猪繁殖与呼吸综合征的研究进展

摘要/Abstract

摘要： 猪繁殖与呼吸综合征是目前危害养猪业的一种严重的病毒性疾病,给养猪业造成了巨大的经济损失。文章对最近国内外猪繁殖与呼吸综合征病毒分子生物学及分子免疫学两方面的成果进行了概述,旨在为猪繁殖与呼吸综合征的防制提供新的思路和方法。

Abstract: Porcine reproductive and respiratory syndrome (PRRS) is presently one of the most important viral infectious disease of pigs. This article reviews the latest research progress on PRRS in recent years, particularly in the immunology and molecular biology. It will provide guidances and directions for the prevention and treatment of PRRS.

Key words: PRRSV; molecular biology; immunology 

中图分类号:

S852.65⁺9.6

郭敏, 郎广平, 冯娇, 李彬, 付云云, 单悦, 赵建增, 高英杰. 猪繁殖与呼吸综合征的研究进展[J]. , 2013, 40(4): 211-215.

GUO Min, LANG Guang-ping, FENG Jiao, LI Bin, FU Yun-yun, SHAN Yue, ZHAO Jian-zeng, GAO Ying-jie. Research Progress of Porcine Reproductive and Respiratory Syndrome[J]. , 2013, 40(4): 211-215.

