TMEM219基因可变剪切体相对表达量及其对鹿茸重量的影响

doi:10.16431/j.cnki.1671-7236.2020.07.020

摘要/Abstract

摘要： 试验旨在研究TMEM219基因3种剪切体在不同重量鹿茸尖端的表达规律及TMEM219基因表达对鹿茸重量的影响，以期探究TMEM219基因对鹿茸生长发育的调控机理。利用实时荧光定量PCR技术对TMEM219基因及其3种剪切体在同一重量组鹿茸的不同组织及不同重量组鹿茸的同一组织mRNA的相对表达水平进行检测，同时测定并比较不同产茸量梅花鹿的血清中胰岛素生长因子1（IGF-1）和胰岛素样生长因子结合蛋白3（IGFBP-3）的浓度。结果表明，TMEM219基因的3种剪接体在梅花鹿鹿茸的间充质及前成软骨组织（RP）、过渡组织（TZ）及软骨组织（C）中均有表达，TMEM219-918基因相对表达量极显著高于TMEM219-1005与TMEM219-1960基因（P<0.01），TMEM219-1005与TMEM219-1960基因相对表达量无显著差异（P>0.05）；高重量组TMEM219基因表达量显著高于低重量组（P<0.05）；同时，高重量组个体血清中IGF-1浓度显著高于低重量组个体（P<0.05），而IGFBP-3浓度显著低于低重量组个体（P<0.05）。结果提示，TMEM219基因高表达可能会促进鹿茸的生长，增加鹿茸重量；推测其可能的机理是TMEM219竞争性结合IGFBP-3，减少与其结合的IGF-1，加强IGF-1与IGF-1R的亲和力，进而提高IGF-1对鹿茸生长的促进作用。TMEM219基因可能成为影响鹿茸生长发育的候选基因，为提高鹿茸生长提供理论基础。

关键词: 鹿茸; TMEM219基因; 可变剪切体; 荧光定量

Abstract: The purpose of the experiment was to study the expression patterns of three kinds of TMEM219 gene spliced antler at different weights and the effect of TMEM219 gene expression on the weight of velvet antler,in order to explore the regulatory mechanism of TMEM219 gene on the growth and development of velvet antler.The Real-time quantitative PCR technology was used to detect the relative expression levels of TMEM219 gene and its three spliced variants in different tissues of the same weight group and the same tissue of different weight groups of velvet antler.Simultaneously,the serum levels of insulin growth factor 1 (IGF-1) and insulin-like growth factor binding protein 3 (IGFBP-3) were measured and compared of Sika deer serum with different antler yields.The results showed that three kinds of spliceosome of TMEM219 gene were expressed in reserve mesenchyme and precartilage (RP),transition zone (TZ) and cartilage (C) of Sika deer antler.The relative expression of TMEM219-918 was extremely significantly higher than that of TMEM219-1005 and TMEM219-1960 (P<0.01),and there was no significant difference in the relative expression of TMEM219-1005 and TMEM219-1960 (P>0.05).The expression of TMEM219 gene in the high weight group was significantly higher than that in the low weight group (P<0.05).At the same time,the concentration of IGF-1 in the serum of high weight group was significantly higher than that of low weight group (P<0.05),while the IGFBP-3 concentration was significantly lower than that of low weight group (P<0.05).The results suggested that the high expression of TMEM219 gene might promote the growth of velvet antler and increase the weight of velvet antler.It was speculated that the possible mechanism was that TMEM219 competitively binded to IGFBP-3,reduced its IGF-1 binding ability,and strengthened the IGF-1 and IGF-1R affinity,which in turn enhanced the growth promotion effect of IGF-1 on velvet antler.TMEM219 gene would become a candidate gene that affected the growth and development of velvet antler,providing a theoretical basis for improving the growth of velvet antler.

Key words: velvet antler; TMEM219 gene; variable shear; fluorescence quantitative

中图分类号:

S825

赵佩, 王磊, 王洪亮, 邢秀梅, 胡鹏飞. TMEM219基因可变剪切体相对表达量及其对鹿茸重量的影响[J]. 中国畜牧兽医, 2020, 47(7): 2150-2158.

ZHAO Pei, WANG Lei, WANG Hongliang, XING Xiumei, HU Pengfei. Relative Expression Level of TMEM219 Gene Variable Shear and Its Effect on Antler Weight[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2150-2158.

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