青蒿散抗鸡球虫药效研究

doi:10.16431/j.cnki.1671-7236.2018.06.033

摘要/Abstract

摘要：

为探讨青蒿散对鸡柔嫩艾美耳球虫（E.tenella）的防治效果，本试验选取90只健康黄羽肉鸡，分为6组，除空白对照组外，其余各组（感染对照组、药物对照组及青蒿散低、中、高剂量组）试验鸡均经口感染6×10⁴个柔嫩艾美耳球虫孢子化卵囊，各试验组在接种前一天开始用药至试验结束。记录各组试验鸡的眼观病变、组织病理学检查、相对增重率、盲肠病变记分、血便记分、每克粪便中的卵囊数（OPG）、卵囊值及抗球虫指数（ACI）指标，评价青蒿散不同给药浓度的抗球虫效果。结果显示，空白对照组、感染对照组、药物对照组及青蒿散低、中、高剂量组的抗球虫指数分别为200.00、72.23、157.19、82.89、125.32和137.06，与感染对照组相比，药物对照组和青蒿散各剂量组的眼观病变、组织病理学检查均有所缓解，青蒿散能减轻病鸡盲肠的肿胀和出血等症状，且病鸡精神状态较感染对照组好；与感染对照组相比，各给药组的平均增重均有所升高，盲肠病变、卵囊值及血便情况减少。综上所述，青蒿散能缓解柔嫩艾美耳球虫侵染鸡盲肠所致的病理症状和组织病变，减少粪便中卵囊的排出数量，有一定的抗球虫效果，且给药浓度越高抗球虫效果越好，具有剂量依赖性。

关键词: 青蒿散; 柔嫩艾美耳球虫; 抗鸡球虫; 抗球虫指数

Abstract:

In order to investigate the control effect of Artemisia annua powders on chicken coccidian,90 healthy yellow feather broilers were selected and divided into 6 groups.Except the blank control group,other chickens were infected with Eimeria tenella (E.tenella),with 6×10⁴ sporulated oocysts,and each test group began to use drugs on the day before the vaccination,until to the end of the test.By recording the indexs of each test group,including their macroscopical lesion,histopathological examination,relative weight-growth rate,lesion scores of pathological changes of cecum,lesion scores of bloody stool,the number of oocysts (OPG),per gram of feces,oocyst score and anticoccidial index (ACI),the thesis made attempts to evaluate anticoccidia effects.The results revealed that the ACI of blank control group,infection control group,drug control group and low,middle and high dose groups of Artemisia annua powders were 200.00,72.23,157.19,82.89,125.32 and 137.06,respectively.Compared with infection control group,the macroscopical lesion,histopathological examination of drug control group and drug supplied groups of Artemisia annua powders were relieved.Artemisia annua powders could reduce sick chicken's cecum swelling and bleeding and other symptoms,and they were in much better spirits than infection control group.Compared with the infection control group,the average weight gain of each drug supply group were all increased,and their cecal lesion scores,oocyst score and bloody stools decreased.The results showed that Artemisia annua powders could alleviate pathological symptoms and tissue lesions infected by Eimeria tenella of chicken cecum,and reduce the amount of feces in the oocysts.The higher the concentration of administration,the better the anticoccidial effect,which was dose-dependent.

Key words: Artemisia annua powder; Eimeria tenella; anti-chicken coccidian; anti-coccidia index

中图分类号:

S853.7

牛彪, 秦文文, 郭文柱, 张璞俊, 马莉平, 黄鑫, 刘宇, 梁剑平. 青蒿散抗鸡球虫药效研究[J]. 《中国畜牧兽医》, 2018, 45(6): 1683-1691.

NIU Biao, QIN Wenwen, GUO Wenzhu, ZHANG Pujun, MA Liping, HUANG Xin, LIU Yu, LIANG Jianping. Study on Anticoccidial Efficacy of Artemisia annua Powder[J]. , 2018, 45(6): 1683-1691.

