›› 2014, Vol. 41 ›› Issue (11): 283-287.

• 疾病防治 • 上一篇    下一篇

豫西地区市售鸭翅中单增李斯特菌毒力基因的检测

贾艳艳, 何雷, 郁川, 余祖华, 程相朝, 张春杰, 李银聚   

  1. 河南科技大学动物疫病与公共安全重点实验室, 河南省动物疫病与公共安全院士工作站, 河南洛阳 471003
  • 收稿日期:2014-06-10 出版日期:2014-11-20 发布日期:2014-12-06
  • 作者简介:贾艳艳(1983-),女,河南人,博士,讲师,研究方向:分子病原学。
  • 基金资助:
    河南科技大学博士科研启动基金(09001733);河南省教育厅科学技术研究重点项目(14A230005);河南省科技攻关计划项目(142102110039)。

Detection of Virulence Genes of Listeria monocytogenes Isolated from Commercial Duck Wings in the Western Region of Henan Province

JIA Yan-yan, HE Lei, YU Chuan, YU Zu-hua, CHENG Xiang-chao, ZHANG Chun-jie, LI Yin-ju   

  1. Animal Disease and Public Security Academician Workstation of Henan Province, Key Laboratory of Animal Diseases and Public Safety, Henan University of Science and Technology, Luoyang 471003, China
  • Received:2014-06-10 Online:2014-11-20 Published:2014-12-06

摘要: 为分析来自豫西地区市售鸭翅中单增李斯特菌毒力基因的分布变化,本研究以分离自豫西地区市售鸭翅的11株单增李斯特菌分离株为研究对象,利用PCR方法进行8种毒力基因的检测(inlAinlBvirRmprFdltAdltBdltCdltD基因)。结果显示有3株出现了基因缺失,dltA基因检出率为90.9%(10/11),dltC、mprF基因检出率均为81.8%(9/11),其他基因全部呈阳性。这些毒力基因在单增李斯特菌分离株中分布广泛,对豫西地区市售鸭翅具有潜在的威胁,应引起食品监管部门的高度关注。

关键词: 食源性单增李斯特菌; 毒力基因; 分布; 豫西地区; 鸭翅

Abstract: To analyze the virulence genes distribution of Listeria monocytogenes(L.monocytogenes, LM) isolated from commercial duck wings in Luoyang. 8 virulence genes (inlA, inlB, virR, mprF, dltA, dltB, dltC, dltD) from 11 LM isolated strains were detected using PCR method. The results showed that there existed 3 isolated strains with virulence gene-deleted. Detected ratioes for dltA, dltC and mprF were 90.9% (10/11), 81.8% (9/11) and 81.8% (9/11),respectively. The data indicated that these virulence genes were common for LM isolated from commercial duck wings in Luoyang, and some measures were taken to strengthen the surveillance of LM for the potential threat to commercial duck wings safety.

Key words: food-borne Listeria monocytogenes; virulence gene; distribution; the western region of Henan province; duck wings

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