广西地区水牛乳微量元素含量及其影响因素研究

doi:10.16431/j.cnki.1671-7236.2020.01.035

摘要/Abstract

摘要： 水牛乳是中国南方地区重要奶源，为了解中国广西地区水牛乳中微量元素含量及不同因素如地域、品种和泌乳期对水牛乳中微量元素含量的影响，于广西南宁市兴宁区、邕宁区和钦州市灵山县共3个水牛场采集了摩拉、尼里2个品种，3个泌乳时期的水牛乳样品共60份，通过微波消解，利用电感耦合等离子体质谱检测了铬（Cr）、锰（Mn）、钴（Co）、铜（Cu）、砷（As）、硒（Se）、铯（Cs）、钡（Ba）、钼（Mo）、铅（Pb）、镉（Cd）、镍（Ni）共12种微量元素的含量，采用皮尔逊相关分析解析了12种元素含量之间的相关性，采用单因素方差分析比较了不同地域、品种和泌乳期水牛乳之间微量元素含量差异，采用多因素方差分析解析交互作用对元素含量的影响。结果表明，该法测定水牛乳12种微量元素含量的加标回收率在84.94%~120.91%之间。不同地域间水牛乳中Mn、Se、Cs、Ni含量差异显著（P<0.05），钦州市灵山县水牛乳中Mn、Cs含量较高，南宁市兴宁区水牛乳中Se、Ni含量较高；不同品种间水牛乳中Se、Ni含量差异显著（P<0.05），尼里水牛乳中Se、Ni含量高于摩拉水牛；不同泌乳期水牛乳中Mn、Co、Cu、Se、Mo含量差异显著（P<0.05），初乳中Co、Cu、Se含量较高，常乳中Mn含量较高，晚乳中Mo含量较高；地域与品种的交互作用对水牛乳中微量元素含量无显著影响。

关键词: 水牛乳; 微量元素; 地域; 品种; 泌乳期

Abstract: Buffalo milk was an important milk source in the South of China.In order to study the contents of trace elements in buffalo milk in Guangxi and the influence of different factors such as region,breed,lactation,60 samples of buffalo milk with 2 breeds in 3 lactation periods were collected from 3 buffalo farms in Xingning and Yongning district in Nanning and Lingshan district in Qinzhou,Guangxi province.The samples were digested by microwave digestion method.12 trace elements (Cr,Mn,Co,Cu,As,Se,Cs,Ba,Mo,Pb,Cd and Ni) were analyzed by inductively coupled plasma mass spectrometry method (ICP-MS).Correlation among 12 trace element contents was analyzed using Pearson correlation analysis.Differences in trace element contents between buffalo milk in different regions,breeds and lactation stages were compared by one-way analysis of variance.Multivariate analysis of variance was used to analyze the effect of interaction on trace elemental contents.The results showed that the spiked recovery of 12 trace elements in buffalo milk was 84.94% to 120.91%.The contents of Mn,Se,Cs and Ni in buffalo milk were significantly different among different regions (P<0.05).The contents of Mn and Cs in Lingshan were higher than those in other regions.The contents of Se and Ni in Xingning were higher than those in other region.The contents of Se and Ni in buffalo milk were significantly different between different breeds (P<0.05).The contents of Se and Ni in Nile buffalo were higher than that in Mora buffalo.The contents of Mn,Co,Cu,Se and Mo were significantly different among different lactation periods (P<0.05).The contents of Co,Cu and Se in colostrum were the highest among three lactation periods.The content of Mn in mature milk was higher than that in other lactation periods.The content of Mo in late milk was the highest among three lactation periods.The interaction between region and breed had no significant effect on the contents of trace elements in buffalo milk.

Key words: buffalo milk; trace element; region; variety; lactation

中图分类号:

S823.8⁺3

喻笑男, 刘宏艳, 郭晓青, 赵青余, 李发弟, 张军民. 广西地区水牛乳微量元素含量及其影响因素研究[J]. 中国畜牧兽医, 2020, 47(1): 296-305.

YU Xiaonan, LIU Hongyan, GUO xiaoqing, ZHAO Qingyu, LI Fadi, ZHANG Junmin. Study on the Contents of Trace Elements and the Influencing Factors in Buffalo Milk in Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 296-305.

