地菍总黄酮的急性毒性及降糖活性研究

doi:10.16431/j.cnki.1671-7236.2020.01.032

摘要/Abstract

摘要： 本研究旨在探讨地菍总黄酮的急性毒性及对糖尿病小鼠的降血糖活性。试验以单次最大给药剂量（5.6 mg/g体重）和最大给药体积（40 μL/g体重）的地菍总黄酮混悬液对正常小鼠进行灌胃。24 h内给药2次后连续观察14 d，记录小鼠的存活情况及一般状态，给药14 d后采集小鼠血清并检测谷草转氨酶（AST）、谷丙转氨酶（ALT）活性及肌酐（Cre）、尿素氮（BUN）含量，计算小鼠心脏、肝脏、脾脏、肺脏、肾脏的质量系数。采用一次性腹腔注射0.160 mg/g体重链脲佐菌素（STZ）建立糖尿病小鼠模型，验证地菍总黄酮对糖尿病小鼠的保护作用。试验设空白组、模型组、二甲双胍组（0.6 mg/g体重）、地菍总黄酮高剂量、中剂量及低剂量组（1.2、0.8、0.53 mg/g体重），连续给药21 d后，测定各组小鼠空腹血糖（FBG）、体重、采食量、饮水量及24 h尿蛋白含量。地菍总黄酮急性毒性试验结果显示，地菍总黄酮灌胃给药的日最大给药量为11.2 mg/g体重；给药后14 d，与正常组相比，给药组小鼠体重、各脏器质量系数、血清中AST、ALT活性及Cre、BUN含量无显著差异（P>0.05）。在验证地菍总黄酮降血糖活性试验中，与模型组相比，地菍总黄酮给药组小鼠FBG、饮水量、采食量显著或极显著降低（P<0.05；P<0.01）。上述结果证实地菍总黄酮安全性高，未引起试验小鼠明显的急性毒性反应，并可有效降低糖尿病小鼠血糖含量，缓解小鼠糖尿病症状。

关键词: 地菍; 总黄酮; 急性毒性; 糖尿病

Abstract: This experiment was conducted to explore the acute toxicity and hypoglycemic activity in diabetic mice of total flavonoids from Melastoma dodecandrum Lour. (MDF).The mice were intragastrically administered with MDF solution (5.6 mg/(g·BW)) with the maximum gastric volume (40 μL/(g·BW)),solution was administered twice for 24 h.The state of mice in 14 d was recorded.At the end of the test,the levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT),creatinine (Cre) and urea nitrogen (BUN) were measured.The organ index was calculated by weighting the heart,liver,spleen,lung and kidney.The diabetic mouse model was established by intraperitoneal injection of 0.160 mg/(g·BW) streptozotocin (STZ).The mouse were divided into blank group,model group,metformin group (0.6 mg/(g·BW)),MDF high dose,medium dose and low dose groups (1.2,0.8,0.53 mg/(g·BW)).After 21 days of continuous administration,fasting blood glucose (FBG),body mass,feed intake,water intake and 24 h urine protein content of each group of mice were measured.The results showed that the maximum daily dose of MDF was 11.2 mg/(g·BW)).Compared with control group,there was no significant difference in body mass,organ index,and serum AST,ALT,Cre and BUN of the mouse after administration (P>0.05).In the hypoglycemic activity test,compared with the model group,the FBG,water and feed intake of MDF-treated group were significantly or extremely significantly reduced (P<0.05;P<0.01).The above results confirmed that the MDF was safe and non-toxic,it could effectively alleviate the symptoms and decrease blood glucose in diabetic mice.

Key words: Melastoma dodecandrum Lour.; total flavonoids; acute toxicity; diabetes

中图分类号:

S853.74

翁竞玉, 梁丽清, 周敬凯, 刘玉婷, 李丽. 地菍总黄酮的急性毒性及降糖活性研究[J]. 中国畜牧兽医, 2020, 47(1): 275-281.

WENG Jingyu, LIANG Liqing, ZHOU Jingkai, LIU Yuting, LI Li. Acute Toxicity and Hypoglycemic Activity of Total Flavonoids from Melastoma dodecandrum Lour.[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 275-281.

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