藏猪促红细胞生成素的表达与低氧适应分析

中国畜牧兽医

藏猪促红细胞生成素的表达与低氧适应分析

吴洪¹，德吉巴卓¹，张健¹，王亚非¹，张浩²，强巴央宗¹

（1.西藏大学农牧学院动物科学学院，西藏林芝 860000；2.中国农业大学动物科技学院，北京 100193）

收稿日期:2013-12-04 出版日期:2014-04-20 发布日期:2014-05-27
通讯作者: 强巴央宗（1965—），女，西藏人，博士，教授，研究方向：动物分子数量遗传学与资源保存利用。E-mail:qbyz628@126.com；Tel：0894-5822924
作者简介:吴洪（1989—），男，四川人，硕士，研究方向：病毒分子生物学。
基金资助:
国家自然科学基金（31160441）。

EPO Protein Expression and Hypoxic Adaptation Analysis in Tibetan Pig

WU Hong¹,DEJI Bazhuo¹,ZHANG Jian¹,WANG Ya-fei¹,ZHANG Hao²,CHAMBA Yangzom¹

(1.College of Animal Science, College of Agriculture and Animal Husbandry, Tibet University,Linzhi 860000，China；2.College of Animal Science and Technology, China Agricultural University, Beijing 100193,China)

Received:2013-12-04 Online:2014-04-20 Published:2014-05-27

摘要/Abstract

摘要： 为了解藏猪肾脏中促红细胞生成素（erythropoietin，EPO）的表达与其低氧适应之间的关系，采用Western blotting技术检测西藏林芝地区（海拔约3000 m）的高海拔藏猪（ZZ）和高海拔大约克夏猪（YZ）、北京中顺景盛养殖场（海拔约100 m）的低海拔藏猪（ZB）和安徽合肥市（海拔约为36 m）的低海拔大约克夏猪（YH）肾脏组织EPO蛋白的表达量。结果发现，ZZ、ZB、YZ、YH的EPO表达量，在A组试验中，高海拔藏猪与高海拔大约克夏猪差异显著（P<0.05），高海拔大约克夏猪与低海拔藏猪、低海拔大约克夏猪差异极显著（P<0.01）；B组试验中，高海拔藏猪与低海拔藏猪差异不显著（P>0.05），高海拔藏猪与高、低海拔大约克夏猪差异显著（P<0.05）；C组试验中，高、低海拔藏猪间差异显著（P<0.05），低海拔藏猪与高、低海拔大约克夏猪差异极显著（P<0.01）。当大约克夏猪处在高海拔应急状态下时，EPO的表达量高于藏猪。而长期生活在高原的大约克夏猪EPO的表达量较藏猪低，但高于低海拔大约克夏猪。EPO的稳定表达是藏猪适应高原低氧环境的一个重要因素，而高原大约克夏猪肾脏组织内EPO的高表达，则是大约克夏猪适应高原低氧环境的生理调节过程。

关键词: 藏猪; 大约克夏猪; Western blotting; 肾脏组织; EPO蛋白; 差异性; 低氧适应

Abstract: In order to explore the relationship between the expression of erythropoietin (EPO) in kidney tissue of Tibetan pig and hypoxia adaptation, the Western blotting technique was used to detect EPO protein and the kidney tissue which was from Tibetan pig and Large Yorkshire in different altitudes was used in this study. The high altitude Tibetan pigs and Large Yorkshire pigs were from Linzhi of Tibet, and the low altitude were from Beijing and Hefei of Anhui. The results showed that the EPO protein of ZZ，ZB，YZ，YH in A,B and C three groups of experiments was in different expression. Through comparative and analysis, the EPO protein expression was significant difference between high altitude Large Yorkshire pigs and the high altitude Tibetan pigs (P<0.05), and it was extremely significant difference between high altitude Large Yorkshire pigs and the low altitude of Tibetan pigs and Large Yorkshire pigs in group A (P<0.01)；The EPO protein expression was no significant difference between high altitude Tibetan pigs and the low altitude Tibetan pigs (P>0.05), and it was significant difference between high altitude Tibetan pigs and the high and low altitude Large Yorkshire pigs in group B (P<0.05)；The EPO protein expression was significant difference between Tibetan pigs (P<0.05), and it was extremely significant difference between low altitude Tibetan pigs and the high and low altitude Large Yorkshire pigs in group C (P<0.01). When Large Yorkshire pigs were in the high altitude emergency state, the EPO protein expression of Large Yorkshire was higher than Tibetan pigs. When Yorkshire pigs had lived for a long time in the highlands, the EPO protein expression was lower than Tibetan pigs, but higher than the low altitude Yorkshire pigs. The stable expression of EPO was an essential factor of Tibetan pigs to adapt to high altitude hypoxia environment. And the high expression of EPO protein in high altitude Large Yorkshire’s kidney tissue was the physical adjustment process which could help Yorkshire pigs adapt to the plateau low oxygen environment.

Key words: Tibetan pig; Large Yorkshire pig; Western blotting; kidney tissue; EPO protein; difference; hypoxic adaptation

吴洪，德吉巴卓，张健，王亚非，张浩，强巴央宗. 藏猪促红细胞生成素的表达与低氧适应分析[J]. 中国畜牧兽医.

WU Hong,DEJI Bazhuo,ZHANG Jian,WANG Ya-fei,ZHANG Hao,CHAMBA Yangzom. EPO Protein Expression and Hypoxic Adaptation Analysis in Tibetan Pig[J]. .

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