澳洲白绵羊美丽臀和肌肉生长抑制素基因基因型的检测

›› 2014, Vol. 41 ›› Issue (10): 172-178.

澳洲白绵羊美丽臀和肌肉生长抑制素基因基因型的检测

张淑珍, 张莉, 赵福平, 王慧华, 刘瑞凿, 刘真, 樊红樱, 魏彩虹, 杜立新

中国农业科学院北京畜牧兽医研究所, 北京 100193

收稿日期:2014-03-25 出版日期:2014-10-20 发布日期:2014-10-29
通讯作者: 魏彩虹, 杜立新 E-mail:weicaihong@caas.cn;lxdu@263.net
作者简介:张淑珍(1989－),女,陕西人,硕士生,研究方向:动物分子育种。
基金资助:
国家科技支撑计划项目(2011BAD28B05-2)

Detection of CLPG and MSTN Genes Genotypes on AWF Sheep

ZHANG Shu-zhen, ZHANG Li, ZHAO Fu-ping, WANG Hui-hua, LIU Rui-zao, LIU Zhen, FAN Hong-ying, WEI Cai-hong, DU Li-xin

Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2014-03-25 Online:2014-10-20 Published:2014-10-29

摘要/Abstract

摘要： 美丽臀(callipyge,CLPG)及肌肉生长抑制素(myostatin,MSTN)是全世界公认的与肌肉生长发育相关的基因,突变后分别产生“美丽臀”和“双肌”的优良性状。为研究国外进口品种澳洲白绵羊是否携带2种基因的突变位点,本试验以澳洲白绵羊为试验动物,采用PCR-RFLP技术对CLPG、MSTN基因的多态性进行分型。结果发现:CLPG基因上有1个C→T的SNP位点,澳洲白绵羊群体中有CC和CT 2种基因型;MSTN基因在3'-UTR区发现1个G→A的SNP位点,澳洲白绵羊群体中有GG和GA 2种基因型。用R语言程序对所测数据进行性别间不同基因型与生长发育状况的单因素方差分析得知,CLPG和MSTN基因不同基因型对澳洲白公羊体重、体高、体斜长、尻宽、背膘厚和眼肌面积均无显著性影响(P>0.05);CLPG基因CT基因型在母羊体重上显著高于CC基因型(P<0.05),在体斜长上极显著高于CC基因型(P<0.01),MSTN基因GA基因型母羊眼肌面积极显著高于GG基因型(P<0.01)。澳大利亚进口澳洲白绵羊中筛选到含有CLPG和MSTN基因的突变位点,且该突变位点对绵羊体尺性状有显著性作用,因此澳洲白绵羊引进后可以作为改良本地羊品种、基因聚合及三元配套系的终端父本。

关键词: 澳洲白绵羊; 美丽臀; 肌肉生长抑制素; PCR-RFLP

Abstract: Callipyge (CLPG) and myostatin (MSTN) are recognized as the growth of muscle-related genes in the world,which produce "beautiful buttocks " and "double-muscle" good traits after mutation. To study whether imported AWF sheep carrying mutations in two genes,we used PCR-RFLP technique to check. The results showed that there was a C→T SNP in CLPG gene,AWF sheep populations had CC and CT genotypes;A G→A SNP locus in the 3'-UTR region of MSTN gene, AWF sheep populations had GG and GA genotypes. Analysis of variance with the R language between different genotypes and growth in different gender,MSTN and CLPG of different genotypes on the AWF rams weight,height,length,rump width,fat thickness and loin area were no significant differences (P>0.05);CLPG CT genotype on ewe weight was significantly higher than CC genotype (P<0.05),in length was very significantly higher than CC genotype (P<0.01),MSTN GA genotype on ewe loin area was very significantly higher than GG genotype (P<0.01). We detected contain CLPG and MSTN mutations in imported AWF sheep from Australia,and the mutation of sheep body traits had significant effects.Therefore,the AWF sheep could used as improved local sheep breeds,gene pyramiding and terminals supporting male in ternary system after the introduction.

Key words: AWF sheep; CLPG; MSTN; PCR-RFLP

中图分类号:

S813.3

张淑珍, 张莉, 赵福平, 王慧华, 刘瑞凿, 刘真, 樊红樱, 魏彩虹, 杜立新. 澳洲白绵羊美丽臀和肌肉生长抑制素基因基因型的检测[J]. , 2014, 41(10): 172-178.

ZHANG Shu-zhen, ZHANG Li, ZHAO Fu-ping, WANG Hui-hua, LIU Rui-zao, LIU Zhen, FAN Hong-ying, WEI Cai-hong, DU Li-xin. Detection of CLPG and MSTN Genes Genotypes on AWF Sheep[J]. , 2014, 41(10): 172-178.

