1,25-二羟维生素D3对肉鸡生长性能、骨骼矿化及小肠NaPi-Ⅱb和VDR mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2018.10.008

摘要/Abstract

摘要：

试验旨在研究不同水平1，25-二羟基维生素D₃（1，25-（OH）₂-D₃）对1~14日龄肉鸡生长性能、骨骼矿化及促进十二指肠磷吸收关键基因表达的影响。将180只1日龄罗斯308肉鸡分入3个处理，每处理6个重复，每重复10只。3个处理组分别饲喂3种日粮：在基础日粮中分别添加0、5、10 μg/kg 1，25-（OH）₂-D₃，试验期14 d。14日龄屠宰肉鸡，剥离股骨、胫骨及十二指肠1/2处的黏膜；测定肉鸡的生长性能、骨骼矿化相关指标，分析Ⅱb型磷酸钠转运蛋白（NaPi-Ⅱb）、细胞核维生素D受体（nVDR）和细胞膜维生素D受体（mVDR）基因表达情况。结果显示：与基础日粮组相比，添加5 μg/kg 1，25-（OH）₂-D₃组肉鸡体增重、采食量、料重比、股骨和胫骨各指标（重量、长度、直径、灰分重量、灰分含量、钙、磷含量）及十二指肠NaPi-Ⅱb、nVDR和mVDR mRNA表达量均未发生显著变化（P>0.05）；添加10 μg/kg 1，25-（OH）₂-D₃显著降低了肉鸡体增重、采食量、股骨和胫骨各指标及十二指肠NaPi-Ⅱb、nVDR和mVDR mRNA表达量（P<0.05），同时料重比显著增加（P<0.05）。综合上述结果，在基础日粮中添加5 μg/kg 1，25-（OH）₂-D₃不会影响1~14日龄肉鸡的生长、骨骼矿化及十二指肠磷吸收关键基因表达；但添加量增至10 μg/kg会显著抑制1~14日龄肉鸡生长、骨骼发育，影响十二指肠NaPi-Ⅱb和VDR mRNA表达。

关键词: 1; 25-(OH)₂-D₃; 肉鸡; 生长性能; 骨骼矿化; NaPi-Ⅱb; nVDR; mVDR

Abstract:

The experiment was aimed to study the effects of 1,25-dihydroxycholecalciferol (1,25-(OH)₂-D₃) on growth performance,bone mineralization and duodenal phosphate transport gene expression.A total of 180 broilers (1 day old)were divided into 3 treatments with 6 replicates per treatment of 10 chickens per replicate.Three levels of 1,25-(OH)₂-D₃ were added to the basic diet:0,5 and 10 μg/kg,respectively.Broilers were slaughtered at 14 days of age,and femur,tibia,and duodenal mucosa were collected.Growth performance,bone mineralization and mRNA expressions of sodium phosphate transporter (NaPi-Ⅱb),nuclear vitamin D receptor (nVDR) and membrane vitamin D receptor (mVDR) were analyzed.The results showed that:Compared with the basic group,addition 5 μg/kg 1,25-(OH)₂-D₃ did not significantly affect the body weight gain,feed intake,feed and gain ration,and the weight,length,diameter,ash weight,ash content,calcium content,and phosphorus content of femur and tibia,and mRNA expression of NaPi-Ⅱb,nVDR and mVDR in the duodenum of broilers from 1 to 14 days of age (P > 0.05).Adding 10 μg/kg 1,25-(OH)₂-D₃ significantly reduced body weight gain,feed intake and feed conversion,the quality of femur and tibia,and the mRNA expression of NaPi-Ⅱb,nVDR,and mVDR in the duodenum of broilers (P < 0.05).In conclusion,adding 5 μg/kg 1,25-(OH)₂-D₃ to normal diets did not affect the growth,bone mineralization or the mRNA expression of NaPi-Ⅱb,nVDR and mVDR in duodenum of broilers.However,the addition of 10 μg/kg 1,25-(OH)₂-D₃ reduced the growth performance,skeletal mineralization and inhibits duodenal NaPi-Ⅱb,nVDR and mVDR gene expression at the transcriptional level.

Key words: 1,25-(OH)₂-D₃; broiler chicken; growth performance; bone mineralization; NaPi-Ⅱb; nVDR; mVDR

中图分类号:

S816.71

张宁, 杨雪, 张金龙, 王志祥, 韩进诚, 卢红梅, 陈村年. 1,25-二羟维生素D₃对肉鸡生长性能、骨骼矿化及小肠NaPi-Ⅱb和VDR mRNA表达的影响[J]. 《中国畜牧兽医》, 2018, 45(10): 2716-2723.

ZHANG Ning, YANG Xue, ZHANG Jinlong, WANG Zhixiang, HAN Jincheng, LU Hongmei, CHEN Cunnian. Effects of 1, 25-dihydroxycholecalciferol on Growth Performance, Bone Mineralization and mRNA Expression of NaPi-Ⅱb and VDR in Small Intestine of Broiler[J]. , 2018, 45(10): 2716-2723.

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1,25-二羟维生素D₃对肉鸡生长性能、骨骼矿化及小肠NaPi-Ⅱb和VDR mRNA表达的影响

Effects of 1, 25-dihydroxycholecalciferol on Growth Performance, Bone Mineralization and mRNA Expression of NaPi-Ⅱb and VDR in Small Intestine of Broiler

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