参考文献

[1] 宋培勇,曾燕玲,张仁波.鸡球虫病及其防治[J].养禽与禽病防治,2010,6:33-35. SONG P Y,ZENG Y L,ZHANG R B.Prevention and cure of chicken coccidiosis[J].Poultry Husbandry and Disease Control,2010,6:33-35.(in Chinese)
[2] WILLIAMS R B.Epidemiological studies of coccidiosis in the domesticated fowl (Gallus gallus):Ⅰ.The fate of ingested oocysts of Eimeria tenella during the prepatent period in susceptible chicks[J].Applied Parasitology,1995,36(2):90-96.
[3] CHAPMAN H D.Biochemical,genetic and applied aspects of drug resistance in Eimeria parasites of the fowl[J]. Avian Pathology,1997,26(2):221-244.
[4] 付文涛,周变华,黄婷婷,等.中药有效成分抗鸡球虫病研究进展[J].动物医学进展,2016,37(6):98-101. FU W T,ZHOU B H,HUANG T T,et al.Progress on several kinds of traditional chinese medicines in control of chicken coccidiosis[J]. Progress in Veterinary Medicine,2016,37(6):98-101.(in Chinese)
[5] TEWARI A K,MAHARANA B R.Control of poultry coccidiosis:Changing trends[J].Journal of Parasitic Diseases,2011,35(1):10-17.
[6] ZAMAN M A,IQBAL Z,ABBAS R Z,et al.Anticoccidial activity of herbal complex in broiler chickens challenged with Eimeria tenella[J].Parasitology,2012,139(2):237-243.
[7] DALLOUL R A,LILLEHOJ H S.Poultry coccidiosis:Recent advancements in control measures and vaccine development[J]. Expert Review of Vaccines,2006,5(1):143-163.
[8] 刘春朝,王玉春,欧阳藩,等.青蒿素研究进展[J].化学进展,1999,11(1):41-48. LIU C C,WANG Y C,OU Y F,et al.Advance in artemisinin research[J]. Progress in Chemistry,1999,11(1):41-48.(in Chinese)
[9] ALLEN P C,LYDON J,DANFORTH H D.Effects of components of Artemisia annua on coccidia infections in chickens[J].Poultry Science,1997,76(8):1156-1163.
[10] 吴阳阳,王安源,张醒醒,等.青篙素、黄花篙与黄连素单独和联用的抗球虫作用研究[J].中兽医学杂志,2016,3:4-6. WU Y Y,WANG A Y,ZHANG X X,et al.Study on anti-coccidiosis effects of artemisinin,artemisin and berberine alone and in combination[J]. Chinese Journal of Traditional Veterinary Science,2016,3:4-6.(in Chinese)
[11] DEL C E,PAGÉS M,GALLEGO M,et al.Meiotic chromosome pairing and bouquet formation during Eimeria tenella sporulation[J].International Journal for Parasitology, 2010,40(4):453-462.
[12] 宋萍,马庆涛,苏洋洋,等.青蒿素类药物对鸡柔嫩艾美耳球虫感染的防治试验[J].中国家禽,2014,36(5):40-41. SONG P,MA Q T,SU Y Y,et al.Artemisinin drugs on prevention and treatment of Eimeria tenella infection in chickens[J].China Poultry,2014,36(5):40-41.(in Chinese)
[13] 黄鑫,郭文柱,高旭东,等.青蒿素的来源及其抗鸡球虫作用机制研究进展[J].中国预防兽医学报,2016,38(6):503-506. HUANG X,GUO W Z,GAO X D,et al.The source of artemisinin and its anti-coccidia mechanism of action[J].Chinese Journal of Preventive Veterinary Medicine,2016,38(6):503-506.(in Chinese)
[14] DEL C E,GALLEGO M,FRANCESCH M,et al.Effect of artemisinin on oocyst wall formation and sporulation during Eimeria tenella infection[J].Parasitology International,2010,59(4):506-511.
[15] 马琰,马庆涛,承南,等.青蒿素与抗菌增效剂联合抗鸡球虫效果评价[J].中国家禽,2016,38(2):53-55. MA Y,MA Q T,CHENG N,et al.Evaluation of the effect of artemisinin and antibacterial synergist against chicken coccidia[J].China Poultry,2016,38(2):53-55.(in Chinese)
