高浓度过瘤胃脂肪粉对绵羊葡萄糖和胰岛素耐量的影响

doi:10.16431/j.cnki.1671-7236.2024.04.013

摘要/Abstract

摘要： 【目的】探讨高浓度过瘤胃脂肪粉(rumen protected fat,RPF)对绵羊的生长育肥性能以及葡萄糖动力学和胰岛素抵抗的影响,旨在为RPF在绵羊饲粮中的应用提供理论依据。【方法】选取体况良好的3月龄呼伦贝尔羊160只,随机分为2组,每组4个重复,每个重复20只羊,分别为CON组(不添加RPF、36.95%玉米)和HF组(23.65% RPF、16.34%玉米),预饲期为14 d,正饲期为42 d,每天记录绵羊采食量,每21 d监测1次体重变化,计算每组绵羊的平均日采食量(ADFI)、平均日增重(ADG)和料重比(F/G)。正饲期结束后,两组各选4只绵羊进行静脉注射葡萄糖耐量试验(intravenous glucose tolerance test,IVGTT),按照0.25 g/kg BW颈静脉注射50%葡萄糖溶液,采集颈静脉血液,测量血清中的葡萄糖和胰岛素浓度,计算其曲线下方正增量面积、最大浓度、最大浓度变化量及胰岛素抵抗指数(HOMA-IR和Matsuda指数)。【结果】与CON组相比,HF组的ADFI和F/G显著降低(P＜0.05)。在IVGTT期间,与CON组相比,HF组绵羊在2、10、30 min时的血清葡萄糖浓度及试验期间的葡萄糖曲线下方正增量面积均显著降低(P＜0.05),HF组的血清葡萄糖最大浓度显著低于CON组(P＜0.05);HF组在10和30 min时血清胰岛素浓度及其在60 min时曲线下方正增量面积均显著低于CON组(P＜0.05)。HF组的Matsuda指数显著高于CON组(P＜0.05)。【结论】综上所述,在生长绵羊饲粮中添加高浓度RPF不会影响绵羊的生长性能,并且可以降低绵羊血清葡萄糖浓度,提升胰岛素敏感性。

关键词: 呼伦贝尔羊; 过瘤胃脂肪粉; 葡萄糖耐量; 胰岛素抵抗

Abstract: 【Objective】 The purpose of this study was to explore the effects of high dose of rumen protected fat (RPF) on the growth performance, glucose dynamics and insulin resistance of sheep,so as to provide a scientific basis for the efficient utilization in ruminant nutrition.【Method】 A total of 160 3-months Hulunbuir sheep were selected and divided into 2 groups with 4 pens per group and 20 sheep per pen,which were named HF (23.65% RPF,16.34% corn) and CON (without RPF,36.95% corn) groups.The pre-feeding period of the feeding experiment was 14 days,and the experimental period was 42 days.The feed intake were recorded daily and the body weight of the sheep were measured every 21 days, and the average daily feed intake (ADFI), average daily gain (ADG) anf feed to gain ratio (F/G) were calculated. At the last day of the feeding period,4 sheep were randomly selected from different pens in each group,and they were subjected to intravenous glucose tolerance test (IVGTT),according to 0.25 g/kg BW,50% glucose solution was injected into the jugular vein.The serum from jugular vein blood was collected to measure the concentration of blood glucose and insulin,and the area under curve,maximum concentration,maximum concentration variation,insulin resistance index (HOMA-IR and Matsuda index) were calculated.【Result】 The ADFI and F/G of sheep in the HF group were significantly lower than those in CON group (P＜0.05).During the IVGTT,the serum glucose concentration of sheep in HF group at 2,10,30 min,and area under curve of glucose during the test were significantly lower than those in CON group (P＜0.05).The highest glucose concentration of sheep in HF group was obviously lower than that in CON group (P＜0.05).The insulin concentration at 10 and 30 min,and area under curve of insulin at 60 min in HF group were significantly lower than those in CON group (P＜0.05).The Matsuda index of HF group was obviously higher than that of CON group (P＜0.05).【Conclusion】 Adding high-concentration of RPF in the diet of growing sheep would not affect the growth performance of sheep, while could decrease the serum glucose concertration, and improve insulin sensitivity.

Key words: Hulunbuir sheep; rumen protected fat; glucose tolerance; insulin resistance

中图分类号:

S816.4

徐甜甜, 胡帆, 王雪, 伍志武, 叶炳森, 魏凌云, 贺志雄, 谭支良. 高浓度过瘤胃脂肪粉对绵羊葡萄糖和胰岛素耐量的影响[J]. 中国畜牧兽医, 2024, 51(4): 1448-1455.

XU Tiantian, HU Fan, WANG Xue, WU Zhiwu, YE Bingsen, WEI Lingyun, HE Zhixiong, TAN Zhiliang. Effect of High Concentrated of Rumen Protected Fat on Glucose and Insulin Tolerance in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1448-1455.

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