关键词: VEGF基因; 荧光定量PCR; 乳腺上皮细胞; 差异表达

Abstract: In order to study the mRNA expression changes of VEGF-A,VEGF-C,and PLGF gene in different developmental periods of dairy cow mammol/lary gland,then lay the foundation for further research on the gene function of the cow mammol/lary gland. The SYBR Green Ⅰ dye Real-time PCR method was used,and the results were compared, with gene chip differential screening results of Affymetrix company. The results showed that three genes were expressed in 11 periods of mammol/lary gland. VEGF-A gene expression during late pregnancy and lactation was higher and significantly different from other periods(P<0.05);VEGF-C gene expression was higher during lactation;PLGF gene expression from the onset of virgin to pregnancy increases gradually to 4 months pregnancy the highest expression level, followed by gradually decreasing the expression. The results could be drawn from the above, VEGF-A and VEGF-C gene may play a role in the lactating cows, and PLGF gene might be play an important role to cow mammol/lary gland development, so get the PLGF CDS sequence by PCR amplified method, lay the test foundation for further research on the gene function of PLGF gene.

Key words: VEGF genes; real-time PCR; mammol/lary epithelial cells; differential expression