[16] MOREHOUSE N F,BARON R R.Coccidiosis:Evaluation of coccidiostats by mortality,weight gains,and fecal scores[J].Experimental Parasitology,1970,28(1):25-29.
[17] JOHNSON J,REID W M.Anticoccidial drugs:Lesion scoring techniques in battery and floor-pen experiments with chickens[J].Experimental Parasitology, 1970,28(1):30-36.
[18] 角田清.鸡球虫病[M].上海:上海科学技术文献出版社,1986. JIAO T Q.Chicken Coccidiosis[M].Shanghai:Shanghai Science and Technology Literature Publishing House,1986.(in Chinese)
[19] 郑姣妹,罗冬生,刘毅.青蒿素及其衍生物抗寄生虫的研究进展[J].中国动物传染病学报,2005,13(3):38-41. ZHENG J M,LUO D S,LIU Y.Progress of research on the artemisinin and it's derivation against parasitism[J].Chinese Journal of Veterinary Parasitology,2005,13(3):38-41.(in Chinese)
[20] 张会中,张润轮.鸡球虫的耐药性与应对措施[J].养禽与禽病防治,2010,3:29-31. ZHANG H Z,ZHANG R L.Resistance of chicken coccidiosis and countermeasures[J].Poultry Husbandry and Disease Control,2010,3:29-31.(in Chinese)
[21] 周翠珍,齐雪茹,王红梅,等.纯中草药散剂对鸡球虫病的疗效试验[J].中国畜牧兽医,2007,34(7):89-90. ZHOU C Z,QI X R,WANG H M,et al.Efficacy of pure Chinese herbal powder on chicken coccidiosis[J].China Animal Husbandry & Veterinary Medicine,2007,34(7):89-90.(in Chinese)
[22] 刘佳,郭莉,刘建伟.中药复方对鸡柔嫩艾美尔球虫病的疗效观察[J].动物医学进展,2010,31(s1):175-178. LIU J,GUO L,LIU J W.Therapeutic effect of Chinese herbal medicine prescription on Eimeria tenella in chickens[J]. Progress in Veterinary Medicine,2010,31(s1):175-178.(in Chinese)
[23] 郭红斌,弓素梅,王凤霞.四个抗球虫中药复方对肉鸡球虫病疗效对比试验[J].湖北农业科学,2011,50(13):2702-2705. GUO H B,GONG S M,WANG F X.Efficacy comparative of four Chinese herbal compounds on coccidiosis in broiler chickens[J].Hubei Agricultural Sciences,2011,50(13):2702-2705.(in Chinese)
[24] 黄占欣,米同国,赵达,等.中药抗球散防治鸡球虫病的试验[J].中国兽医杂志,2016,52(3):71-73. HUANG Z X,MI T G,ZHAO D,et al.Experiment of Chinese herbal revention and control of coccidiosis[J].Chinese Journal of Veterinary Medicine,2016,52(3):71-73.
[25] 李宏娟,刘聚祥,刘静.中药复方对鸡柔嫩艾美尔球虫病的疗效观察[J].华北农学报,2008,23(增刊):292-296. LI H J,LIU J X,LIU J.Therapeutic effect of Chinese herbal medicine prescription on Eimeria tenella in chickens[J].Acta Agriculturae Boreali-Sinica,2008,23(Suppl):292-296.(in Chinese)
[26] 莫平华,马庆涛,纪小霞,等.青蒿素对鸡柔嫩艾美耳球虫第二代裂殖子微线基因mRNA转录及鸡盲肠组织结构的影响[J].畜牧兽医学报,2014,45(5):833-838. MO P H,MA Q T,JI X X,et al.Effects of artemisinin treatment on microneme genes transcription in second-generation merozoites and pathological changes of caecumin chickens infected by Eimeria tenella[J].Acta Veterinaria et Zootechnica Sinica,2014,45(5):833-838.(in Chinese)
[27] 罗丹,刘伟光,杨亚明.青蒿素类抗疟药的作用机制及耐药机制研究进展[J].中国医学创新,2014,11(9):131-134. LUO D,LIU W G,YANG Y M.Research progress in antimalarial mechanism and drug-resistant mechanism of artemisinin and its derivatives[J]. Medical Innovation of China,2014,11(9):131-134.(in Chinese)
[28] 张婷,赵旭,桑晓宇,等.青蒿素及其衍生物抗寄生虫药理作用研究进展[J].动物医学进展,2017,38(10):98-102. ZHANG T,ZHAO X,SANG X Y,et al.Advances in pharmacological effects of artemisinin and its derivatives against parasites[J].Progress in Veterinary Medicine,2017,38(10):98-102.(in Chinese)