N-乙酰-L-半胱氨酸对Ⅰa型牛源无乳链球菌诱导的小鼠肝脏氧化损伤的保护作用

中国畜牧兽医 ›› 2013, Vol. 40 ›› Issue (8): 78-82.

N-乙酰-L-半胱氨酸对Ⅰa型牛源无乳链球菌诱导的小鼠肝脏氧化损伤的保护作用

杨峰, 王旭荣, 李新圃, 罗金印, 王玲, 李宏胜

中国农业科学院兰州畜牧与兽药研究所, 甘肃省中兽药工程技术研究中心, 农业部兽用药物创制重点实验室, 甘肃兰州 730050

收稿日期:2013-04-27 出版日期:2013-08-20 发布日期:2013-08-16
通讯作者: 李宏胜 E-mail:lihsheng@sina.com
作者简介:杨峰(1985- ),男,陕西人,硕士,实习研究员,研究方向:预防兽医学。
基金资助:
中央级公益性科研院所基本科研业务费专项资金项目(1610322013002);"十二五"国家科技支撑项目(2012BAD12B03);甘肃省农业生物技术项目(GNSW-2008-03)。

Beneficial Effect of N-acetylcysteine against Oxidative Damage in Mice Liver Induced by Streptococcus agalactiae Serotype Ⅰa Isolated from Cow

YANG Feng, WANG Xu-rong, LI Xin-pu, LUO Jin-yin, WANG Ling, LI Hong-sheng

Key Laboratory of Veterinary Pharmaceutics Discovery, Ministry of Agriculture, Engineering and Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou 730050, China

Received:2013-04-27 Online:2013-08-20 Published:2013-08-16

摘要/Abstract

摘要： 试验旨在研究Ⅰa型牛源无乳链球菌感染小鼠后诱导的肝脏组织氧化损伤及N-乙酰-L-半胱氨酸(NAC)在氧化损伤过程中所起的作用。40只小鼠随机分为对照组、NAC处理组、无乳链球菌感染组和NAC+无乳链球菌处理组,试验期为10 d。采用比色法测定各组试验小鼠肝脏组织中的谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽S-转移酶(GST)和过氧化物酶(POD)的活性及丙二醛(MDA)含量,并进行统计分析。结果显示,与对照组相比,无乳链球菌感染组肝脏GSH-Px、GST和POD活性极显著降低(P<0.01),MDA含量极显著升高(P<0.01);NAC处理组肝脏GSH-Px、GST和POD活性极显著升高(P<0.01),MDA含量极显著降低(P<0.01)。NAC+无乳链球菌处理组与无乳链球菌感染组相比,GSH-Px、GST和POD活性极显著升高(P<0.01),MDA含量极显著降低(P<0.01);而与对照组相比,GSH-Px、GST和POD活性极显著降低(P<0.01),MDA含量极显著增高(P<0.01)。结果表明,Ⅰa型牛源无乳链球菌感染,能诱导小鼠肝脏组织的氧化损伤;100 mg/kg体重的NAC预处理能显著增强Ⅰa型牛源无乳链球菌感染小鼠肝脏中GSH-Px、GST和POD的活性,显著减小MDA含量,可减轻该菌诱导的氧化损伤,但不足以使感染小鼠的这些指标恢复至正常水平。

关键词: N-乙酰-L-半胱氨酸; Ⅰa型; 无乳链球菌; 氧化损伤

Abstract: The assay was aimed to investigate the effect of Streptococcus agalactiae on oxidative damage in mice liver and the beneficial effect of N-acetylcysteine against the damage. Forty adult mice were divided into 4 groups (control, NAC, S.agalactiae and NAC+S.agalactiae) randomly. Colorimetry was used to detect the content of MDA and the activities of GSH-Px, GST and POD. The results showed that, compared with the control group, there were extremly significant reductions in the activity levels of GSH-Px, GST and POD (P<0.01) accompanied by a extremly significant increase in the MDA content (P<0.01) in bacteria infected group; extremly significant increases in the activity levels of GSH-Px, GST and POD (P<0.01) accompanied by a extremly significant reduction in the MDA content (P<0.01) in NAC group.Compared with S.agalactiae group, extremly significant increases in the activities of GSH-Px, GST and POD (P<0.01), and extremly significant reduction of the MDA content (P<0.01) could be observed in NAC+S.agalactiae group, but when it came to control group, extremly significant reduction in the activities of GSH-Px, GST and POD (P<0.01), and extremly significant increase of the MDA content (P<0.01) could be observed. Thus, the results suggested that infection of Ⅰa S.agalactiae isolated from bovine could induce oxidative damage in mice liver, and NAC (100 mg/(kg·BW)) pretreatment could enhance the activities of GSH-Px, GST and POD, and decrease the MDA content to reduce the damage degree.

Key words: N-acetylcysteine; serotype Ⅰa; Streptococcus agalactiae; oxidative damage

中图分类号:

杨峰, 王旭荣, 李新圃, 罗金印, 王玲, 李宏胜. N-乙酰-L-半胱氨酸对Ⅰa型牛源无乳链球菌诱导的小鼠肝脏氧化损伤的保护作用[J]. 中国畜牧兽医, 2013, 40(8): 78-82.

YANG Feng, WANG Xu-rong, LI Xin-pu, LUO Jin-yin, WANG Ling, LI Hong-sheng. Beneficial Effect of N-acetylcysteine against Oxidative Damage in Mice Liver Induced by Streptococcus agalactiae Serotype Ⅰa Isolated from Cow[J]. , 2013, 40(8): 78-82.

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