河南省某地区健康人源及动物源肠球菌种属分布及耐药性差异研究

›› 2014, Vol. 41 ›› Issue (12): 172-177.

河南省某地区健康人源及动物源肠球菌种属分布及耐药性差异研究

刘佳¹, 陈霞², 赵爱兰², 袁敏², 熊衍文², 龚林², 卢金星², 李娟², 梁建琴³

1. 河北北方学院, 河北张家口 075000;
2. 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206;
3. 中国人民解放军第309医院结核科, 北京 100091

收稿日期:2014-08-08 出版日期:2014-12-20 发布日期:2014-12-25
通讯作者: 李娟, 梁建琴 E-mail:lijuan@icdc.cn;ljqbj309@163.com
作者简介:刘佳(1988－),女,河北人,硕士,研究方向:内科传染病学.
基金资助:
国家"十二五"艾滋病和病毒性感染等重大传染病防治专项;超耐药菌流行病学和防治技术研究(2013ZX10004-217);传染病预防控制国家重点实验室项目(No.2012SKLID205) .

Study on the Differences of Prevalence and Drug Resistance of Enterococcus Species of Human and Animal Origins in Henan Province, China

LIU Jia¹, CHEN Xia², ZHAO Ai-lan², YUAN Min², XIONG Yan-wen², GONG Lin², LU Jin-xing², LI Juan², LIANG Jian-qin³

1. Hebei North University, Zhangjiakou 075000, China;
2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
3. Department of Tuberculosis, Hospital of the PLA 309, Beijing 100091, China

Received:2014-08-08 Online:2014-12-20 Published:2014-12-25

摘要/Abstract

摘要： 为了解河南省某地区社区健康人源及动物(鸡、猪)源肠球菌种属分布及对多种抗菌药物的耐受情况,以及研究不同来源肠球菌的流行分布和抗菌药物耐药表型特点,本试验应用肠球菌选择性培养基对220份人源及动物源粪便样本进行肠球菌的分离培养,并对分离菌株采用16S rDNA序列分析结合API生化板条进行种属鉴定,纸片扩散法对分离肠球菌进行9种抗菌药物的敏感性检测.肠球菌分离及鉴定结果显示肠球菌总分离率、粪肠球菌分离率及屎肠球菌分离率在人源、鸡源、猪源3种来源间均差异显著(P<0.05):肠球菌总分离率为70.91%(156/220),猪源肠球菌分离率最高(86.00%),人源肠球菌分离率最低(62.63%),且人源与猪源肠球菌分离率差异显著(P<0.018);人源粪便样本中分离率最高的为屎肠球菌(31.36%),鸡源、猪源肠球菌中粪肠球菌分离率最高,分别为28.17%和32.00%.抗菌药物敏感性结果显示肠球菌对多种药物的耐药率在人源、鸡源、猪源3种来源间差异显著(P<0.05),且3种来源肠球菌的多药耐药率差异有统计学意义(P<0.05).人源肠球菌对红霉素(69.35%)、环丙沙星(37.10%)、氨苄西林(19.35%)等抗菌药物耐药率较其他来源的肠球菌要高;鸡源肠球菌对四环素(88.24%)、氟苯尼考(11.76%)、氯霉素(21.57%)等抗菌药物耐药率较其他来源的肠球菌要高;猪源肠球菌对抗菌药物耐药率总体较低,且其多药耐药率(7.84%)也低于人源(35.48%)及鸡源肠球菌(30.19%).提示,健康人及动物粪便样本中肠球菌种属分布不同及对抗菌药物耐药率有差别,并对多种常见抗菌药物耐药率较高,有关部门应加强社区人群及动物等非临床来源肠球菌耐药检查、监测,进而更好的了解中国耐药肠球菌的流行现状,更有效的控制耐药肠球菌的传播.

关键词: 肠球菌; 分离率; 耐药性; 差异性

Abstract: The assay was aimed to evaluate the differences of prevalence and drug resistance of Enterococcus species among human, broilers and swines in Henan province, China. Totally 220 stool samples were collected, and identified by 16S rDNA sequence analysis and the Rapid ID 20 Strep System. There were significant differences among the total isolation rate of Enterococcus, isolation rate of E.faecalis, isolation rate of E.faecium in 3 different origins (P<0.05).A total of 156 (70.91%) Enterococcus spp. isolates were recovered. The isolation rate of Enterococcus from swine origin was the highest (86.00%), while human origin was the lowest (62.63%), There were significant differences between the isolation rates of swine and human origns (P<0.018). The most predominant specie was E.faecium (31.36%) in human, while in broilers and swines were E.faecalis (28.17% and 32.00%, respectively). All isolates were sensitive to vancomycin and teicoplanin. The resistant rate of many multidrug-resistant (MDR) of the isolates from human, broilers and swines were statistically significant (P<0.05).The resistant rates of isolates from human origin Enterococcus spp.to erythromycin (69.35%), ciprofloxacin (37.10%) and ampicilin (19.35%) were higher than those from broiler origin and swine origin. The isolates resistant rates to tetracycline (88.24%), florfenicol (11.76%) and chloramphenicol (21.57%) from broiler origin Enterococcus spp. were higher than those from the other two origins, respectively. The MDR rates of human (35.48%) and broiler (30.19%) origins isolates were higher than swine origin (7.84%). The observation in this study suggested that the prevalence and drug resistance of Enterococcus spp. from human, broilers and swines in Henan province were different, and the MDR isolates were prevalent in various origins Enterococcus. More attention should be given on investigation and research of the resistance of Enterococcus among human and animals to better understand the epidemic of the antidrug-resistant Enterococcus and minimize the spread of it effectively.

Key words: Enterococcus spp.; isolation rate; drug resistance; difference

中图分类号:

S852

刘佳, 陈霞, 赵爱兰, 袁敏, 熊衍文, 龚林, 卢金星, 李娟, 梁建琴. 河南省某地区健康人源及动物源肠球菌种属分布及耐药性差异研究[J]. , 2014, 41(12): 172-177.

LIU Jia, CHEN Xia, ZHAO Ai-lan, YUAN Min, XIONG Yan-wen, GONG Lin, LU Jin-xing, LI Juan, LIANG Jian-qin. Study on the Differences of Prevalence and Drug Resistance of Enterococcus Species of Human and Animal Origins in Henan Province, China[J]. , 2014, 41(12): 172-177.

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