›› 2011, Vol. 38 ›› Issue (4): 183-185.

• 疾病防治 • 上一篇    下一篇

应用固相竞争ELISA、液相阻断ELISA和中和试验检测口蹄疫抗体的比较研究

廖德芳1, 李乐1, 苗海生1, 高林1, 信爱国1, 朱明旺2, 李华春1   

  1. 1. 云南省畜牧兽医科学院,云南昆明 650224;2. 云南省保山疫苗厂,云南保山 678000
  • 收稿日期:1900-01-01 修回日期:2011-01-10 出版日期:2011-04-20 发布日期:2011-04-20
  • 通讯作者: 李华春

Comparative Study between New-established FMD Antibody Identification Method SPC-ELISA and Traditional Methods LPB-ELISA and VNT

LIAO De-fang1, LI Le1, MIAO Hai-sheng1, GAO Lin1, XIN Ai-guo1, ZHU Ming-wang2, LI Hua-chun1   

  1. 1. Yunnan Animal Science and Veterinary Institute,Kunming 650224,China;2. Yunnan Baoshan Vaccine Plant,Baoshan 678000,China
  • Received:1900-01-01 Revised:2011-01-10 Online:2011-04-20 Published:2011-04-20

摘要: 为了评价建立的口蹄疫固相竞争酶联免疫吸附试验(solid phase competition ELISA,SPC-ELISA)方法与传统的中和试验(VNT)和液相阻断酶联免疫吸附试验(liquid phase block ELISA,LPB-ELISA)检测方法之间的关系,本研究对野外采集的不同种类的猪、牛和牦牛共94份血清样品分别进行O型口蹄疫病毒SPC-ELISA、LPB-ELISA和VNT检测,并分别比较3种方法的符合率和阳性检出率。结果表明,建立的O型口蹄疫病毒SPC-ELISA方法与VNT 具有很好的符合率,达88.3%,其中,猪和牦牛血清符合率高达90%和89.5%,阳性检出率为91.2%比LPB-ELISA更符合VNT,因此建立的SPC-ELISA方法比LPB-ELISA方法更符合VNT。

关键词: 口蹄疫; SPC-ELISA; LPB-ELISA; VNT; 符合率; 阳性检出率

Abstract: This study was designed to evaluate the correlation between new-established FMD antibody identification method SPC-ELISA and traditional methods LPB-ELISA and VNT. In this study,94 serum samples (10 from swine,8 from bovine and 76 from yak) which were collected from the field were used to do SPC-ELISA,LPB-ELISA and VNT to detect FMD antibody. Coincidence rate and positive rate were calculated after experiments. The result indicated that,new-established SPC-ELISA had very high coincidence rate with VNT,which was 88.3%. Swine and yak serum’s coincidence rate with VNT were even higher,respectively 90% and 89.5%. Besides,SPC-ELISA also had a higher positive rate than LPB-ELISA,which was more relevance to the positive rate of VNT. The conclusion of the analysis showed new-established FMD antibody detection method SPC-ELISA had greater superiority than traditional method LPB-ELISA.

Key words: FMD; SPC-ELISA; LPB-ELISA; VNT; coincidence rate; positive rate

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