不同浓度鞣花酸对猪精液常温保存效果的影响

doi:10.16431/j.cnki.1671-7236.2024.10.019

摘要/Abstract

摘要： 【目的】研究鞣花酸(ellagic acid，EA)对公猪精液常温保存过程中精子质量及抗氧化性能的影响，以期为鞣花酸在精液保存中的推广应用提供理论依据。【方法】试验采集4头2～3岁健康的成年杜洛克公猪精液，将合格精液分为5份，用添加不同浓度EA(0(对照)、5、10、15、20 μg/mL)的BTS(Beltsville thawing solution)常温保存稀释液分别稀释，在保存的第1、3、5、7天检测精子活率、平均路径速度、侧摆幅度、鞭打频率、质膜完整率、顶体完整率及抗氧化能力等指标。【结果】精液保存第1天，15 μg/mL EA组精子质膜完整率显著高于对照组(P＜0.05)，其他指标各试验组与对照组均无显著性差异(P＞0.05)。精液保存第3天，10、15 μg/mL EA组精子平均路径速度、过氧化氢酶(CAT)活性、总抗氧化能力(T-AOC)显著高于对照组(P＜0.05)。精液保存第5天，15、20 μg/mL EA组精子侧摆幅度显著低于对照组及5、10 μg/mL EA组(P＜0.05)，5、10、15 μg/mL EA组精子活率显著高于对照组(P＜0.05)，10、15、20 μg/mL EA组精液中CAT、谷胱甘肽过氧化物酶(GSH-Px)活性及T-AOC显著高于对照组(P＜0.05)。精液保存第7天，各试验组精子活率、平均路径速度、质膜完整率、顶体完整率、CAT活性、T-AOC均显著高于对照组(P＜0.05)。【结论】添加EA于BTS稀释液中，对常温保存的精液具有良好的保护作用，其中10 μg/mL EA效果最好。

关键词: 鞣花酸; 常温保存; 精液品质; 抗氧化能力; 猪

Abstract: 【Objective】 The aim of this study was to investigate the effects of ellagic acid (EA) on the quality and antioxidant properties of boar semen during room temperature preservation，in order to provide a theoretical basis for the promotion and application of EA in semen preservation.【Method】 Semen was collected from four healthy adult Duroc boars aged 2 to 3 years. The qualified semen was divided into five portions and diluted with BTS (Beltsville thawing solution) diluted at room temperature，which contained different concentrations of EA (0 (control),5，10，15 and 20 μg/mL). Sperm motility，average path velocity，swing amplitude，whipping frequency，plasma membrane integrity rate，acrosome integrity rate，and antioxidant capacity were assessed on the 1st，3rd，5th，and 7th days of preservation. 【Result】 On the 1st day of preservation，plasma membrane integrity was significantly higher in 15 μg/mL EA group compared to control group (P＜0.05)，and there was no significant difference in other indicators among experimental groups and control group (P＞0.05). On the 3rd day of semen preservation，the average path velocity，catalase (CAT) activity，and total antioxidant capacity (T-AOC) of sperm in 10 and 15 μg/mL EA groups were significantly higher than those in control group (P＜0.05). On the 5th day of semen preservation，the swing amplitude of sperm in 15 and 20 μg/mL EA groups was significantly lower than that in control group and the 5 and 10 μg/mL EA groups (P＜0.05)，and the sperm motility in 5，10 and 15 μg/mL EA groups was significantly higher than that in control group (P＜0.05). The CAT，glutathione peroxidase (GSH-Px) activitives，and T-AOC in the semen of 10，15 and 20 μg/mL EA groups were significantly higher than those in control group (P＜0.05). On the 7th day of semen preservation，the sperm motility，average path velocity，plasma membrane integrity，acrosome integrity，CAT activity，and T-AOC in each experimental group were significantly higher than those in control group (P＜0.05). 【Conclusion】 The incorporation of EA into BTS dilution solution was found to offer a protective effect on semen during room-temperature storage，with the optimal outcome observed at an EA concentration of 10 μg/mL.

Key words: ellagic acid; room temperature preservation; semen quality; antioxidant ability; pig

中图分类号:

S814.3

屈先先, 舒亮, 孙小晶, 刘梅, 高凤仙, 杨泰. 不同浓度鞣花酸对猪精液常温保存效果的影响[J]. 中国畜牧兽医, 2024, 51(10): 4383-4390.

QU Xianxian, SHU Liang, SUN Xiaojing, LIU Mei, GAO Fengxian, YANG Tai. Effect of Different Concentrations of Ellagic Acid on Preservation of Pig Semen at Room Temperature[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(10): 4383-4390.

