三穗鸭SLC4A9基因表达量及其多态性对蛋壳品质的遗传效应分析

doi:10.16431/j.cnki.1671-7236.2021.06.021

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (6): 2082-2090.doi: 10.16431/j.cnki.1671-7236.2021.06.021

三穗鸭SLC4A9基因表达量及其多态性对蛋壳品质的遗传效应分析

杨酸^1,2, 张依裕^1,2, 谭元成^1,2, 王盈童^1,2, 游敏芳^1,2, 廖朝美^1,2

1. 贵州大学动物科学学院, 高原山地动物遗传育种与繁殖教育部重点实验室, 贵阳 550025;
2. 贵州大学, 贵州省动物遗传育种与繁殖重点实验室, 贵阳 550025

收稿日期:2020-12-30 出版日期:2021-06-20 发布日期:2021-06-18
通讯作者: 张依裕 E-mail:zyy8yyc@163.com
作者简介:杨酸(1998-),女,贵州龙里人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:1535390590@qq.com
基金资助:
国家自然科学基金"IP3Rs通道调控蛋鸭蛋壳品质的效应机制研究"（31760663）；贵州省科技人才补助项目（黔科合平台人才[2017]5788）

Genetic Effects Analysis of Polymorphism and Expression of SLC4A9 Gene on Eggshell Quality in Sansui Ducks

YANG Suan^1,2, ZHANG Yiyu^1,2, TAN Yuancheng^1,2, WANG Yingtong^1,2, YOU Minfang^1,2, LIAO Chaomei^1,2

1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction of Guizhou Province, Guizhou University, Guiyang 550025, China

Received:2020-12-30 Online:2021-06-20 Published:2021-06-18

摘要/Abstract

摘要： 为探究溶质载体家族4成员9（solute carrier family 4 member 9，SLC4A9）基因的表达量及其多态性对鸭蛋壳品质的遗传效应，试验采用实时荧光定量PCR法检测SLC4A9基因在三穗鸭不同组织中的表达水平，采用PCR产物直接测序法筛查三穗鸭SLC4A9基因的SNP位点，并分析SNP位点对三穗鸭蛋壳品质的遗传效应。结果显示，SLC4A9基因mRNA在三穗鸭10个组织中均有不同程度表达，其中在心脏和胰腺中为高度特异性表达，肾脏中为中度表达，其他为低度表达。试验共检测到10个SNPs位点，其中3个位于外显子上（g.6803 C>T、g.7065 C>T和g.7089 C>G），且均为错义突变，7个位于内含子上（g.7162 C>G、g.11044 G>A、g.11090 T>C、g.11234 C>T、g.11261 C>T、g.11349 C>T和g.11403 G>A），其中g.6803 C>T位点突变导致丙氨酸（GCG）变为缬氨酸（GTG）；g.7065 C>T位点突变导致丙氨酸（GCC）变为缬氨酸（GTC）；g.7089 C>G位点突变导致丙氨酸（GCA）变为甘氨酸（GGA）。χ²检验结果显示，g.6803 C>T、g.11090 T>C和g.11349 C>T突变位点的基因型分布均偏离了Hardy-Weinberg平衡状态（P<0.05），其余位点的基因型分布均符合Hardy-Weinberg平衡状态（P>0.05）。关联分析结果表明，g.11349 C>T位点的CC和CT基因型蛋壳强度显著高于TT基因型（P<0.05）。综上，SLC4A9基因的10个SNPs位点均对三穗鸭的蛋壳品质产生影响，其中g.11349 C>T位点达到了显著水平，可为蛋鸭蛋壳品质的改善及遗传标记提供参考依据。

关键词: 三穗鸭; SLC4A9基因; 组织表达; 多态性; 蛋壳品质

Abstract: In order to explore the genetic effects of solute carrier family 4 member 9 (SLC4A9) expression and polymorphism on the eggshell quality in duck,the expression levels of SLC4A9 gene in different tissues of Sansui ducks were detected by Real-time quantitative PCR,the SNP of SLC4A9 gene in Sansui ducks were directly sequenced by PCR products,and the genetic effect of SNP on the eggshell quality in Sansui ducks was analyzed.The results showed that the SLC4A9 gene mRNA was expressed to varying degrees in 10 tissues of Sansui ducks,with highly specific expression in heart and pancreas,moderate expression in kidney,and low expression in others.A total of 10 SNPs were detected among which three were located on exons and all were missense mutations(g.6803 C>T,g.7065 C>T and g.7089 C>G),and seven were located in introns (g.7162 C>G,g.11044 G>A,g.11090 T>C,g.11234 C>T,g.11261 C>T,g.11349 C>T and g.11403 G>A).Among them,g.6803 C>T mutation led to alanine (GCG) turning into valine (GTG),g.7065 C>T mutation caused alanine (GCC) to valine (GTC),g.7089 C>G mutation caused alanine (GCA) to glycine (GGA).χ² test found that g.6803 C>T,g.11090 T>C and g.11349 C>T deviated from Hardy-Weinberg equilibrium (P<0.05),and the genotype distribution of the other SNPs were in accordance with Hardy-Weinberg equilibrium (P>0.05).Correlation analysis results showed that the eggshell strength of CC and CT genotypes at g.11349 C>T was significantly higher than that of TT genotype (P<0.05).In conclusion,10 SNPs of SLC4A9 gene all affected the eggshell quality of Sansui ducks,and g.11349 C>T reached a significant level,which could provide reference for the improvement of eggshell quality and the selection of genetic markers in ducks.

Key words: Sansui ducks; SLC4A9 gene; organization expression; polymorphism; eggshell quality

中图分类号:

S834

杨酸, 张依裕, 谭元成, 王盈童, 游敏芳, 廖朝美. 三穗鸭SLC4A9基因表达量及其多态性对蛋壳品质的遗传效应分析[J]. 中国畜牧兽医, 2021, 48(6): 2082-2090.

YANG Suan, ZHANG Yiyu, TAN Yuancheng, WANG Yingtong, YOU Minfang, LIAO Chaomei. Genetic Effects Analysis of Polymorphism and Expression of SLC4A9 Gene on Eggshell Quality in Sansui Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2082-2090.

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三穗鸭SLC4A9基因表达量及其多态性对蛋壳品质的遗传效应分析

Genetic Effects Analysis of Polymorphism and Expression of SLC4A9 Gene on Eggshell Quality in Sansui Ducks

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