›› 2011, Vol. 38 ›› Issue (9): 215-218.

• 经验交流 • 上一篇    下一篇

柠檬酸、Ca2+及其联合对小鼠血清谷草转氨酶、谷丙转氨酶活性的影响

吕琼霞, 刘玉梅, 邓雯   

  1. 河南科技大学动物科技学院,河南洛阳 471003
  • 收稿日期:1900-01-01 修回日期:2011-06-26 出版日期:2011-09-20 发布日期:2011-09-20

Effects of Citric Acid,Calcium and Combination of Them on the GOT and GPT Activity of Blood in Mice

LV Qiong-xia, LIU Yu-mei, DENG Wen   

  1. Department of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China
  • Received:1900-01-01 Revised:2011-06-26 Online:2011-09-20 Published:2011-09-20

摘要： 通过测定小鼠血清谷草转氨酶(GOT)、谷丙转氨酶(GPT)的活性,探讨柠檬酸、Ca2+及二者联合对小鼠肝脏潜在毒性。将体重(20±2)g的昆明系小鼠100只,随机分为对照组,柠檬酸低、中、高剂量组,Ca2+低、中、高剂量组,柠檬酸联合Ca2+低、中、高剂量组,每组10只。分别经腹腔注射途径作相应处理,0.2 mL/只,对照组注射等量的生理盐水,注射2次,第2次注射7 d后,眼球取血,分离血清,分别测定血清中GOT和GPT的活性。结果显示,各组GOT活性均无显著差异(P>0.05)。与对照组GPT活性相比,钙高剂量组GPT活性升高51.66%,差异显著(P<0.05);钙中剂量组GPT活性升高42.63%,差异显著(P<0.05);联合高剂量组GPT活性升高35.32%,差异显著(P<0.05)。本研究结果表明,一定剂量的Ca2+能引起肝脏细胞损伤,与柠檬酸联合后,降低了其对肝脏的毒性。

关键词: 柠檬酸; Ca2+; 毒性; 谷草转氨酶; 谷丙转氨酶

Abstract: We studied the potential toxicity of citric acid, calcium and combination of them on mice by determining activity of serum of aspartate aminotransferase and alanine aminotransferase. Kunming mice with body weight of (20±2)g were randomly divided into control group, low, middle, high citric acid dose group, low, middle, high calcium dose group, low, medium and high citric acid joint calcium dose group. Every mouse was injected 0.2 mL corresponding reagents by intraperitoneal injection twice. When the seventh day after the second injection, the mice were picked eyeball for blood and determine activity of GOT and GPT in serum. The results showed that compared with the control group, the activity of GPT of high-dose calcium group increased 51.66%, which was significantly different (P<0.05);the activity of GPT of middle-dose calcium group and combined high dose group were significantly increased 42.63% (P<0.05), 35.32% (P<0.05) respectively .Compared with the control group, the activity of GOT in every group had no significantly difference. This research showed that the dose of Ca2+ can cause damage of liver cell and after combined with citric acid, liver toxicity was reduced.

Key words: citric acid; calcium; toxicity; GOT; GPT

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