猪圆环病毒2型基因组DNA的结构与功能研究进展

doi:10.16431/j.cnki.1671-7236.2016.05.008

摘要/Abstract

摘要： 猪圆环病毒2型(PCV2)是引起猪圆环病毒病相关疾病的主要致病原,可损伤宿主的免疫系统,加剧一些细菌病和病毒病感染的临床症状,在全球养猪业中造成巨大损失。PCV2的基因组DNA含有11个开放阅读框(ORFs),其中至少有7个ORFs编码的蛋白质分子质量大于5ku,但目前只有5种编码的蛋白(Rep、Rep'、Cap、ORF3和ORF4)发现于PCV2复制过程中。作者概述了当前PCV2基因组DNA的结构与功能,阐明PCV2的病原学,为防控PCV2感染提供科学依据。

关键词: 猪圆环病毒2型; 基因组DNA; 开放阅读框; 病毒编码蛋白

Abstract: Porcine circovirus type 2 (PCV2) is a disease caused by porcine circovirus, which can damage the host's immune system and exacerbate the clinical symptoms of many bacterial and viral infections.It has caused increasingly larger losses in the pig industry wordwide.The genomic DNA of PCV2 contains 11 open reading frames (ORFs) and at least seven potential ORFs-encoding proteins larger than 5 ku.However, only five virally encoded proteins, including Rep, Rep', Cap, ORF3 and ORF4, had been identified in PCV2 replication.In this review, we discussed the progress on structure and function of genomic DNA of PCV2 in order to provide insight into the scientific basis of the pathogenesis of PCV2 and prevent its infection.

Key words: porcine circovirus type 2; genomic DNA; open reading frames; virally encoded proteins

中图分类号:

S852.65

赵燕, 喻正军. 猪圆环病毒2型基因组DNA的结构与功能研究进展[J]. , 2016, 43(5): 1176-1181.

ZHAO Yan, YU Zheng-jun. Advances on Structure and Function of Genomic DNA of Porcine Circovirus Type 2[J]. , 2016, 43(5): 1176-1181.

