高效液相色谱法测定生乳中双乙酸钠

›› 2013, Vol. 40 ›› Issue (S1): 37-38.

高效液相色谱法测定生乳中双乙酸钠

张养东^1,2,3, 郑楠^1,2,3, 李松励^1,2,3, 郑姗姗^1,2,3, 许晓敏^1,2, 王加启^1,2,3

1. 中国农业科学院北京畜牧兽医研究所, 农业部奶产品质量安全风险评估实验室(北京), 北京 100193;
2. 农业部奶及奶制品质量监督检验测试中心(北京), 北京 100193;
3. 动物营养学国家重点实验室, 北京 100193

收稿日期:2013-08-20 出版日期:2013-09-30 发布日期:2013-11-29
通讯作者: 郑楠 E-mail:nanzheng.cn@gmail.com
作者简介:张养东（1982-），男，山东人，博士，研究方向：奶牛营养与牛奶质量安全。
基金资助:
现代农业产业技术体系专项资金（nycytx-04-01）和生鲜乳质量安全风险评估专项资助。

Determination of Sodium Hydrogen Diacetate in Raw Milk using High Performance Liquid Chromatography

ZHANG Yang-dong^1,2,3, ZHENG Nan^1,2,3, LI Song-li^1,2,3, ZHENG Shan-shan^1,2,3, XU Xiao-min^1,2, WANG Jia-qi^1,2,3

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Ministry of Agriculture-Milk Risk Assessment Laboratory, Beijing 100193, China;
2. Ministry of Agriculture-Milk and Dairy Product Inspection Center, Beijing 100193, China;
3. State Key Laboratory of Animal Nutrition, Beijing 100193, China

Received:2013-08-20 Online:2013-09-30 Published:2013-11-29

摘要/Abstract

摘要： 试验旨在建立高效液相色谱（HPLC）测定生乳中双乙酸钠的方法。生乳样品经磷酸变性沉淀蛋白，并将样品中双乙酸钠酸化转化为乙酸，定容，离心取上清液，过微孔滤膜后，以磷酸二氢铵为流动相进行HPLC检测，外标法定量。在0.05~0.50 mg/mL浓度范围内，标准曲线R²=0.997，样品加标回收率在90%~110%之间，相对标准偏差1.0%~4.1%。此方法检出限为0.05 g/kg。

关键词: 高效液相色谱色谱(HPLC); 生乳; 双乙酸钠

Abstract: A high performance liquid chromatography (HPLC) method was developed for determination of sodium hydrogen diacetate in raw milk. Protein was denatured with phosphoric acid then precipitated, and transfer sodium hydrogen diacetate into acetic acid in raw milk, constant volume, centrifuged and the supernatant sample over the membrane. The target compound was analyzed by HPLC with ammonium dihydrogen phosphate as mobile phase, the target was identified and determined by external standard method. In the concentration range of 0.05 to 0.50 mg/mL, R² of calibration curve for the object was above 0.99. The recovery of target compound ranged from 90% to 110% with relative standard deviations of 1.0% to 4.1%. The detected limit was 0.05 g/kg,

Key words: HPLC; raw milk; sodium hydrogen diacetate

中图分类号:

S859.84

张养东, 郑楠, 李松励, 郑姗姗, 许晓敏, 王加启. 高效液相色谱法测定生乳中双乙酸钠[J]. , 2013, 40(S1): 37-38.

ZHANG Yang-dong, ZHENG Nan, LI Song-li, ZHENG Shan-shan, XU Xiao-min, WANG Jia-qi. Determination of Sodium Hydrogen Diacetate in Raw Milk using High Performance Liquid Chromatography[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(S1): 37-38.

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