抗鸡大肠杆菌O78单味中药及有效成分的筛选

摘要/Abstract

摘要： 在中兽医药学理论指导下,根据相关文献报道确定了清热类、祛湿类、理血类、理气类等114味中草药,以鸡大肠杆菌O78作为目标菌株,进行体外抑菌试验,筛选出6种具有一定抗菌作用的中药,包括芦荟、穿心莲、大蒜、锦灯笼、乌梅和石榴皮,其抑菌圈分别是15、12、12、11、11和7 mm。运用硅胶柱层析、凝胶柱层析、ODS柱层析对芦荟的抗菌成分进行了分离、纯化,经质谱、红外光谱和核磁共振分析得到抗菌单体物质,确定为延胡索酸。进一步抗菌活性研究结果显示,延胡索酸对金黄色葡萄球、链球菌、大肠杆菌和沙门氏菌的最小抑制浓度(MIC)分别是50、50、780和780 μg/mL,最小杀菌浓度(MBC)分别是100、100、1560和780 μg/mL。

关键词: 鸡大肠杆菌; 筛选; 中药; 芦荟

Abstract: According to pertinent literature, we chose 114 kinds of Chinese material medcine including heat-clearing drugs, dampness-eliminating drugs, blood-regulating drugs and others to examine their antibacterial activity against APEC through bacteriostasis test in vitro. 6 kinds of Chinese material medcine had good antibacterial activity, such as aloe, andrographitis, garlic, calyx seu fructus physalis, fructus mume and granati cortex. Their inhibition zones were respectively 15, 12, 12, 11, 11 and 7 mm. Then, we isolated and purified the antibacterial components of aloe by silica gel column chromatography, gel column chromatography and ODS column chromatography. At last, the antibacterial component was identified as fumaric acid through MS, IR and NMR analysis. We further studied the activity of fumaric acid against Escherichia coli, Staphylococcus aureus, Streptococcus and Salmonella. The MIC was respectively 50, 50, 780 and 780 μg/mL and MBC was respectively 100, 100, 1560 and 780 μg/mL.

Key words: pathogenic avian Escherichia coli; screen; Chinese herbal medicines; aloe

中图分类号:

S858.31

贺常亮, 韦旭斌, 付本懂, 蒋小林, 吕程, 张伟. 抗鸡大肠杆菌O78单味中药及有效成分的筛选[J]. , 2013, 40(6): 178-184.

HE Chang-liang, WEI Xu-bin, FU Ben-dong, JIANG Xiao-lin, LÜ Cheng, ZHANG Wei. Screening of Chinese Herbal Medicines and Active Components for against Pathogenic Avian Escherichia coli O78[J]. , 2013, 40(6): 178-184.

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