›› 2012, Vol. 39 ›› Issue (5): 90-94.

• 动物营养与饲料科学 • 上一篇    下一篇

日粮蛋白质水平对乌金猪脂肪代谢相关酶活性的影响

潘洪彬, 陈强, 黄英, 张永云, 赵素梅, 张曦, 葛长荣, 高士争   

  1. 云南农业大学云南省动物营养与饲料重点实验室, 云南昆明 650201
  • 收稿日期:2011-08-15 修回日期:1900-01-01 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 高士争

Effect of Dietary Protein Levels on Activity of Lipids Metabolic Enzymes in Wujin Pigs

PAN Hong-bin, CHEN Qiang, HUANG Ying, ZHANG Yong-yun, ZHAO Su-mei, ZHANG Xi, GE Chang-rong, GAO Shi-zheng   

  1. Yunnan Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, China
  • Received:2011-08-15 Revised:1900-01-01 Online:2012-05-20 Published:2012-05-20

摘要: 为研究日粮不同蛋白质水平对乌金猪脂肪代谢相关酶活性的影响。选取体重约15 kg的乌金猪54头,随机分为3组,每组3个重复,每个重复6头,在15~30 kg体重阶段饲喂18%(高蛋白质组)、16%(中蛋白质组)和14%(低蛋白质组)蛋白质水平的日粮,30~60 kg体重阶段饲喂16%(高蛋白质组)、14%(中蛋白质组)和12%(低蛋白质组)蛋白质水平的日粮, 60~100 kg体重阶段饲喂14%(高蛋白质组)、12%(中蛋白质组)和10%(低蛋白质组)蛋白质水平日粮,在体重30、60和100 kg体重时分批屠宰,取肝脏及背部皮下脂肪组织测定脂肪酶(LPS)、葡萄糖-6-磷酸脱氢酶(G-6-PDH)和苹果酸脱氢酶(MDH)活性。结果表明,在30 kg体重时,日粮蛋白质水平对乌金猪肝脏和脂肪组织中脂肪代谢相关酶活性差异不显着(P>0.05),在60和100 kg体重时,高蛋白质日粮组乌金猪肝脏和脂肪组织LPS的活性明显高于低蛋白质日粮组(P<0.05),G-6-PDH和MDH活性则明显低于低蛋白质日粮组(P<0.05)。因此,高蛋白质日粮增加了生长中后期乌金猪的内源性能量释放,可能影响了体脂沉积比例。

关键词: 金猪; 日粮蛋白质水平; 脂肪代谢;

Abstract: The aim of the study was to investigate the effects of dietary energy levels on lipids metabolic enzymes of liver and adipose tissue in Wujin pigs. Fifty-four Wujin pigs were randomly allotted into three groups fed diet containing different energy levels, there were eighteen pigs in each group with three replicates, six pigs were used in per replicates. Wujin pigs fed on the dietary protein levels were 18%,16%, 14% and 16%,14%, 12% and 14%,12%, 10% during the growth stages of 15 to 30,30 to 60 and 60 to 100 kg body weight, respectively, during the growth stages of 15 to 30,30 to 60 and 60 to 100 kg body weight, the dietary trace element contents in one group were constant. At the body weight of 30, 60 and 100 kg, Wujin pigs were slaughtered and liver and back subcutaneous fatty tissue were taken for analyzing contents of lipase(LPS), glucose-6 phosphate dehydrogenase (G-6-PDH) and malate dehydrogenase(MDH). The results showed that the contents of LPS,G-6-PDH and MDH in liver and back subcutaneous fatty tissue of Wujin pigs were not affected significantly by the dietary energy level at 30 kg body weight (P>0.05). At 60 and 100 kg body weight, the contents of LPS in liver and back subcutaneous fatty tissue of Wujin pigs fed on the high energy level dietary were significantly higher than the low energy level dietary(P<0.05), and, the contents of LPS,G-6-PDH and MDH in liver and back subcutaneous fatty tissue of Wujin pigs fed on the high energy level dietary were significantly lower than the low energy level dietary (P<0.05). The results indicated that higher energy level of dietary could increase endogenous energy release, body fat deposition ratio influenced in Wujin pigs at growth midanaphase.

Key words: Wujin pigs; dietary energy level; fat metabolism; enzyme

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