in the bovine endometrium[J]. Reprod Biol Endocrinol, 2008, 6:53.
15 Davis E G, Sang Y, Blecha F.Equine beta-defensin-1: Full-length cDNA sequence and tissue expression[J].Vet Immunol Immunopath, 2004, 99(1～2):127～132.
16 Diamond G, Zasloff M, Eck H, et al.Tracheal antimicrbial peptide, a novel cysteine-rich peptide from mammalian tracheal mucosa: Peptide isolation and cloning of a cDNA[J].Proc Natl Acad Sci USA, 1991, 88(9):3952～3956.
17 Diamond G, Jones D E, Bevins C J.Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene[J].Proc Natl Acad Sci USA, 1993, 90(10):4596～4600.
18 Diamond G, Kaiser V, Rhodes J, et al.Transcriptional regulation of beta-defensin gene expression in tracheal epithelial cells[J].Infect Immun, 2000, 68(11):113～119.
19 Galindo R C, Ayoubi P, Naranjo V, et al.Gene expression profiles of European wild boar naturally infected with Mycobacterium bovis[J].Vet Immunol Immunopathol, 2009, 129(1～2):119～125.
20 Ganz T.Defensins: Antimicrobial peptides of vertebrates[J].C R Biologies, 2004, 32:539～549.
21 Gordon Y J, Romanowski E G.A review of antimicrobial peptides and their therapeutic potential as anti-infective drugs[J].Curr Eye Res, 2005, 30(7):505～515.
22 Huttner K M, Bresinski-Caliguri D J, Mahoney M M, et al.Antimicrobial peptide expression is developmentally regulated in the ovine gastrointenstinal tract[J].J Nutr, 1998a, 128(2 suppl):297～299.
23 Huttner K M, Lambeth M R, Burkin H R, et al.Localization and genomic organization of sheep antimicrobial peptide gene[J].Gene, 1998b, 206(1):85～91.
24 Iannuzzi L, Gallagher D S, Di Meo G P, et al.High-resolution FISH mapping of beta-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypesz[J].Cytogenet Cell Genet, 1996,75(1):10～13.
25 Kamysz W, Okrój M, ukasiak J.Novel properties of antimicrobial peptides[J].Acta Biochim Pol, 2003, 50:461～469.
26 Lehrer R I, Ganz T.Antimicrobial peptides in mammalian and insect host defence[J].Curr Opin Immunol, 1999,11(1):23～27.
27 Liang Q L, Zhou K, He H X.Retrocyclin 2: A new therapy against avian influenza H5N1 virus in vivo and in vitro[J].Biotechnol Lett, 2010,32(3):387～392.
28 Looft C, Paul S, Philipp U, et al.Sequence analysis of a 212 kb defensin gene cluster on ECA 27q17[J].Gene, 2006,376(2):192～198.
29 Luenser K, Fickel J, Ludwig A.Evolution of caprine and ovine beta-defensin genes[J]. Immunogenetics, 2005, 57(7):487～498.
30 Mallow E B, Harris A, Salzman N, et al.Human enteric defensins. Gene structure and development expression[J].J Biol Chem, 1996, 271(8):403.
31 Milenkovic D, Oustry-Vaiman A, Lear T L, et al.Cytogenetic localization of 136 genes in the horse: Comparative mapping with the human genome[J].Mamm Genome, 2002, 13(9):524～534.
32 Nava G M, Escorcia M, Cantaeda M P.Molecular diversity of the antimicrobial domain of beta-defensin 3 and homologous peptides.Comparative and Functional Genomics,2009.
33 Patil A, Hughes A L, Zhang G.Rapid evolution and diversification of mammalian α-defensins as revealed by comparative analysis of rodent and primate genes[J].Physiol Geno bmics, 2004,20(11):1～11.
34 Pawlik A, Sender G, Korwin-Kossakowska A.Bovine lactoferrin gene polymorphism and expression in relation to mastitis resistance a review[J].Anim Sci Pap Rep, 2009,27:263～271.
