›› 2013, Vol. 40 ›› Issue (S1): 39-41.

• 安全检测 • 上一篇    下一篇

酶联免疫法对生鲜乳中黄曲霉毒素M1的快速检测

蒙君丽1, 宋荪阳1, 郑百芹1, 齐彪2, 张建民1, 葛怀礼1, 吴颖3, 李爱军1   

  1. 1. 唐山市畜牧水产品质量监测中心, 河北唐山 063000;
    2. 中央农业广播电视学校唐山分校, 河北唐山 063000;
    3. 唐山科技情报研究所, 河北唐山 063000
  • 收稿日期:2013-08-02 出版日期:2013-09-30 发布日期:2013-11-29
  • 通讯作者: 宋荪阳
  • 作者简介:蒙君丽(1973-),女,陕西人,学士,水产高级工程师,从事畜产品检验工作。
  • 基金资助:
    生鲜牛乳质量安全监测及合理日粮保障技术研究与示范(11221001D)。

ELISA for Rapid Detection of Aflatoxin M1 in Raw Milk

MENG Jun-li1, SONG Sun-yang1, ZHENG Bai-qin1, QI Biao2, ZHANG Jian-min1, GE Huai-li1, WU Ying3, LI Ai-jun1   

  1. 1. Tangshan Livestock and Aquatic Products Quality Monitoring Center, Tangshan 063000, China;
    2. The Central Agricultural Broadcasting and Television School of Tangshan Branch, Tangshan 063000, China;
    3. Tangshan Science and Technology Information Institute Tangshan 063000, China
  • Received:2013-08-02 Online:2013-09-30 Published:2013-11-29

摘要: 采用酶联免疫法(ELISA)对生鲜乳中黄曲霉毒素M1进行检测,对比了3个不同厂家的试剂盒检测结果。结果表明,在空白样品中添加浓度为0.5、1.0、2.5 μg/kg时,试剂盒的回收率在93.5%~103.5%。不同厂家的试剂盒测定结果重复性、精确度均较好,说明酶联免疫法能够有效检测生鲜乳中黄曲霉毒素M1

关键词: 生鲜乳; 黄曲霉毒素M1; 酶联免疫法

Abstract: ELISA for the analysis of aflatoxin M1 in raw milk were determined.The experiment compared the results of enzyme-linked immunosorbent assay kits from different manufacturers. The results showed that when the samples were spiked with 0.5,1.0 and 2.5 μg/kg of aflatoxin M1, the recoveries were 93.5% to 103.5% by ELISA. ESLISA is an effective method for detecting of aflatoxin M1 in raw milk.

Key words: raw milk; aflatoxin M1; ELISA

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