绿汁发酵液、纤维素酶和木聚糖酶对灵芝菌糟发酵饲料品质的影响

›› 2014, Vol. 41 ›› Issue (12): 146-152.

绿汁发酵液、纤维素酶和木聚糖酶对灵芝菌糟发酵饲料品质的影响

陈国富¹, 张文昌¹, 江伟伟¹, 叶杭¹, 朱小清¹, 庄益芬^1,2

1. 福建农林大学动物科学学院, 福建福州 350002;
2. 国家菌草工程技术研究中心, 福建福州 350002

收稿日期:2014-06-11 出版日期:2014-12-20 发布日期:2014-12-25
通讯作者: 庄益芬 E-mail:yfzfz@163.com
作者简介:陈国富(1988-),男,福建人,硕士生,研究方向:动物营养与饲料. 张文昌(1957-),男,内蒙古人,博士,教授,研究方向:动物营养与饲料、动物繁殖生理. 陈国富与张文昌对本文具有同等贡献,并列为第一作者.
基金资助:
福建省教育厅科技项目(JK2009010);国家菌草工程技术研究中心开放基金课题(JCJJ13017).

Effect on the Quality of Fermented Ganodorma Fungus Chaff Feed Supplied by Fermented Green Juice,Cellulase and Xylanase

CHEN Guo-fu¹, ZHANG Wen-chang¹, JIANG Wei-wei¹, YE Hang¹, ZHU Xiao-qing¹, ZHUANG Yi-fen^1,2

1. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. China National Engineering Research Center of Juncao Technology, Fuzhou 350002, China

Received:2014-06-11 Online:2014-12-20 Published:2014-12-25

摘要/Abstract

摘要： 本试验旨在研究绿汁发酵液、纤维素酶和木聚糖酶对灵芝菌糟发酵饲料品质的影响,为利用菌糟生产优质发酵饲料提供理论依据.试验分为7个处理组,以添加蒸馏水为对照(CON组),灵芝菌糟中分别添加2 mL/kg绿汁发酵液(FGJ组)、2000 U/kg纤维素酶(CEL组)、5000 U/kg木聚糖酶(XYL组)、2 mL/kg绿汁发酵液+2000 U/kg纤维素酶(MIX1组)、2 mL/kg绿汁发酵液+5000 U/kg木聚糖酶(MIX2组)、2 mL/kg绿汁发酵液+2000 U/kg纤维素酶+5000 U/kg木聚糖酶(MIX3组),每个处理组3个重复,常温下发酵60 d开封,测定灵芝菌糟发酵饲料的化学成分和发酵品质.结果表明,与CON组相比,FGJ组、CEL组和XYL组可溶性碳水化合物(WSC)含量极显著升高(P<0.01),pH、气体损失率(GLR)及中性洗涤纤维(NDF)、半纤维素(HC)、氨态氮(AN)含量均极显著降低(P<0.01);与FGJ组相比,MIX1组和MIX2组乳酸(LA)、乙酸(AA)含量显著或极显著升高(P<0.05;P<0.01),WSC含量极显著升高(P<0.01),AN含量极显著降低(P<0.01).与CEL组相比,MIX1组的干物质回收率(DMR)及NDF含量极显著降低(P<0.01),WSC、AN含量极显著升高(P<0.01).与MIX1组和MIX2组相比,MIX3组pH、GLR极显著降低(P<0.01),酸性洗涤纤维(ADF)含量显著降低(P<0.05).综上所述,添加纤维素酶和木聚糖酶的效果均优于添加绿汁发酵液,但复合添加效果更佳,其中以MIX3组的添加效果最佳.

关键词: 菌糟; 发酵饲料; 绿汁发酵液; 纤维素酶; 木聚糖酶; 发酵品质

Abstract: This study was aimed to prove up the effect on the quality of fermented ganodorma fungus chaff feed supplied by use the additive, and provided a theoretical basis for the production of high quality of fermented feed.Seven treatments such as 2 mL/kg fermented green juice, 2000 U/kg cellulase, 5000 U/kg xylanase, 2 mL/kg fermented green juice+2000 U/kg cellulase, 2 mL/kg fermented green juice+5000 U/kg xylanase, 2 mL/kg fermented green juice+2000 U/kg cellulase+5000 U/kg xylanase and to add distilled water as control group had been used in this experiment.Each treatment was repeated 3 times.The materials were ensiled at room temperature and opened 60 days later, chemical composition and fermented quality of fungus chaff feed were determined.The results showed that compared with the CON group, the WSC of FGJ, CEL and XYL groups was highly significantly increased (P<0.01), the pH, GLR, NDF, HC and AN of FGJ, CEL and XYL groups were highly significantly reduced (P<0.01).Compared with the FGJ group, the LA and AA of MIX1 and MIX2 groups were significantly or highly significantly reduced (P<0.05;P<0.01), respectively, the WSC of MIX1 and MIX2 groups was highly significantly increased (P<0.01), the AN of MIX1 and MIX2 groups was highly significantly reduced (P<0.01).Compared with the CEL group, the DMR and NDF of MIX1 group were highly significantly reduced (P<0.01), the WSC and AN of MIX1 group were highly significantly increased (P<0.01).Compared with the MIX1 and MIX2 groups, the pH and GLR of MIX3 group were highly significantly reduced (P<0.01), the ADF of MIX3 group was significantly reduced (P<0.05).In conclusion, the effect of adding cellulose and adding xylanase were better than the effect of adding fermented green juice, and the effect of mixed them were better than just one, especially the best of adding effect was MIX3.

Key words: fungus chaff; fermented feed; fermented green juice; cellulase; xylanase; fermentation quality

中图分类号:

S816.5⁺3

陈国富, 张文昌, 江伟伟, 叶杭, 朱小清, 庄益芬. 绿汁发酵液、纤维素酶和木聚糖酶对灵芝菌糟发酵饲料品质的影响[J]. , 2014, 41(12): 146-152.

CHEN Guo-fu, ZHANG Wen-chang, JIANG Wei-wei, YE Hang, ZHU Xiao-qing, ZHUANG Yi-fen. Effect on the Quality of Fermented Ganodorma Fungus Chaff Feed Supplied by Fermented Green Juice,Cellulase and Xylanase[J]. , 2014, 41(12): 146-152.

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