参考文献

[1] 高玎玲.内蒙古四种家畜乳常量与微量元素测定及特征分析[D].呼和浩特:内蒙古农业大学,2017. GAN D L.Determination and character analysis of constant and trace elements in four kinds of domestic animals in Inner Mongolia[D].Hohhot:Inner Mongolia Agricultural University,2017.(in Chinese)
[2] 张永云,杨忠,李卫真,等.水牛乳和荷斯坦牛乳中五种常量元素含量的测定[J].动物医学进展,2011,32(6):72-76. ZHANG Y Y,YANG Z,LI W Z,et al.Determination of five major elements in buffalo milk and Holstein milk[J].Progress in Animal Medicine,2011,32(6):72-76.(in Chinese)
[3] 陈丹瑾,牟光庆,姜铁民,等.乳及乳制品中矿物质元素检测方法的研究进展[J].食品与机械,2015,3:246-250. CHEN D J,MOU G Q,JIANG T M,et al.Research progress on detection methods of mineral elements in milk and dairy products[J].Food and Machinery,2015,3:246-250.(in Chinese)
[4] 张玉玉,唐善虎,徐进勇,等.冷冻干燥/微波消解/ICP-MS法测定牦牛乳中的微量元素[J].食品科学,2009,30(18):356-358. ZHANG Y Y,TANG S H,XU J Y,et al.Determination of trace elements in yak milk by freeze drying/microwave digestion/ICP-MS[J].Food Science,2009,30(18):356-358.(in Chinese)
[5] 周雪巍.不同奶畜生乳、水和饲料中多元素测定方法研究及含量分析[D].兰州:甘肃农业大学,2015. ZHOU X W.Study on determination method and content analysis of multi-element in raw milk,water and feed of different dairy dnimals[D].Lanzhou:Gansu Agricultural University,2015.(in Chinese)
[6] MASOUMEH T J,ALIAGHAR V,FATEME S,et al.Analysis of aluminum,minerals and trace elements in the milk samples from lactating mothers in Hamadan,Iran[J].Journal of Trace Elements in Medicine and Biology,2018,50:8-15.
[7] EMEL ÖRÜN,S.SONGÜL YALÇIN,AYKUT O,et al.Zinc and copper concentrations in breast milk at the second month of lactation[J].Indian Pediatrics,2012,49(2):133-135.
[8] MELITA K,ABDU A,MILAN J,et al.Simultaneous determination of cadmium,mercury,lead,arsenic,copper,and zinc in human breast milk by ICP-MS/microwave digestion[J].Analytical Letters,2008,41(3):11-13.
[9] MOHD-TAUFEK N,CARTWRIGHT D,DARBIES M,et al.The Simultaneous analysis of eight essential trace elements in human milk by ICP-MS[J].Food Analytical Methods,2016,9(7):2068-2075.
[10] 徐敏,陆东林,马卫平,等.新疆双峰驼驼乳中矿物元素和维生素质量浓度检测[J].草食家畜,2014(4):68-71. XU M,LU D L,MA W P,et al.Detection of mineral element and vitamin mass concentration in Xinjiang bactrian camel milk[J].Herbivorous Livestock,2014(4):68-71.(in Chinese)
[11] MINA M,IOLANDA A,AMANDA M,et al.Gross,mineral and fatty acid composition of alpaca (Vicugna pacos) milk at 30 and 60 days of lactation[J].Small Ruminant Research,2015,5:50-54.
[12] NAKAMORI M,NINH N X,ISOMURA H,et al.Nutritional status of lactating mothers and their breast milk concentration of iron,zinc and copper in rural vietnam[J].Journal of Nutritional Science and Vitaminology,2009,55(4):338-345.
[13] JULIETA G,MARIA V B,MICHA H,et al.Multielemental isotopic fingerprint enables linking soil,water,forage and milk composition,assessing the geographical origin of Argentinean milk[J].Food Chemistry,2019,10:549-558.
[14] QIAN J,CHEN T,LU W,et al.Breast milk macro- and micronutrient composition in lactating mothers from suburban and urban Shanghai[J].Journal of Paediatrics and Child Health,2010,46(3):115-120.
[15] ZHOU X,QU X,ZHENG N,et al.Large scale study of the within and between spatial variability of lead,arsenic,and cadmium contamination of cow milk in China[J].Science of the Total Environment,2019,650:3054-3061.
[16] INES M T,TRANCOSO M A,ANTONIO P L M,et al.Original research:Chemical composition and mineral content of goat milk from four indigenous Portuguese breeds in relation to one foreign breed[J].International Journal of Dairy Technology,2010,63(4):516-522.
[17] KLEIN L D,BREAKEY A A,SCELZAB,et al.Concentrations of trace elements in human milk:Comparisons among women in Argentina,Namibia,Poland,and the United States[J].PLoS One,2017,12(8):e0183367.
