›› 2009, Vol. 36 ›› Issue (6): 101-103.

• 遗传繁育 • 上一篇    下一篇

稀释方法对绒山羊冷冻精液品质的影响

路佩瑶1,任有蛇1,申慧敏1,岳文斌1,姚继广2,杨子森2,王慧生3,赵兴财3,白海全4   

  1. (1.山西农业大学动物科技学院, 太谷 030801; 2.山西省生态畜牧产业管理站, 太原 030001;3.岢岚县畜牧兽医局, 岢岚 036300; 4.岢岚县绒山羊种羊场, 岢岚 036300)
  • 收稿日期:1900-01-01 修回日期:2009-04-03 出版日期:2009-06-20 发布日期:2009-06-20
  • 通讯作者: 岳文斌

Effects of Dilution Methods on Frozen Semen Quality of Cashmere Goats

LU Pei-yao1,REN You-she1,SHEN Hui-min1,YUE Wen-bin1,YAO Ji-guang2,YANG Zi-sen2,WANG Hui-sheng3,ZHAO Xing-cai3,BAI Hai-quan4   

  1. (1.College of Animal Science and Technology,Shanxi Agricultural University, Taigu 030801, China; 2.Shanxi Ecological Animal Husbandry Industry Management Center, Taiyuan 030001, China;3.Kelan Animal Husbandry and Veterinary Bureau, Kelan 036300, China; 4.Kelan Cashmere Goat Breeding Farm, Kelan 036300, China)
  • Received:1900-01-01 Revised:2009-04-03 Online:2009-06-20 Published:2009-06-20
  • Contact: YUE Wen-bin

摘要: 选取7只精液品质优良的雁门绒山羊种公羊,通过测定其冷冻精液解冻后的精子活率(0、4 h)、精子存活指数、顶体完整率及精清中透明质酸酶(HYD)、谷草转氨酶(GOT)、乳酸脱氢酶(LDH)酶活力,研究一步稀释法和二步稀释法对冷冻精液品质的影响。结果表明,二步稀释法解冻后的精子存活指数显著(P<0.05)高于一步稀释法,其解冻后精清中透明质酸酶、谷草转氨酶、乳酸脱氢酶的活力均显著(P<0.05)低于一步稀释法。表明采用二步稀释法冷冻的绒山羊精液品质好于一步稀释法。

关键词: 绒山羊; 稀释方法; 冷冻精液

Abstract: Seven cashmere goats producing great semen was selected. Sperm motility(0, 4 h), sperm survival index, acrosomal integrity, enzyme activity of HYD, GOT and LDH after frozen-thawed were measured, to study the effect of one-step dilution method and two-step dilution method on semen freezing and frozen-thawed spermatozoa quality. The results showed that sperm survival index of two-step dilution method was higer significantly than that of one-step dilution method after frozen-thawed. Enzyme activity of HYD, GOT and LDH of two-step dilution method was lower significantly than those of one-step. The results showed that frozen semen quality of cashmere goats of two-step dilution method was better than that of one-step dilution method.

Key words: cashmere goats; dilution method; frozen semen

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