参考文献

1 李云龙,杨章平,毛永江,等.DLK1 基因的单核苷酸多态性与绵羊生长发育性状的相关性分析[J]. 中国畜牧杂志,2011,47(1):18~20.
2 刘铮涛,李祥龙,冯敏山,等. MSTN基因内含子2多态性与山羊体重性状相关研究[J].畜牧兽医学报,2006,37(8):745~748.
3 孟详人,马月辉,关伟军,等. 4个绵羊品种双肌臀基因基因型的检测[J]. 中国畜牧兽医,2009,36(4):119~121.
4 Clop A,Marcq F,Takeda H,et al. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep[J]. Nat Genet,2006,38(7):813~818.
5 Cockett N E,Jackson S P,Shay T L,et al. Chromosomal localizationof the callipyge gene in sheep (Ovis aries) using bovine DNA markers[J]. Proc Natl Acad Sci USA,1994,91(8):3019~3023.
6 Cockett N E,Jackson S P,Shay T L,et al. Polar overdominance at the ovine callipyge locus[J]. Science,1996,273(5272):236~238.
7 Dunner S,Miranda M E,Amigues Y,et al. Haplotype diversity of the myostatin gene among beef cattle breeds[J].Genet Sel Evol,2003,35(1):103~108.
8 Freking B A,Murphy S K,Wylie A A,et al. Identification of the single base change causing the callipyge muscle hypertrophy phenotype,the only known example of polar overdominance in mammals[J]. Genome Res,2002,12(10):1496~1506.
9 Gill J L,Bishop S C,McCorquodale C,et al. Associations between the 11-bp deletion in the myostatin gene and carcass quality in angus-sired cattle[J].Animal Genetics,2009,40(1):97~100.
10 Jackson S P,Miller M F,Green R D. Phenotypic characterization of rambouillet sheep expression the callipyge gene:Ⅲ. Muscle weights and muscle weight distribution[J]. Anim Sci,1997,75:133~138.
11 Lee S J,Mcpherron A C. Myostatin and the control of skeletal muscle mass[J]. Curr Opin Genet Dev,1999,9(5):604~607.
12 Leymaster K A,Freking B A. Genetic variations and associations for improving meat production and meat qualities in sheep and goats[A]. The 6th World Congr Genet Appl Livest Prod[C].1998.
13 Mcpherron A C,Lawler A M,Lee S J. Regulation of skeletal muscle mass in mice by a new TGF-β superfamily member[J]. Nature,1997,387(6628):83~90.
14 Meyer H H,Haribaskar S,Abdulkhaliq A M,et al. Callipyge gene effects on lamb growth,carcass traits,muscle weights and meat characteristics[A]. The 6th World Congr Genet Appl Livest Prod[C].1998.
15 Wiener P,Woolliams J A,Frank-Lawale A,et al. The effect of a mutation in the myostatin gene on meat and carcass quality[J]. Meat Science,2009,83(1):127~134.

[1]	高倍瑶, 刘艳光, 贾琪, 柳俭强, 罗新惠, 张立春. 不同品种猪PBD-124基因多态性及差异表达研究[J]. 中国畜牧兽医, 2022, 49(6): 2208-2215.
[2]	盛辉, 郭益文, 张林林, 谭皓云, 胡德宝, 李新, 丁向彬, 郭宏. MSTN基因对牛肌动蛋白细胞骨架调节通路的影响[J]. 中国畜牧兽医, 2021, 48(10): 3554-3564.
[3]	盛辉, 张林林, 郭益文, 李新, 丁向彬, 郭宏. 干扰MSTN的牛骨骼肌卫星细胞实时荧光定量PCR内参基因的筛选[J]. 中国畜牧兽医, 2020, 47(2): 381-391.
[4]	朱菲菲, 张俊星, 张林林, 李新, 刘新峰, 郭益文, 丁向彬. 干扰MSTN对牛骨骼肌卫星细胞增殖分化的影响[J]. 中国畜牧兽医, 2020, 47(2): 479-487.
[5]	薛霖莉, 李艺雷, 候亚琴, 冀云燕, 任华伟, 耿建军, 王海东, 赫晓燕, 邢海云. 肌肉生长抑制素在小鼠不同发育阶段心肌组织中的表达[J]. 《中国畜牧兽医》, 2018, 45(6): 1479-1485.
[6]	吴鹏飞, 王冬, 张向前, 张丽, 丁馥香, 张跟喜. 边鸡IGF-ⅠR基因AluⅠ位点多态性及其对生长性状和繁殖性状的遗传效应分析[J]. 《中国畜牧兽医》, 2017, 44(3): 813-818.
[7]	宋兴超, 徐超, 刘宗岳, 杨童奥, 岳志刚, 刘琳玲, 丛波, 杨福合. 水貂TYR基因T138A位点多态性及其与毛色性状的关联分析[J]. 《中国畜牧兽医》, 2017, 44(10): 2973-2979.
[8]	刘海霞, 朱爱文. 猪IGFBP2基因遗传多态性及其与肉质性状的关联分析[J]. 《中国畜牧兽医》, 2017, 44(10): 3016-3022.
[9]	李聪, 曹文广. CRISPR/Cas9介导的绵羊胎儿成纤维细胞肌肉生长抑制素基因敲除研究[J]. , 2015, 42(11): 2813-2821.
[10]	周艳, 韩海霞, 逯岩, 曹顶国, 李福伟, 刘玮, 高金波, 雷秋霞. 鸡脂滴包被蛋白基因内含子6多态性与胴体及脂肪性状的相关性研究[J]. , 2015, 42(10): 2695-2700.
[11]	朱和平，吴凯峰，苏小虎，王一超，周欢敏，赵瑞媛，张焱如. 牛肌肉生长抑制素基因打靶载体的构建与鉴定[J]. 中国畜牧兽医, 2014, 41(6): 34-37.
[12]	易恒洁，李辉，杨胜林，赵忠海，彭邦星. 三穗鸭肌肉生长抑制素基因的生物信息学分析[J]. 中国畜牧兽医, 2014, 41(4): 1-6.
[13]	祖玲玲, 吐来力江·哈木太, 安外尔·热合曼, 决肯·阿尼瓦什. 新疆也木勒白羊肌肉生长抑制素基因在不同生长时期的表达研究[J]. , 2014, 41(12): 98-101.
[14]	彭邦星, 李辉, 易恒洁, 赵忠海. 兴义鸭肌肉生长抑制素基因多态性与屠宰性状的相关性分析[J]. , 2014, 41(12): 225-230.
[15]	李盈, 额尔敦木图, 刘图雅, 伊特格勒图, 沙日扣, 图雅. 肌肉生长抑制素基因在不同动物中的研究进展[J]. 中国畜牧兽医, 2013, 40(8): 141-145.