[18] 赵家梅.奶水牛泌乳期的饲养管理[J].中国畜牧兽医文摘,2016,32(1):83. ZHAO J M.Feeding management of milk buffalo during lactation[J].China Animal Husbandry and Veterinary Abstracts,2016,32(1):83.(in Chinese)
[19] MASTROENI S S B S,OKADA I A,PATRICIA H C,RONDO,et al.Concentrations of Fe,K,Na,Ca,P,Zn and Mg in maternal colostrum and mature milk[J].Journal of Tropical Pediatrics,2006,52(4):272-276.
[20] FANTUZ F,FERRARO S,TODINI L,et al.Donkey milk concentration of calcium,phosphorus,potassium,sodium and magnesium[J].International Dairy Journal,2012,24(2):143-145.
[21] 何海成,付丽,刘广宇,等.火焰原子吸收法测定牛毛和牛奶中微量元素的含量及二者相关性[J].中国乳品工业,2007,35(3):29-31. HE H C,FU L,LIU G Y,et al.Determination of trace elements in bovine hair and milk by flame atomic absorption spectrometry and correlation[J].China Dairy Industry,2007,35(3):29-31.(in Chinese)
[22] 郑家三,刘健男,吴凌,等.泌乳奶牛不同泌乳阶段某些主要维生素与微量元素缺乏的调查研究[J].黑龙江八一农垦大学学报,2015,3(5):52-56. ZHENG J S,LIU J N,WU L,et al.Investigation on the deficiency of vitamins and trace elements in different lactation stages of lactating dairy cows[J].Journal of Heilongjiang Bayi Agricultural University,2015,3(5):52-56.(in Chinese)
[23] 李光辉.微量元素间及其与营养素之间的协同与拮抗作用[J].乳业科学与技术,2005,27(2):80-82. LI G H.Synergism and antagonism between trace elements and nutrients[J].Dairy Science and Technology,2005,27(2):80-82.(in Chinese)
[24] MORENO-ROJAS R,SANCHEZ-SEGARRA P J,CANARA-MARTOS F,et al.Multivariate analysis techniques as tools for categorization of Southern Spanish cheeses:Nutritional composition and mineral content[J].European Food Research and Technology,2010,231(6):841-851.
[25] ZHOU X,QU X,ZHAO S,et al.Analysis of 22 elements in milk,feed,and water of dairy cow,goat,and buffalo from different regions of China[J].Biological Trace Element Research,2017,176(10):120-129.
[26] MONIKA K,ZYGMUNT L,KOPERSKA N,et al.Content of macro- and microelements in goat milk in relation to the lactation stage and region of production[J].Journal of Elementology,2013,18(8):107-114.
[27] GAIGNON P,GELE M,HURTAUD C,et al.Characterization of the nongenetic causes of variation in the calcium content of bovine milk on French farms[J].Journal of Dairy Science,2018,101(16):4554-4569.
[28] 林波,黎颖,李玲,等.不同品种水牛乳中矿物元素的测定与分析[J].中国乳品工业,2014,42(3):15-17. LIN B,LI Y,LI L,et al.Determination and analysis of mineral elements in different varieties of buffalo milk[J].Chinese Dairy Industry,2014,42(3):15-17.(in Chinese)
[29] MATOS C,MOUTINHO C,ALMEIDA C,et al.Trace element compositional changes in human milk during the first four months of lactation[J].International Journal of Food Sciences and Nutrition,2014,65(5):547-551.
[30] HANNAN M A,DOGADKIN N N,ASHUR I A,et al.Copper,selenium,and zinc concentrations in human milk during the first three weeks of lactation[J].Biological Trace Element Research,2005,107(10):11-20.
[31] BILANDŽIĆ,SEDAK N,MARIJA Đokić,et al.Content of macro- and microelements in the milk of Croatian coldblood mares during lactation[J].Slovenian Veterinary Research,2014,51(7):171-177.
[32] AKINSOYINU A O,TEWE O O,MBA A U.Concentration of trace elements in milk of west African dwarf goats affected by state of lactation[J].Journal of Dairy Science,1979,62(4):921-924.
[33] 刘益丽,江明锋,江伟华,等.麦洼牦牛全泌乳期乳中矿物质元素变化规律研究[J].中国畜牧兽医,2014,41(6):112-116. LIU Y L,JIANG M F,JIANG W H,et al.Study on the change law of mineral elements in the whole lactating milk of Wheat yak[J].China Animal & Husbandry Veterinary Medicine,2014,41(6):112-116.(in Chinese)
[34] LIU H Y,WEI Y M,ZHANG Y Q,et al.The effectiveness of multi-element fingerprints for identifying the geographical origin of wheat[J].International Journal of Food Science and Technology,2017,52:1018-1025.
[35] MORGOUNOV A,HUGO F G,ABUGALIEVA A,et al.Iron and zinc grain density in common wheat grown in central Asia[J].Euphytica,2007,155(1-2):193-203.