中国畜牧兽医

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1株羊肺源金黄色葡萄球菌的分离鉴定及耐药性分析

裴志花,孙小宁,王开   

  1. (吉林农业大学动物科学技术学院,吉林长春  130118

  • 收稿日期:2013-11-28 出版日期:2014-06-20 发布日期:2014-07-24
  • 通讯作者: 王开。E-mail: wk197811@126.com
  • 作者简介:裴志花(1978—),女,内蒙古人,硕士,实验师,研究方向:动物药理学与生理学。
  • 基金资助:

    吉林省科技厅青年基金项目(201201099);吉林省教育厅项目(2014-24)。

Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus from Sheep Lungs

PEI Zhi-hua, SUN Xiao-ning, WANG Kai   

  1. (College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

  • Received:2013-11-28 Online:2014-06-20 Published:2014-07-24

摘要: 采用16S rRNA序列分析及生化分析的方法,从某规模育肥羊场的患呼吸系统疾患的病羊分离到1株金黄色葡萄球菌,命名为JLY-1。药敏试验结果显示,JLY-1是1株多重耐药葡萄球菌,对红霉素、氨苄西林、复方新诺明高度耐药(MIC≥512 μg/mL);对多西环素、丁胺卡那(MIC≥64 μg/mL)和环丙沙星、恩诺沙星(MIC≥4 μg/mL)耐药;对氟苯尼考和头孢曲松敏感(MIC≤0.25 μg/mL)。

关键词:

羊; 金黄色葡萄球菌; 分离鉴定; 耐药性

Abstract: One strain of Staphylococcus aureus was isolated and identified by 16S rRNA sequence analysis and biochemical analysis in a large-scale sheep farms, it was named as JLY-1. The drug resistance analysis of the strain was conducted. The result showed that JLY-1 was highly resistant to erythromycin, ampicillin, chemitrim (MIC≥512 μg/mL);resistant to doxycycline, amikacin (MIC≥64 μg/mL) and ciprofloxacin, enrofloxacin (MIC≥4 μg/mL);and sensitive to florfenicol and cefatriaxone (MIC≤0.25 μg/mL).

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