35 Rice W G, Ganz T, Kinkade J M, et al.Defensin-rich dense granules of human neutrophils[J].Blood, 1987,70(3):757～765.
36 Roosen S, Exner K, Paul S, et al.Bovine beta-defensins: Identification and characterisation of novel bovine beta-defensin genes and their expression in mammary gland tissue[J].Mamm Genome, 2004,15(10):834～842.
37 Ryan L K, Rhodes J, Bhat M, et al.Expression of beta-defensin genes in bovine alveolar macrophages[J].Infect Immun,1998,66(2):878～881.
38 Ryniewicz Z, Zwierzchowski L, Bagnicka E, et al.Association of the polymorphism at defensin gene loci with dairy production traits and milk somatic cell count in Black-and-White cows[J].Anim Sci Pap Rep, 2003,21:209～222.
39 Sang Y, Patil A A, Zhang G, et al.Bioinformatic and expression analysis of novel porcine beta-defensins[J].Mamm Genome, 2006,17(4):332～339.
40 Sang Y, Blecha F.Porcine host defense peptides: Expanding repertoire and functions[J].Dev Comp Immunol, 2009,33(3):334～343.
41 Schonwetter B S, Stolzenberg E D, Zasloff M A.Epithelial antibiotics induced at sites of inflammation[J].Science, 1995, 267(5204):1645～1648.
42 Selsted M E, Tang Y Q, Morris W L, et al.Purification, promary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils[J].J Biol Chem, 1993,268(9):6641～6648.
43 Shi J, Zhang G, Wu H, et al.Porcine epithelial beta-defensin 1 is expressed in the dorsal tongue at antimicrobial concentrations[J].Infect Immun, 1999, 67(6): 3121～3127.
44 Stolzenberg E D, Anderson G M, Ackermann M R, et al.Epithelial antibiotic induced in states of disease[J].Proc Natl Acad Sci USA, 1997,94(6):8686～8690.
45 Sun C Q, Arnold R, Fernandez-Golarz C, et al.Human beta-defensin-1, a potential chromosome 8p tumor suppressor:Control of transcription and induction of apoptosis in renal cell carcinoma[J].Cancer Res, 2006,66(17):8542～8549.
46 Swanson K, Gorodetsky S, Good L, et al.Expression of a beta-defensin mRNA, lingual antimicrobial peptide, in bovine mammary epithelial tissue is induced by mastitis[J].Infect Immun, 2004,72(12):7311～7314.
47 Tang Y Q, Yuan J, Osapay G,et al.A cyclic antimicrobial peptide produced in primate leucocytes by the ligation of two truncated alpha-defensin[J].Science,1999,286(5439):489～502.
48 Tarver A P,Clark D P,Diamond G,et al.Enteric beta-defensin: Molecular cloning and characterisation of a gene with inducible intenstinal epithelial cell expression associated with Cryptosporidium parvum infection[J].Infect Immun, 1998, 66(3):1045～1056.
49 Veldhuizen E J Q, Rijnders M, Claassen E A, et al.Porcine beta-defensin 2 displays broad antimicrobial activity agains pathogenic intestinal bacteria[J].Mol Immunol, 2008,45(2):386～394.
50 Wauchula B E,Tustanowski J P,Martirosian G.Antimicrobial peptides[J].Wiad Lek, 2006,59:542～547.
51 Wojdak-Maksymiec K,Kmiec M,Zukiewicz A.Associations between defensin polymorphism and somatic cell count in milk and milk utility traits in Jersey dairy cows[J].J Vet Med A Physiol Pathol Clin Med, 2006,53(10):495～500.
52 Zhang G,Hiraiwa H,Yasue H,et al.Cloning and characterisation of the gene for a new epithelial beta-defensin.Genomic structure, chromosomal localiz-ation, and evidence for its constitutive expression[J].J Biol Chem, 1999,274(34):24031～24037.

