秦川牛ACTC1基因的表达特性分析

doi:10.16431/j.cnki.1671-7236.2020.08.003

摘要/Abstract

摘要： 试验旨在揭示ACTC1基因在秦川牛中的时空表达规律，为进一步研究ACTC1基因在肉牛肌肉发育和脂肪沉积等方面的功能奠定基础。利用实时荧光定量PCR技术检测了ACTC1基因在24月龄成年秦川牛13种组织和4日龄新生秦川牛12种组织中的表达规律，同时研究分析了该基因在秦川牛成肌细胞和前体脂肪细胞不同分化阶段（0、2、4、6、8 d）的表达特性。结果显示，ACTC1基因在秦川牛心脏中的表达量最高，骨骼肌中次之；除瘤胃和肾脏外，24月龄成年牛各组织中的表达量显著或极显著高于4日龄新生牛（P<0.05；P<0.01）；ACTC1基因在成肌细胞中的表达随着分化程度的增加呈现先升高后降低的趋势，在成肌细胞分化第2、4、6和8天ACTC1基因的表达量与第0天相比差异极显著（P<0.01），分别是第0天的2.6、4.7、5.6和4.2倍，这与成肌细胞的分化速率一致；在脂肪细胞中ACTC1基因的表达趋势为先降后升，第2、4天的表达量与第0天相比差异极显著（P<0.01），从分化第2天开始表达量随脂肪细胞分化程度增加而增加。ACTC1基因在不同年龄牛肌肉组织（心肌和骨骼肌）中的表达量最高，且其表达特性与肌细胞分化整体趋势一致；另外，ACTC1基因在脂肪细胞中的表达也有一定的规律。综上所述，推测ACTC1基因可能会影响牛肌肉组织的生长发育和脂肪沉积。

关键词: ACTC1基因; 秦川牛; 成肌细胞; 前体脂肪细胞; 实时荧光定量PCR

Abstract: The study was aimed to reveal the temporal and spatial expression of ACTC1 gene in Qinchuan cattle,which will lay the foundation for further research on the functions of ACTC1 gene in muscle development and fat deposition of beef cattle.Real-time quantitative PCR was used to detect the expression of ACTC1 gene in 13 tissues of 24-month-old adult Qinchuan cattle and 12 kinds of tissues of 4-day-old Qinchuan cattle.At the same time,the expression characteristics of this gene in myoblasts and preadipocytes of Qinchuan cattle at different differentiation stages (0,2,4,6 and 8 d) were studied and analyzed.The results showed that the expression of ACTC1 gene was the highest in heart of Qinchuan cattle,followed by skeletal muscle.Except for rumen and kidney,the expression of ACTC1 at 24-month-old adult cattle was significantly or extremely significantly higher than that at 4-day-old newborn cattle (P<0.05;P<0.01).The expression of ACTC1 gene in myoblasts increased at first and then decreased along with the myoblasts differentiation.On the 2nd,4th,6th and 8th day of myoblast differentiation,the expression of ACTC1 gene was significantly different from that on 0th day (P<0.01),which were 2.6,4.7,5.6,and 4.2 times of 0th day,respectively.This was consistent with the differentiation rate of myoblasts.The expression trend of ACTC1 gene in adipocytes decreased at first and then increased.The expression levels on 2nd day and 4th day were significantly different from those on 0th day (P<0.01).From the 2nd day of differentiation,the expression increased with the adipocyte differentiation.The expression of ACTC1 gene was the highest in muscle tissues (myocardium and skeletal muscle) of cattle,and its expression characteristics were consistent with the overall trend of muscle cell differentiation.In addition,there was also a certain rule of ACTC1 gene expression in adipocytes.In summary,it was speculated that ACTC1 gene might affect the growth and development of muscle tissue and fat deposition in cattle.

Key words: ACTC1 gene; Qinchuan cattle; myoblasts; preadipocytes; Real-time quantitative PCR

中图分类号:

S823.8⁺1

李安奇, 蒋蕾, 苏晓彤, 昝林森, 王洪宝. 秦川牛ACTC1基因的表达特性分析[J]. 中国畜牧兽医, 2020, 47(8): 2359-2367.

LI Anqi, JIANG Lei, SU Xiaotong, ZAN Linsen, WANG Hongbao. Expression Characteristics Analysis of ACTC1 Gene in Qinchuan Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2359-2367.

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