›› 2013, Vol. 40 ›› Issue (9): 222-224.

• 经验交流 • 上一篇    下一篇

高效液相色谱法测定金荞麦超微粉原花青素B2含量

王航, 汤承, 岳华   

  1. 西南民族大学生命科学与技术学院, 四川成都 610041
  • 修回日期:2013-06-05 出版日期:2013-09-20 发布日期:2013-09-18
  • 作者简介:王航(1989-),男,河南人,硕士生,研究方向:动物传染病的防治。
  • 基金资助:
    "十二五"国家科技支撑计划(2011BAD34B02-4)。

Determination of Proantho Cyanidins B2 of Fagopyrum dibotryis Superfine Powder by HPLC

WANG Hang, TANG Cheng, YUE Hua   

  1. School of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Revised:2013-06-05 Online:2013-09-20 Published:2013-09-18

摘要: 试验为测定金荞麦超微粉中有效成分原花青素B2的含量,将四川金荞麦饮片粉碎至0.75 nm以下的超微粉,50%乙醇提取,并利用高效液相色谱测定原花青素B2的含量。结果显示,在0.0352~1.4080 μg/mL范围内原花青素B2与峰面积线性关系良好,回归方程为Y=59797X+592.3,r=0.996,金荞麦超微粉中原花青素B2含量在1.305~1.541 mg/g之间。本试验结果表明该方法能快速、稳定、精确的检测原花青素B2含量,为金荞麦制剂质量控制提供了科学依据。

关键词: 金荞麦; 超微粉; 高效液相色谱法; 原花青素B2

Abstract: To determine the content of proantho cyanidins B2 in Fagopyrum dibotryis superfine powder, the Fagopyrum dibotryis crushed less than 0.75 nm superfine powder extracted by 50% ethanol,an HPLC method was used. Epicatechin showed a good linear relationship in 0.0352 to 1.4080 μg/mL and the regression equation was Y=59797X+592.3,r=0.996. Proantho cyanidins B2 of Fagopyrum dibotryis superfine powder content was between 1.305 to 1.541 mg/g.The method was simple and suitable for determing the content of proantho cyanidins B2 in Fagopyrum dibotryis superfine powder. It provided the scientific basis for Fagopyrum dibotryis preparation quality control.

Key words: Fagopyrum dibotryis; superfine powder; HPLC; proantho cyanidins B2

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