槲皮素对蒙古羊卵泡发育、卵泡液微环境及卵巢类固醇生成相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2025.03.017

摘要/Abstract

摘要： 【目的】探索槲皮素对母羊卵泡发育、免疫及抗氧化功能、卵泡液生殖激素分泌和卵巢类固醇生成相关基因的影响。【方法】本试验选取体重相近、经产蒙古羊母羊30只，随机分为2组：对照组和槲皮素组，对照组饲喂基础饲粮，槲皮素组每只羊在基础饲粮中添加槲皮素7 g/d，每组3个重复，每个重复5只羊单独一栏饲养。试验采用同期发情处理，在第2个情期第13天采集血液用于抗氧化指标和生殖激素指标检测，采集母羊卵巢组织用于类固醇激素生成相关基因表达的检测。【结果】①相比于对照组，槲皮素组母羊的大卵泡和中卵泡数显著增加(P＜0.05)，血清中白细胞介素-6、肿瘤坏死因子、免疫球蛋白G和免疫球蛋白A的水平极显著或显著降低(P＜0.01；P＜0.05)，同时血清中总抗氧化能力、超氧化物歧化酶和谷胱甘肽过氧化物酶的活力极显著或显著升高(P＜0.01；P＜0.05)。②相比于对照组，槲皮素组母羊大卵泡内雌二醇、孕酮、促卵泡生成素和促性腺激素释放激素水平极显著或显著升高(P＜0.01；P＜0.05)，中等卵泡内雌二醇和促卵泡生成素水平极显著升高(P＜0.01)；各级卵泡内促黄体生成素浓度均显著或极显著降低(P＜0.05；P＜0.01)。③相比于对照组，饲粮添加槲皮素极显著上调了母羊卵巢类固醇激素合成急性调节蛋白(steroidogenic acute regulatory protein，STAR)和细胞色素P450家族19A成员1(cytochrome P450 family 19 subfamily A member 1，CYP19A1)的mRNA表达(P＜0.01)。【结论】饲粮添加7 g/d槲皮素可有效提高母羊抗炎症和抗氧化功能，并通过调控卵泡液生殖激素分泌和卵巢类固醇生成相关基因表达，进而影响母羊卵泡发育。

关键词: 槲皮素; 卵泡发育; 卵泡液微环境; 类固醇激素; 基因表达

Abstract: 【Objective】 The aim of this study was to explore the effects of quercetin on follicular development,immune and antioxidant function,follicular fluid reproductive hormone secretion and ovarian steroidogenesis related genes in ewes.【Method】 In this study,thirty Mongolian ewes with similar body weight were randomly divided into two groups:Control group and quercetin group.The control group of ewes were fed with basic diet,and each ewe in quercetin group was fed a basal diet supplemented with quercetin 7 g/d.Each group had three replicates,and five sheep in each replicate were raised in a separate pen.Blood samples were collected on the 13th day of the second estrus stage for the detection of antioxidant indexes and reproductive hormone indexes,and ovarian tissues were collected for the detection of steroidogenesis related gene expression.【Result】 ①Compared with control group,the number of large and middle follicles in quercetin group increased significantly(P＜0.05),the levels of interleukin-6,tumor necrosis factor,immunoglobulin G,and immunoglobulin A in serum of ewes in quercetin group were extremely significantly or significantly reduced (P＜0.01 or P＜0.05),while the total antioxidant capacity,superoxide dismutase,and glutathione peroxidase activities in serum were extremely significantly or significantly increased (P＜0.01 or P＜0.05).②Compared with control group,the levels of estrogen,progesterone,follicle stimulating hormone and gonadotropin-releasing hormone in the large follicles of ewes in quercetin group were extremely significantly or significantly higher than those in control group(P＜0.01 or P＜0.05).The levels of estrogen and follicle stimulating hormone in medium follicles were extremely significantly increased(P＜0.01).The levels of luteinizing hormone were significantly or extremely significantly reduced in the follicles at different grades (P＜0.05 or P<0.01).③Compared with control group,the mRNA expression of steroidogenic acute regulatory protein (STAR) and cytochrome P450 family 19 subfamily A member 1 (CYP19A1) in ewe ovaries were extremely significantly upregulated by adding quercetin to the feed (P＜0.01).【Conclusion】 Adding 7 g/d quercetin to the feed could effectively improve the anti-inflammatory and antioxidant functions of ewes,and regulate the secretion of reproductive hormones in follicular fluid and the expression of genes related to ovarian steroid production,thereby affecting the development of ewe follicles.

Key words: quercetin; follicular development; follicular fluid microenvironment; steroid hormones; gene expression

中图分类号:

S814.1

王泳, 那日苏, 梁建勇, 刘洋, 李九月, 高源, 田丰, 薛树媛. 槲皮素对蒙古羊卵泡发育、卵泡液微环境及卵巢类固醇生成相关基因表达的影响[J]. 中国畜牧兽医, 2025, 52(3): 1150-1158.

WANG Yong, NA Risu, LIANG Jianyong, LIU Yang, LI Jiuyue, GAO Yuan, TIAN Feng, XUE Shuyuan. Effects of Quercetin on Follicular Development,Follicular Fluid Microenvironment and Ovarian Steroidogenesis Related Gene Expression in Mongolian Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1150-1158.

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