[1]	韦明邦, 边巴琼达, 肖青青, 段梦琪, 强巴央宗, 商鹏. 藏猪CDKN1B基因克隆、生物信息学及表达分析[J]. 中国畜牧兽医, 2023, 50(6): 2196-2206.
[2]	肖鹏, 尚江华, 杨春艳, 李孟琪, 段安琴, 马小娅, 冯超, 黄晨茜, 张博, 周金陈, 韦科龙, 郑威, 郑海英. α-亚麻酸对水牛卵巢颗粒细胞体外培养的影响[J]. 中国畜牧兽医, 2023, 50(6): 2380-2387.
[3]	焦琳琳, 成玉婷, 吴庆国, 吴双, 吴植, 朱善元, 钱莺娟. 鸭坦布苏病毒Capsid蛋白原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2395-2402.
[4]	李灿, 李楚楚, 曾维, 张宁, 纪春晓, 陈韬. 猪RegⅢγ蛋白的重组表达及功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2414-2426.
[5]	宋越, 苏胜杰, 张帆, 白帆, 戴伶俐, 王娜, 王大伟, 杨茜雯, 赵世华, 张月梅. 溶血性曼氏杆菌截短型白细胞毒素的原核表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2479-2486.
[6]	欧云文, 潘琴, 汪洋, 代军飞, 任绍科, 张洋, 张杰. 猪细小病毒6型ORF2基因的截短表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2487-2495.
[7]	王浩睿, 禹保军, 杨嘉翊, 魏榕, 付希, 王川川, 张迪, 李德生, 蔡正云, 顾亚玲, 张娟. 静原鸡DERA基因生物信息学分析及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1764-1773.
[8]	潘鹏丞, 雷宗全, 李显, 胡湘云, 秦谦涛, 覃兆鲜, 关志惠, 陈宝剑, 谢炳坤. 陆川猪MYL2基因生物信息学分析、真核表达载体构建及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1796-1806.
[9]	王泽平, 王飞飞, 张芮铭, 付言峰, 赵为民, 戴超辉, 程金花, 廖超, 李辉. 苏紫猪SLA-1基因多态性及其抗病潜能分析[J]. 中国畜牧兽医, 2023, 50(5): 1918-1927.
[10]	卓娜, 朱忠武, 李静, 胡世享, 王建昌, 林华, 陈朝林, 韩佃刚, 艾军, 陈培富. 猪源戊型肝炎病毒ORF2蛋白生物信息学分析及真核表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1959-1970.
[11]	张芳源, 蔺雅婷, 杨大为, 陈虎, 李桂梅, 单虎. 非洲猪瘟病毒P30蛋白原核表达及间接ELISA检测方法建立[J]. 中国畜牧兽医, 2023, 50(5): 2012-2022.
[12]	王雪平, 张孝俊, 李晓月, 王国栋, 张福良, 宋玉伟, 张明亮, 马磊. 高致病性禽腺病毒血清4型Hexon蛋白的原核表达及多克隆抗体的制备[J]. 中国畜牧兽医, 2023, 50(5): 2053-2060.
[13]	陶庆华, 黄彩云, 陈超, 刘威, 孙宁宁, 李昂. 白羽番鸭LIF基因克隆及其组织表达差异分析[J]. 中国畜牧兽医, 2023, 50(4): 1296-1306.
[14]	刘红润, 朱思燃, 奉玲丽, 张坤, 颜港, 王钰滨, 张帅, 江山, 许迪, 兰干球, 梁晶. 大白猪CDO1基因克隆、生物信息学分析及组织表达定位[J]. 中国畜牧兽医, 2023, 50(4): 1352-1363.
[15]	张蕊, 穆春宇, 汤青萍, 卜柱, 杨明军, 夏爱萍, 杨晓明. 鸽GnIH基因多态性及其与产蛋性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1412-1421.