参考文献

[1] Segales J,Allan G M,Domingo M.Porcine circovirus disease[J].Animal Health Research Reviews,2005,6(2):119-142.
[2] 王宪文,姚四新,王丽荣,等.猪圆环病毒Ⅱ型流行病学新特点及致病机理研究进展[J].中国畜牧兽医,2012,39(12):190-194.
[3] 李任峰,陈金山,赵坤,等.河南豫北地区猪"高热病"流行特征及病原学研究[J].广东农业科学,2011,38(15):92-95.
[4] Tischer I,Gelderblom H,Vettermann W,et al.A very small porcine virus with circular single-stranded DNA[J].Nature,1982,295:64-66.
[5] 乔翠,高凤山,许崇波.猪圆环病毒2型分子致病机理及相关疾病研究进展[J].中国畜牧兽医,2012,39(2):170-173.
[6] Hamel A L,Lin L L,Nayar G P.Phylogenetic and recombination analysis of genomic sequences of PCV2 isolated in Korea[J].Virology Journal,1998,72:5262-5267.
[7] Cheung A K.Characterization of a previously unidentified viral protein in PCV2-3[J].Virology Journal,2003,305:168-180.
[8] Darwich L,Segales J,Mateu E.Porcine circovirus type 2 and porcine circovirus-associated disease[J].Archives of Virology,2004,149(5):857-874.
[9] Finsterbusch T,Mankertz A.Porcine circovirus type 2 in China:An update on and insights to its prevalence and control[J].Virus Research,2009,143(2):177-183.
[10] Mankertz A,Mueller B,Steinfeldt T,et al.Functional exchangeability of the nuclear localization signal (NLS) of capsid protein between PCV1 and PCV2 in vitro:Implications for the role of NLS in viral replication[J].Virology Journal,2003,77(18):9885-9893.
[11] Mankertz A,Hillenbrand B.Correlation of the cyclin A expression level with porcine circovirus type 2 propagation efficiency[J].Virology Journal,2001,279:429-438.
[12] Nawagitgul P,Morozov I,Bolin S R,et al.Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein[J].Virology Journal,2000,81:2281-2287.
[13] Misinzo G,Delputte P L,Meerts P,et al.Open access co-existence of multiple strains of porcine circovirus type 2 in the same pig from China[J].Virology Journal,2006,80(7):3487-3494.
[14] O'Dea M A,Hughes A P,Davies L J,et al.The detection of porcine circovirus in the Australian pig herd[J].Journal of Virology Methods,2008,147(1):61-66.
[15] Fenaux M,Opriessnig T,Halbur P J,et al.Phylogenetic analysis of porcine circovirus type 2 (PCV2) pre- and post-epizootic postweaning multisystemic wasting syndrome (PMWS)[J].Virology Journal,2004,78:13440-13446.
[16] Meehan B M,McNeilly F,Todd D,et al.A recombinant porcine circovirus type 2 expressing the VP1 epitope of the type O foot-and-mouth disease virus is infectious and induce both PCV2 and VP1 epitope antibodies[J].Journal of General Virology,1998,79:2171-2179.
[17] Dong B,Feng J,Hai L,et al.Immune responses of mice immunized by DNA plasmids encoding PCV2 ORF2 gene,porcine IL-15 or the both[J].Vaccine,2013,31(48):5736-5744.
[18] Gilpin D F,McCullough K,Meehan B M,et al.Functional analysis of the interferon-stimulated response element of porcine circovirus type 2 and its role during viral replication in vitro and in vivo[J].Veterinary Immunology Immunopathology,2003,94:149-161.
[19] Liu J,Chen I,Kwang J.Characterization of porcine circovirus type 2 (PCV2) capsid particle assembly and its application to virus-like particle vaccine development[J].Virology Journal,2005,79:8262-8274.
[20] Liu J,Chen I,Du Q,et al.Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus[J].Virology Journal,2006,80:5065-5073.
[21] Karuppannan A K,Jong M H,Lee S H,et al.Porcine circovirus 2:Immunopathogenesis and recent developments in vaccines[J].Virology Journal,2009,383:338-347.
[22] Timmusk S,Merlot E,Lovgren T,et al.Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification[J].Virology Journal,2009,90:2425-2436.
[23] Karuppannan A K,Liu S,Jia Q,et al.Porcine circovirus type 2 ORF3 protein competes with p53 in binding to Pirh2 and mediates the deregulation of p53 homeostasis[J].Virology Journal,2010,398:1-11.
[24] Gao Z Z,Dong Q F,Jiang Y G,et al.Porcine circovirus type 2 and porcine circovirus-associated disease[J].Virus Genes,2013,47(2):268-275.
[25] Gao Z Z,Dong Q F,Jiang Y H,et al.ORF4-protein deficient PCV2 mutants enhance virus-induced apoptosis and show differential expression of mRNAsin vitro[J].Virus Research,2014,183:56-62.
[26] Olvera A,Cortey M,Segales J.Genetic variation and phylogenetic analysis of porcine circovirus type 2 infections in central China[J].Virology Journal,2007,357:175-185.
[27] Gao Z Z,Dong Q F,Jiang Y H,et al.Alternative trans-splicing:A novel mode of pre-mRNA processing[J].Virus Research,2014,183:56-62.
[28] Meng X J.First analysis of synonymous codon usage in porcine circovirus[J].Annu Rev Anim Biosci,2013,1:43-64.
[29] Nauwynck H J,Sanchez R,Meerts P,et al.Recent advances in porcine circoviruses and associated diseases[J].Virus Research,2012,164:43-45.
[30] Fenaux M,Opriessnig T,Halbur P G,et al.A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs[J].Virology Journal,2004,78:13440-13446.
[31] Ramamoorthy S,Opriessnig T,Pal N,et al.The effect of infection order of porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus on dually infected swine alveolar macrophages[J].Veterinary Microbiology,2011,147(1-2):49-58.
[32] Meehan B M,Creelan J L,McNulty M S,et al.Cloning and sequence analysis of two banana bunchy top virus genomes in Hainan[J].Journal of General Virology,1997,78:221-227.
[33] Galluzzi L,Kepp O,Morselli E,et al.Viral strategies for the evasion of immunogenic cell death[J].Internal Medicine,2010,267:526-542.
[34] Galluzzi L,Brenner C,Morselli E,et al.A fine balance between life and death:Mulation of BCL-2 family members by Toxoplasma gondii[J].PLoS Pathogens,2008,4(5):e1000018.
[35] Wei L,Zhu S S,Wang J,et al.Inhibition of the PI3K/Akt pathway by Ly294002 does not prevent establishment of persistent Junín virus infection in Vero cells[J].Virology Journal,2012,86(24):13589-13597.
[36] Caroline F,Bernt H,Tanja L,et al.PCV2 on the spot- A new method for the detection of single porcine circovirus type 2 secreting cells[J].Journal of Virological Methods,2014,196:185-192.
[37] Gerald R,Regina H,Hermann W.Genetic variability of porcine circovirus 2 (PCV2) field isolates from vaccinated and non-vaccinated pig herds in Germany[J].Veterinary Microbiology,2015,180(1-2):41-48.
[38] Li P C,Qiao X W,Zheng Q S,et al.Immunogenicity and immunoprotection of porcine circovirus type 2 (PCV2) cap protein displayed by lactococcus lactis[J].Vaccine,2016,34(5):696-702.
[39] Wang W C,Zeng Z Y,Tang D Y,et al.Construction of a novel porcine circovirus type 2 infectious clone as a basis for the development of a PCV2 iDNA vaccine[J].Journal of Virological Methods,2015,220:21-26.
[40] Giovanni F,Marti C,Ubiratan P,et al.Genetic characterisation of porcine circovirus type 2 (PCV2) strains from feral pigs in the Brazilian Pantanal:An opportunity to reconstruct the history of PCV2 evolution[J].Veterinary Microbiology,2015,178(1-2):158-162.