天津地区猪链球菌3型菌株的致病性及耐药特性研究

doi:10.16431/j.cnki.1671-7236.2019.12.034

摘要/Abstract

摘要： 试验旨在对分离自天津地区发病猪场的7株猪链球菌3型菌株（Streptococcus suis type 3，SS 3）进行致病性和耐药特性研究。应用剂量为1.0×10⁷、1.0×10⁸和1.0×10⁹ CFU/只的细菌对小鼠进行致病力研究，用PCR方法检测7株SS 3型菌株的毒力基因mrp、ef、sly、gdh、gapdh、fbps和orf2，并对这7株SS 3型菌株进行药物敏感试验。致病力试验结果显示，接种剂量为1.0×10⁹和1.0×10⁸ CFU/只时分别有6和3株SS 3型菌株可使小鼠100.0%（5/5）发病死亡，接种剂量1.0×10⁷ CFU时仍有1株SS 3型菌株可使80.0%（4/5）小鼠发病死亡，这7株SS 3型菌株对小鼠的致病力依次为R15056 > R15042=S15030 > Y12024=Y09011 > Y13125 > Y13164。毒力基因检测结果表明，共有3种毒力基因型，R12024、Y13164、R15042和S15030株为gdh、gapdh、fbps和orf2毒力基因阳性，Y13125和R15056株为sly、gdh、gapdh、fbps和orf2毒力基因阳性，Y09011株为gdh、fbps和orf2毒力基因阳性。药物敏感性试验结果显示，7株SS 3型对头孢喹肟相对最敏感，其次为阿莫西林、环丙沙星和磺胺间甲氧嘧啶钠，但对强力霉素100.0%耐药，且所有菌株均呈现3重以上的耐药性。本研究为进一步开展天津地区SS 3型流行特点及致病机理研究奠定了基础，可为天津地区SS 3型菌株的综合防控提供理论指导。

关键词: 猪链球菌3型; 鉴定; 致病力; 毒力基因; 药物敏感试验

Abstract: The aim of this study was to investigate the pathogenicity and drug resistance of 7 Streptococcus suis type 3 (SS 3) strains isolated from piggery in Tianjin.The pathogenicity of bacteria with doses of 1.0×10⁷,1.0×10⁸ and 1.0×10⁹ CFU/mice was studied.The virulence genes mrp,ef,sly,gdh,gapdh,fbps and orf2 of 7 strains of SS 3 strains were detected by PCR.Drug susceptibility tests of the 7 strains of SS 3 were also carried out.The results of pathogenicity test showed that 6 and 3 strains of SS 3 could cause 100.0% (5/5) morbidity and death in mice at inoculation dose of 1.0×10⁹ and 1.0×10⁸ CFU/mice,respectively.One strain of SS 3 could still cause 80.0% (4/5) morbidity and death in mice at inoculation dose of 1.0×10⁷ CFU/mice.The order of pathogenicity of the 7 strains of SS3 strains in mice was R15056 > R15042=S15030 > Y12024=Y09011 > Y13125 > Y13164.The results of virulence gene test showed that there were three virulence genotypes:R12024,Y13164,R15042 and S15030 were positive for gdh,gapdh,fbps and orf2 virulence genes;Y13125 and R15056 were positive for sly,gdh,gapdh,fbps and orf2 virulence genes,and Y09011 was positive for gdh,fbps and orf2 virulence genes.Drug susceptibility test showed that 7 SS 3 strains were relatively sensitive to cefquinoxime,followed by amoxicillin,ciprofloxacin and sulfamethoxacil sodium.However,they showed 100.0% resistance to doxycycline,and all strains showed more than 3-fold resistance.This study laid a foundation for the further development of epidemiological characteristics and pathogenic mechanism of SS 3 strains in Tianjin,and could provide theoretical guidance for the comprehensive prevention and control of SS 3 strains in Tianjin.

Key words: Streptococcus suis type 3; identification; pathogenicity; virulence gene; drug sensitivity test

中图分类号:

S852.61⁺1

池晶晶, 董志民, 田向学, 谭世旭, 李秀丽, 杨春蕾, 任卫科, 鄢明华. 天津地区猪链球菌3型菌株的致病性及耐药特性研究[J]. 中国畜牧兽医, 2019, 46(12): 3740-3748.

CHI Jingjing, DONG Zhimin, TIAN Xiangxue, TAN Shixu, LI Xiuli, YANG Chunlei, REN Weike, YAN Minghua. Study on Pathogenicity and Drug Resistance of Streptococcus suis Type 3 Strain in Tianjin[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3740-3748.

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