GPx5在正常及隐睾症双峰驼睾丸及附睾中的作用分析

doi:10.16431/j.cnki.1671-7236.2025.03.020

摘要/Abstract

摘要： 【目的】分析谷胱甘肽过氧化物酶-5(glutathione peroxidase-5，GPx5)在正常及隐睾症双峰驼睾丸及附睾中的分布与表达，探索其在双峰驼隐睾时精子的抗氧化对生殖的调控作用。【方法】选取成年(5岁)双峰驼正常睾丸附睾10对及隐睾睾丸附睾6对，利用HE染色、胶原染色、网状染色观察其组织学结构特征，通过免疫组织化学染色、Western blotting、免疫荧光染色结合Image Pro Plus 13.0图像分析研究GPx5的表达及分布。【结果】与正常组相比，隐睾组双峰驼生精小管发育不良且管腔直径极显著缩小(P＜0.01)，间质细胞减少，附睾管腔缩小且上皮形成空泡，睾丸及附睾间质网状纤维和胶原纤维较丰富。免疫组织化学结果显示，GPx5在正常组双峰驼睾丸中未见明显表达，在隐睾组支持细胞中可见明显表达。Western blotting结果显示，与正常组相比，GPx5在隐睾组双峰驼睾丸和附睾体中表达量极显著升高(P＜0.01)，在附睾头中表达量极显著降低(P＜0.01)，在附睾尾中表达量无显著差异(P＞0.05)。免疫荧光定位显示，与正常组相比，GPx5在隐睾组双峰驼睾丸和附睾尾中表达量极显著升高(P＜0.01)，在附睾头中表达量极显著降低(P＜0.01)，在附睾体中表达量无显著差异(P＞0.05)。【结论】双峰驼隐睾的睾丸及附睾趋向纤维化；GPx5蛋白表达量在双峰驼隐睾睾丸中显著增加，且在支持细胞中表达增强，生精小管中的氧化应激影响了精子的正常生成；在附睾头的主细胞表达微弱，提示附睾微环境的抗氧化异常，精子损伤明显。

关键词: 谷胱甘肽过氧化物酶-5(GPx5); 双峰驼; 隐睾; 睾丸; 附睾

Abstract: 【Objective】 This study was aimed to analyze the distribution and expression of glutathione peroxidase-5 (GPx5) in testis and epididymis of normal and cryptorchidism in Bactrian camels,so as to explore the regulatory effect on reproduction of GPx5 in sperm antioxidant function during cryptorchidism in Bactrian camels.【Method】 The testis and epididymis from adult (5-year-old) Bactrian camels were collected,including 10 pairs of normal group and 6 pairs of cryptorchid group,the histological structural characteristics were observed using HE staining,collagen staining and reticular staining.The expression and distribution of GPx5 were studied by immunohistochemistry staining,Western blotting,immunofluorescence staining,and Image Pro Plus 13.0 image analysis.【Result】 Compared with normal group,Bactrian camels in cryptorchid group showed underdeveloped seminiferous tubules with extremely significantly reduced lumen diameter (P＜0.01),fewer interstitial cells,shrunken lumen with epithelial vacuolation in epididymise,but more abundant reticular and collagen fibers of interstitium in testis and epididymis.The immunohistochemistry results revealed that there was no obvious expression of GPx5 in testis of Bactrian camels in normal group,while there was evident expression in Sertoli cells of Bactrian camels in cryptorchid group.Western blotting results showed that compared with normal group,the expression of GPx5 in testis and corpus epididymidis of Bactrian camels in cryptorchid group was extremely significantly increased (P＜0.01),the expression of GPx5 in caput epididymidis was extremely significantly decreased (P＜0.01),but there was no significant difference in cauda epididymidis (P＞0.05).The results of immunofluorescence localization indicated that compared with normal group,the expression of GPx5 in testis and cauda epididymidis of Bactrian camels in cryptorchid group was extremely significantly increased (P＜0.01),the expression of GPx5 in caput epididymidis was extremely significantly decreased (P＜0.01),but there was no significant difference in corpus epididymidis (P＞0.05).【Conclusion】 The testis and epididymis of cryptorchidism in Bactrian camels showed a tendency towards fibrosis.The expression of GPx5 protein was significantly increased in the testis of cryptorchidism in Bactrian camels,with enhanced expression in Sertoli cells.The oxidative stress in seminiferous tubules affected normal spermatogenesis.The weak expression of GPx5 in the principal cells of caput epididymidis suggested an abnormal antioxidant activity in the epididymal microenvironment,leading to obvious sperm damage.

Key words: glutathione peroxidase-5 (GPx5); Bactrian camels; cryptorchidism; testis; epididymis

中图分类号:

S824

路亚男, 袁莉刚, 曾建林, 齐玉梅, 马龙, 吕金瀚. GPx5在正常及隐睾症双峰驼睾丸及附睾中的作用分析[J]. 中国畜牧兽医, 2025, 52(3): 1180-1190.

LU Yanan, YUAN Ligang, ZENG Jianlin, QI Yumei, MA Long, LYU Jinhan. Analysis of the Effect of GPx5 on Testis and Epididymis of Normal and Cryptorchidism in Bactrian Camels[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1180-1190.

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