枸杞多糖-4a改善糖尿病大鼠胰岛素抵抗作用的研究

›› 2009, Vol. 36 ›› Issue (9): 37-41.

枸杞多糖-4a改善糖尿病大鼠胰岛素抵抗作用的研究

赵蕊¹，李青旺^2,3，张冰梅^4

(1.黑龙江八一农垦大学生命学院，大庆 163319； 2. 燕山大学环化学院，秦皇岛 066004；3.西北农林科技大学，杨凌 712100； 4.大庆油田总医院，大庆 163311)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-09-20 发布日期:2009-09-20

Study on Improving Insulin Resistance with LBP-4a in Diabetes Mellitus Rats

ZHAO Rui¹， LI Qing-wang^2,3, ZHANG Bing-mei⁴

(1.College of Life Science and Technology,Heilongjiang August First Land Reclamation University,Daqing High-Tech Industrial Development Zone, Daqing 163319, China; 2.School of Environment and Chemistry Engineering,Yanshan University, Qinhuangdao 066004, China; 3.School of Animal Science, Northwest A&F University, Yangling 712100, China; 4.Daqing Oilfield Hospital, Dqaing 163311, China)

Received:1900-01-01 Revised:1900-01-01 Online:2009-09-20 Published:2009-09-20

摘要/Abstract

摘要： 试验旨在研究枸杞多糖亚级分（LBP-4a）改善非胰岛素依赖型糖尿病(NIDDM)大鼠胰岛素抵抗（IR）的作用及其机制。试验采用水提醇沉法从宁夏枸杞中提取出枸杞多糖，并对其进行分离、纯化得主要活性成分LBP-4a。采用高糖高脂饮食结合小剂量腹腔注射链脲佐菌素(STZ)建立NIDDM大鼠模型，并将其分为模型对照组、LBP-4a低、高剂量组和罗格列酮治疗组，同时设立正常对照组。给药8周后检测相关指标。采用单细胞凝胶电泳技术对LBP-4a降糖、改善IR的作用机理进行了较为深入系统的探讨。结果表明，LBP-4a不仅对NIDDM大鼠具有降脂和增加抗氧化性的功能，还具有修复DNA损伤的作用。因此，LBP-4a作为一种可食的添加物能改善NIDDM大鼠的IR，进而预防NIDDM并发症及癌变的发生。

关键词: 枸杞多糖-4a; 非胰岛素依赖型糖尿病; 胰岛素抵抗; DNA损伤

Abstract: The experiment mean to explore the improving insulin resistance (IR) mechanism of Lycium barbarum polysaccharide-4a (LBP-4a) in non-insulin dependent diabetes mellitus (NIDDM) rats. LBP was extracted from Ningxia-Lycium barbarum by water extraction and ethanol precipitation technique. And the ingredient of LBP-4a was obtained by separation and purification. The animal model of NIDDM was made by feeding high-glucose and high-fat diet and subjecting to.i.p.little-dose streptozotocin (STZ). The rats of NIDDM model were divided into four groups, including model group, LBP-4a low-dose and high-dose groups, rosiglitazone group and normal control group. In order to research the effect of LBP-4a on reducing blood glucose and ameliorating IR in NIDDM rats, a lot of techniques were used such as Single Cell Gel Electrophoresis et al. We studied systematically the effect of LBP-4a on IR in NIDDM rats and researched the mechanism. The results indicated that LBP-4a had lipid-lowering effects on NIDDM rats. Furthermore, LBP-4a had antioxidative function and repairing DNA damage on NIDDM rats.Therefore, LBP-4a as a dietary supplement may improve IR and prevent the development of complications or even tendency to carcinogenesis in NIDDM rats.

Key words: lycium barbarum polysaccharide-4a; non-insulin dependent diabetes mellitus; insulin resistance; DNA damage

中图分类号:

R286

赵蕊;李青旺;;张冰梅. 枸杞多糖-4a改善糖尿病大鼠胰岛素抵抗作用的研究[J]. , 2009, 36(9): 37-41.

ZHAO Rui;LI Qing-wang;;ZHANG Bing-mei. Study on Improving Insulin Resistance with LBP-4a in Diabetes Mellitus Rats[J]. China Animal Husbandry & Veterinary Medicine, 2009, 36(9): 37-41.

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