›› 2011, Vol. 38 ›› Issue (8): 141-146.

• 遗传繁育 • 上一篇    下一篇

军牧1号白猪、杜洛克猪和藏猪的H-FABPLPIN1基因多态性分布

印崇, 孙博兴, 赵志辉, 白春艳, 杨润军, 邢沈阳   

  1. 吉林大学畜牧兽医学院,吉林长春 130062
  • 收稿日期:2011-01-05 修回日期:1900-01-01 出版日期:2011-08-20 发布日期:2011-08-20
  • 通讯作者: 邢沈阳

The Polymorphism Distribution of H-FABP and LPIN1 Gene in Junmu 1 White,Duroc and Tibetian Pigs

YIN Chong, SUN Bo-xing, ZHAO Zhi-hui, BAI Chun-yan, YANG Run-jun, XING Shen-yang    

  1. College of Veterinary Medicine,Jilin University,Changchun 130062,China
  • Received:2011-01-05 Revised:1900-01-01 Online:2011-08-20 Published:2011-08-20

摘要: 为探索H-FABPLPIN1基因在不同猪种中的多态性分布情况,应用PCR-RFLP技术检测了61头军牧1号白猪、51头杜洛克猪和51头藏猪H-FABPLPIN1基因的多态性分布。结果发现,军牧1号白猪、杜洛克猪和藏猪在H-FABP基因的Hinf Ⅰ多态性位点上均表现为单一的HH型,而Hea Ⅲ多态性位点上均表现出多态性,等位基因D的基因频率分别为0.1475、0.2255和0.7647,Msp Ⅰ多态位点上,军牧1号白猪表现为单一的aa型,杜洛克猪和藏猪则表现为多态性,等位基因A的基因频率分别为0.6078和0.4609。在LPIN1基因的Eco88 Ⅰ多态性位点上,杜洛克猪表现为单一的AA型,军牧1号白猪和藏猪表现为多态性,等位基因A的基因频率分别为0.4262和0.2059;在Bsh1236 Ⅰ多态性位点上,杜洛克猪表现为单一的TT型,军牧1号白猪和藏猪则表现为多态性,等位基因T的基因频率分别为0.7459和0.2059。

关键词: 军牧1号白猪; 杜洛克猪; 藏猪; H-FABP基因; LPIN1基因

Abstract: In order to observe the polymorphism distribution of H-FABP and LPIN1 gene in different breeds of swines,analysis was carried out upon 61 Junmu 1 White pigs,51 Durocs pigs and 51 Tibetian pigs by PCR-RFLP. The results revealed that all these three breeds showed a single genotype of HH on the Hinf Ⅰ locus and showed polymorphism on the Hae Ⅲ locus for H-FABP,with D allelic frequency of 0.1475,0.2255 and 0.7647,respectively. The H-FABP/Msp Ⅰ locus,however,behaved a single genotype of aa in Junmu 1 White pigs and the A allelic frequency was 0.6078 and 0.4609 in Duroc and Tibetian pigs respectively. For LPIN1 /Eco88 Ⅰ,Duroc pigs showed single genotype of AA and Junmu 1 White and Tibetian pigs showed polymorphism with A allelic frequency of 0.4262 and 0.2059, respectively. For LPIN1/Bsh1236 Ⅰ locus,Duroc pigs showed single genotype of TT,Junmu 1 White and Tibetian pigs showed polymorphism with the T allelic frequency of 0.7459 and 0.2059, respectively.

Key words: Junmu 1 White pig; Duroc pig; Tibetian pig; H-FABP gene; LPIN1 gene

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