参考文献

[1] LEE S H，PARK C K. Antioxidative effects of magnetized extender containing bovine serum albumin on sperm oxidative stress during long-term liquid preservation of boar semen[J].Biochemical & Biophysical Research Communications，2015，464(2):467-472.
[2] 吴小磊,钟晨,史金铭.鞣花酸的生物学效应[J].中国林副特产,2019，1:73-78.WU X L,ZHONG C,SHI J M. Biological effect of ellagic acid[J]. Forest By-Product and Speciality in China,2019，1:73-78.(in Chinese)
[3] CORBETT S，DANIEL J，DRAYTON R，et al. Evaluation of the anti-inflammatory effects of ellagic acid[J]. Journal of Perianesthesia Nursing，2010，25(4):214-220.
[4] VATTEM D，SHETTY K. Biological functionality of ellagic acid: A review[J]. Journal of Food Biochemistry， 2005，29(3):234-266.
[5] AJINA M，ATIG F，ZIDI I，et al. Effects of seminal glutathione peroxidase and its related non-enzymatic antioxidants on sperm DNA integrety and semen quality[J]. Fertility and Sterility，2013,100(3):S225-S226.
[6] 刁玉林,朱冬青,刘斌.秀丽莓中鞣花酸的含量测定和抗氧化活性评测及对接研究[J].西北药学杂志,2018,33(1):1-5.DIAO Y L,ZHU D Q,LIU B. Content determination,antioxidant activity estimation and docking study of ellagic acid in Rubus amabilis[J]. Northwest Pharmaceutical Journal,2018,33(1):1-5.(in Chinese)
[7] SINGH B，SINGH J，KAUR A，et al. Antimicrobial potential of pomegranate peel: A review[J]. International Journal of Food Science and Technology，2018,54(4):959-965.
[8] WABERSKI D，SUAREZ S S ，HENNING H. Assessment of sperm motility in livestock: Perspectives based on sperm swimming conditions in vivo[J].Animal Reproduction Science，2022，246(11):106849.
[9] BROEKHUIJSE M L W J ，SOSTARIC E ，FEITSMA H,et al. Application of computer-assisted semen analysis to explain variations in pig fertility[J].Journal of Animal Science，2012，90(3):779-789.
[10] VLASIC H，VLASTELIC I，RANDIC L,et al. Sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro[A].13th World Congress on In Vitro Fertilization，Assisted Reproduction and Genetics[C].2005.
[11] NAJAFI A，TAHERI R A ，MEHDIPOUR M ,et al.Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes[J].Oxford University Press，2019，98(1):440-446.
[12] BUCAK M N，BODU M，BASPINAR N,et al. Influence of ellagic acid and ebselen on sperm and oxidative stress parameters during liquid preservation of ram semen[J].Cell Journal，2019，21(1):7.
[13] OMUR A D，OYAN K，OZTURK C,et al. The effects of curcumin，ellagic acid and methionine on post-thawed Merino rams sperm parameters[J].The FASEB Journal，2014,28：759-761.
[14] KIA H D，BLOUKI Z，DODRAN H V,et al. Effect of adding coenzyme Q10 and ellagic acid during cryopreservation on post-thaw quality of ram semen[J]. Iranian Journal of Applied Animal Science，2017,7(3):445-451.
[15] GAO Y，PENG K，WANG Y，et al. Ellagic acid ameliorates cisplatin-induced acute kidney injury by regulating inflammation and SIRT6/TNF-α signaling[J]. Food Science and Human Wellness，2023,12(6):2232-2241.
[16] 尹培培,闫林林,曹若愚,等.鞣花酸代谢产物——尿石素的研究进展[J].食品科学,2015,36(7):256-260.YIN P P，YAN L L，CAO R Y，et al. A review of urolithins，gut microflora metabolites of dietary ellagic acid[J]. Food Science，2015,36(7):256-260.(in Chinese)
[17] SZABLICKA D，WYSOKINSKA A，PAWLAK A,et al. Morphometry of boar spermatozoa in semen stored at 17℃—The influence of the staining technique[J]. Animals, 2022,12(15):1888.
[18] FOROUZANFAR M，SHARAFI M，HOSSEINI S M，et al. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen[J].Theriogenology，2010，73(4):480-487.
[19] WANG X Z，XIA L，ZHANG X Y,et al. The multifaceted mechanisms of paeoniflorin in the treatment of tumors: State-of-the-Art[J].Biomedicine & Pharmacotherapy，2022，149:112800.
[20] OMUR A D，COYAN K.Protective effects of the antioxidants curcumin，ellagic acid and methionine on motility，mitochondrial transmembrane potential，plasma membrane and acrosome integrity in freeze-thawed Merino ram sperm[J].Veterinární Medicína,2016,61(1):10-16.
[21] 任发.鞣花酸对猪精液常温保存效果的研究[D].杨凌：西北农林科技大学,2018.REN F. Effect of ellagic acid on boar semen quality during normal temperature preservation[D].Yangling：Northwest A&F University,2018.(in Chinese)
[22] AKHTAR M F，MA Q，LI Y，et al. Effect of sperm cryopreservation in farm animals using nano technology[J]. Animals，2022,12(17):2277.
[23] IOVINE C，MOTTOLA F ，SANTONASTASO M,et al. In vitro ameliorative effects of ellagic acid on vitality，motility and DNA quality in human spermatozoa[J].Molecular Reproduction and Development，2021,88(2):167-174.
[24] MOTTOLA F，IOVINE C，CARRANNANTE M，et al. In vitro combination of ascorbic and ellagic acids in sperm oxidative damage inhibition[J].International Journal of Molecular Sciences，2022,23(23):14751.
[25] ZHANG X，WANG S，WU Y，et al. Ellagic acid alleviates diquat—Induced jejunum oxidative stress in C57BL/6 mice through activating Nrf2 mediated signaling pathway[J]. Nutrients，2022,14(5):1103.
[26] XU Q，SHEN M,HAN Y，et al. Effects of ellagic acid supplementation on jejunal morphology，digestive enzyme activities，antioxidant capacity，and microbiota in mice[J]. Frontiers in Microbiology，2021,12:793576.
[27] OBID N M A，ARRAK J K. Protective effect of ellagic acid on epididymal sperms quality and DNA deformity in mature rats exposed to cadmium chloride[J]. University of Thi-Qar Journal of Agricultural Research，2023,12(1):165-180.