›› 2014, Vol. 41 ›› Issue (12): 98-101.

• 生物技术 • 上一篇    下一篇

新疆也木勒白羊肌肉生长抑制素基因在不同生长时期的表达研究

祖玲玲, 吐来力江·哈木太, 安外尔·热合曼, 决肯·阿尼瓦什   

  1. 新疆农业大学动物科学学院, 新疆乌鲁木齐 830052
  • 收稿日期:2014-05-26 出版日期:2014-12-20 发布日期:2014-12-25
  • 通讯作者: 决肯·阿尼瓦什 E-mail:jueken62@163.com
  • 作者简介:祖玲玲(1988-),女,新疆人,硕士生,研究方向:动物遗传育种.
  • 基金资助:
    国家自然科学基金:MSTN/Smad信号通路与新疆地方绵羊品种肌肉生长发育、产肉性能关联性的研究(31260530).

Expression Levels of MSTN Gene in Different Time of Yemule Sheep

ZU Ling-ling, Tulailijiang·Hamutai, Anwaier·Reheman, Jueken·Aniwashi   

  1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2014-05-26 Online:2014-12-20 Published:2014-12-25

摘要: 试验采用实时荧光定量PCR技术测定也木勒白羊肌肉生长抑制素(myostatin,MSTN)基因在不同生长时期(0~6月龄)的表达量,采用2-ΔΔCt分析不同生长阶段表达量的差异,旨在掌握也木勒白羊不同生长时期MSTN基因的表达规律,为也木勒白羊肌肉生长性状的选育提供遗传学资料.结果发现,MSTN基因在3月龄也木勒白羊肌肉中的表达量显著低于其他月龄(P<0.05),其余各月龄差异不显著(P>0.05);MSTN在脂肪中的表达量0月龄相对较低,随月龄增长,表达量升高,在5月龄达到最高,后随月龄增长表达量降低.MSTN基因在肌肉和脂肪中的表达量随着月龄增长有一定的规律可循,其表达规律可为也木勒白羊肉用性能的选育提供一定的科学依据.

关键词: 也木勒白羊; 肌肉生长抑制素基因; 基因表达

Abstract: Using quantitative PCR investigate myostatin (MSTN) gene expression levels in different time of Yemnle sheep's muscle and fat (0 to 6 month), using 2-ΔΔCt analysis of the different expression levels in different time, and aimed to understand the expression levels of Yemule sheep MSTN gene, provided genetic information for the selection of muscle growth traits. The results showed that MSTN gene expression levels in 3 month was significantly higher than the others months (P<0.05), and the others months had no significant differences in the muscle (P>0.05). In the fat , the expression level of MSTN gene present was lower in 0 month, With the growing increase, the expression levels in MSTN gene has increased, and MSTN gene expression levels was the highest in 5 month, then with the growing increase, the expression levels in MSTN gene had decreased. With the growing increase, MSTN gene expression levels had some certain rules, provided scientific basis for the selection of muscle growth traits.

Key words: Yemule sheep; MSTN gene; gene expression

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