常规高效液相色谱仪—壳核填料色谱柱快速测定黄曲霉毒素M1

摘要/Abstract

摘要： 作者使用常规高效液相色谱仪大体积进样技术2.6 μm壳核填料色谱柱快速测定黄曲霉毒素M₁。结果表明，在常规液相色谱仪上使用壳核填料色谱柱比传统全多孔填料色谱柱更加快速有效。与常规全多孔3 μm （4.6 mm×150 mm）和5 μm（4.6 mm×250 mm）填料色谱柱相比，相同仪器条件下使用洗脱强度低于流动相的溶剂定容上机，2.6 μm（4.6 mm×100 mm）核壳结构色谱柱的分析时间分别为后两者的51.5%和21.3%，峰高分别为后两者的183.9%和490.5%，峰方差分别为后两者的34.7%和6.2%。因此，常规HPLC上使用壳核填料色谱柱可以在大幅减少分析时间和成本的同时增加检测的灵敏度和准确性。

关键词: 高效液相色谱; 核壳填料色谱柱; 大体积进样; 黄曲霉毒素M₁

Abstract: Herein, aflatoxin M₁(AFM₁) was analysed on a conventional HPLC by a chromatography column packed with 2.6 μm core-shell particles and large volumn injection technique. The results show that the core-shell column is faster and more effective than typical total porous particles packed column even on conventional HPLC. With the same instrumental condition and weak injection solvent, the analysis time of new 2.6 μm core-shell particle column (4.6 mm×100 mm) is 51.5% and 21.3% of tranditional total porous 3 μm particle(4.6 mm×150 mm) and 5μm particle(4.6 mm×250 mm) columns respectively. Meanwhile, the peak height is 183.9% and 490.5% of the latter columns and the peak variances is 34.7% and 6.2% of the latter columns. This indicates that the use of core-shell columns on conventional HPLC leads to the reductions of analysis time and cost with increased sensitivity and accuracy.

Key words: HPLC; core-shell column; large volumn injection; aflatoxin M₁

中图分类号:

TS252.7

雷绍荣, 毛建霏, 付成平, 胡莉, 仲伶俐. 常规高效液相色谱仪—壳核填料色谱柱快速测定黄曲霉毒素M₁[J]. , 2013, 40(S1): 42-46.

LEI Shao-rong, MAO Jian-fei, FU Cheng-ping, HU Li, ZHONG Ling-li. Fast Quantification of Aflatoxin M₁ with a Conventional HPLC-FLD using a Column Packed with Core-shell Particles[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(S1): 42-46.

参考文献

1 ALEXANDER A J, WAEGHE T J, HIMES K W, et al. 2011. Modifying conventional high-performance liquid chromatography systems to achieve fast separations with Fused-Core columns: A case study. Journal of Chromatography A[J], 1218(32): 5456~5469.
2 BAKALYAR S R, PHIPPS C, SPRUCE B, et al. 1997. Choosing sample volume to achieve maximum detection sensitivity and resolution with high-performance liquid chromatography columns of 1.0, 2.1 and 4.6 mm I.D. Journal of Chromatography A[J], 762(1-2): 167~185.
3 IARC 2002. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans,. 82, Some Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. Lyon, France: World Health Organization (WHO), IARC Press.
4 CARR P W, STOLL D R, WANG X 2011. Perspectives on Recent Advances in the Speed of High-Performance Liquid Chromatography. Analytical Chemistry[J], 83(6): 1890~1900.
5 FEKETE S, OL H E, FEKETE J 2012. Fast liquid chromatography: The domination of core-shell and very fine particles. Journal of Chromatography A[J], 1228(0): 57~71.
6 GEDICKE K, ANTOS D, SEIDEL-MORGENSTERN A 2007. Effect on separation of injecting samples in a solvent different from the mobile phase. Journal of Chromatography A[J], 1162(1): 62~73.
7 GRITTI F, FELINGER A, GUIOCHON G 2006. Influence of the errors made in the measurement of the extra-column volume on the accuracies of estimates of the column efficiency and the mass transfer kinetics parameters. Journal of Chromatography A[J], 1136(0): 57~72.
8 GRITTI F, GUIOCHON G 2008. The ultimate band compression factor in gradient elution chromatography. Journal of Chromatography A[J], 1178(1-2): 79~91.
9 GRITTI F, GUIOCHON G 2010. Performance of columns packed with the new shell Kinetex-C18 particles in gradient elution chromatography. Journal of Chromatography A[J], 1217(10): 1604~1615.
10 GRITTI F, GUIOCHON G 2011. Theoretical investigation of diffusion along columns packed with fully and superficially porous particles. Journal of Chromatography A[J], 1218(22): 3476~3488.
11 GRITTI F, GUIOCHON G 2012. The current revolution in column technology: How it began, where is it going? Journal of Chromatography A[J], 1228(9): 2~19.
12 GRITTI F, SANCHEZ C A, FARKAS T, et al. 2010. Achieving the full performance of highly efficient columns by optimizing conventional benchmark high-performance liquid chromatography instruments. Journal of Chromatography A[J], 1217(18): 3000~3012.
13 GUIOCHON G, GRITTI F 2011. Shell particles, trials, tribulations and triumphs. Journal of Chromatography A[J], 1218(15): 1915~1938.
14 HAN R W, ZHENG N, WANG J Q, et al. 2013. Survey of aflatoxin in dairy cow feed and raw milk in China. Food Control[J], 34(1): 35~39.
15 HORV TH K, GRITTI F, FAIRCHILD J N, et al. 2010. On the optimization of the shell thickness of superficially porous particles. Journal of Chromatography A[J], 1217(41): 6373~6381.
16 MAO J, LEI S, YANG X, et al. 2013. Quantification of ochratoxin A in red wines by conventional HPLC-FLD using a column packed with core-shell particles. Food Control[J], 32(2): 505~511.
17 Nunez, GALLART-AYALA H, MARTINS C P B, et al. 2012. New trends in fast liquid chromatography for food and environmental analysis. Journal of Chromatography A[J], 1228(9): 298~323.
18 FAO 2004. Worldwide Regulations for Mycotoxins in Food and Feed in 2003. Food and Nutrition Paper, 81. Rome: FAO.[J].
19 SANCHEZ A C, ANSPACH J A, FARKAS T 2012. Performance optimizing injection sequence for minimizing injection band broadening contributions in high efficiency liquid chromatographic separations. Journal of Chromatography A[J], 1228(9): 338~348.
20 VANMIDDLESWORTH B J, DORSEY J G 2012. Quantifying injection solvent effects in reversed-phase liquid chromatography. Journal of Chromatography A[J], 1236(0): 77~89.

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常规高效液相色谱仪—壳核填料色谱柱快速测定黄曲霉毒素M₁

Fast Quantification of Aflatoxin M₁ with a Conventional HPLC-FLD using a Column Packed with Core-shell Particles

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