参考文献

1 王剑非,孙建全,张金强,等.PRRSV分离株Nsp2基因和ORF5基因的遗传变异分析[J].家畜生态学报,2011,32(1):48~53.
2 白雪,付云云,郭敏,等.CSF免疫状态下猪PRRS易感性及INF-γ分泌细胞的应答[J].中国兽医学报,2012,32(1):5~7.
3 朱紫祥,吴发兴,王晶钰,等.我国华东某省HP-PRRSV的分离鉴定及全基因组序列分析[J].西北农林科技大学学报(自然版),2010,38(8):25~32.
4 吴锦艳,田宏,尚佑军,等.高致病性猪蓝耳病病毒GS/LZh/07株的分离、鉴定及其非结构蛋白Nsp2基因特性分析[J].畜牧兽医学报,2009,40(3):438~443.
5 张汉云,赵彦宗,贺东生.猪高致病性蓝耳病免疫研究进展.金宁一,高宏伟,罗廷荣.中国畜牧兽医学会家畜传染病学分会第七届全国会员代表大会暨第十三次学术研讨会论文集.南宁:中国畜牧兽医学会,2009.
6 张得玉,康艳梅,张桂红,等.猪生殖与呼吸综合征病毒RT-LAMP检测方法的建立[J].中国兽医科学,2009,39(10):890~893.
7 张燕霞,吴发兴,刘爽,等.猪蓝耳病病毒通用RT-PCR检测方法的建立及应用[J].中国兽药杂志,2011,45(12):13~15,34.
8 李贺,刘茂军,甘源,等.4株高致病性猪繁殖与呼吸障碍综合征病毒的分离与鉴定[J].江苏农业学报,2008,24(4):444~450.
9 杨利琴,庞海洋,石凤海,等.云南高致病性PRRSV Nsp2和ORF5基因变异分析[J].动物医学进展,2010,31(11):15~22.
10 单悦,高英杰,赵建增,等. 猪繁殖与呼吸综合征病毒分子生物学研究[J].中国畜牧兽医, 2012, 39(4): 47~49.
11 屈雪琪,赵建增,梁智选,等.猪繁殖与呼吸综合征的免疫学研究进展[J].中国畜牧兽医,2010,37(8):194~198.
12 柴政,李曦,王小武,等.PRRSV高致病性毒株和经典毒株SYBR Green Ⅰ实时荧光PCR鉴别方法的建立[J].中国兽医科学,2009,39(2):140~144.
13 郭雅玮,宋杰,赵宝华.猪繁殖与呼吸综合征病毒对动物机体免疫机制的挑战[J].动物医学进展,2009,30(12):94~99.
14 梅林,赵建增,李真光,等.猪接PRRSV nsp-2△1882-2241弱毒株后血清细胞因子的变化特征[J].中国兽医学报,2012,32(3):361~366.
15 Andreyev V G,Wesley R D,Mengeling W L,et al.Genetic variation and phylogenetic relationships of 22 porcine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5[J].Archives of Virology,1997,142(5):993~1001.
16 Bautista E M,Molitor T W.IFN inhibits porcine reproductive and respiratory syndrome virus replication in macrophages[J].Arch Virol,1999,144(6):1191~1200.
17 Charerntantanakul W,Platt R,Johnson W,et al.Immune responses and protection by vaccine and various vaccine adjuvant candidates to virulent porcine reproductive and respiratory syndrome virus[J].Vet Immunol Immunopathol,2006,109(2):99~115.
18 Chen C,Cui S,Zhang C,et al.Development and validation of reverse transcription loop-mediated isothermal amplification for detection of PRRSV[J].Virus Genes,2010,40(1):76~83.
19 Dea S,Gagron C A,Mardassi H,et al.Antigenic variability among North American and European strains of porcine reproductive and respiratory syndrome as defined by monoclonal antibodies to the matrix protein[J].J Clin Microiol,1996,34(6):1488~1493.
20 Delputte P L,Meerts P,Costers S,et al.Effect of virus specific antibodies on attachment internalization and infection of porcine reproductive and respiratory syndrome virus in primary macrophages[J].Veterinary Immunology and Immunopathology,2004a,102(3):179~188.
21 Delputte P L,Nauwynck H J.Porcine arterivirus infection of alveolar macrophages is mediated by sialic acid on the virus[J].J Virol,2004b,78(15):8094~8101.
22 Fraile L,Calsamiglia M,Mateu E,et al.Prevalence of infection with porcine circovirus-2 (PCV-2) and porcine reproductive and respiratory syndrome virus (PRRSV) in an integrated swine production system experiencing post weaning multisystemic wasting syndrome[J].Can J Vet Res,2009,73(4):308~312.
23 Goldberg T L,Lowe J F,Milburn S M,et al.Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection[J].Virology,2003,317(2):197~207.
24 Kleiboeker S B,Schommer S K,Lee S M,et al.Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCR[J].J Vet Diagn Invest,2005,17(2):165~170.
25 Lopezo J,Oliveira M F,Garcia E A,et al.Protection against porcine reproductive and respiratory syndrome virus (PRRSV) infection through passive transfer of PRRSV-neutralizing antibodies is dose dependent[J].Clinical and Vaccine Immunology,2007,14(3):269~275.
26 Mardassi H,Mounir S,Dea S.Molecular analysis of the ORFs 3 to 7 of porcine reproductive and respiratory syndrome virus,Quebec reference strain[J].Archives of Virology,1995,140(8):405~418.
27 Meng X J,Paul P S,Halbur P G,et al.Sequence comparison of open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus[J].Journal of General Virology,1995,76(12):3181~3188.
28 Mori Y,Notomi T.Loop-mediated isothermal amplification (LAMP):A rapid,accurate,and cost-effective diagnostic method for infectious diseases[J].J Infect Chemother,2009,15(2):62~69.
29 North A,Gorbalenya A E.Virus encoded proteinases and proteolytic proceesing in the Nidovirales[J].J Gen Virol,2000,81(4):853~879.
30 Ohashi K,Burkart V,Flohe S,et al.Cutting edge:Heat shock portein 60 is a putative endogenous ligand of the toll like receptor 4 complex[J].J Immunol,2000,164(2):558~561.
31 Patterson A R,Johnson J,Ramamoorthy S,et al.Comparison of three enzyme-linked immunosorbent assays to detect porcine circovirus-2 (PCV-2)-specific antibodies after vaccination or inoculation of pigs with distinct PCV-1 or PCV-2 isolates[J].J Vet Diagn Invest,2008,20(6):744~751.
32 Sasaki K,Tsukahara T,Taira O,et al.Prevalence of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in piglets after weaning on a commercial pig farm in Japan[J].Anim Sci J,2010,81(1):135~141.
33 Shen S,Kwang J,Liu W,et al.Determination of the complete nucleotide sequence of a vaccine strain of porcine reproductive and respiratory syndrome virus and identification of the Nsp gene with a unique insertion[J].Arch Virol,2000,145(5):871~883.
34 Spilman M S,Welbon C,Nelson E,et al.Cryoelectrontomography of porcine reproductive and respiratory syndrome virus: Organization of the nucleocapsid[J].J Gen Virol,2009,90(3):527~535.
35 Suradhat S,Thanawongnuwec H R.Up regulation of interleukin-10 gene expression in the leukocytes of pigs infected with porcine reproductive and respiratory syndrome virus[J].Journal of General Virology,2003,84(10):2755~2760.
36 Thanawongnuwech R,Thacker B,Halbur P,et al.Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae[J].Clinical and Diagnostic Laboratory Immunology,2004,11(5):901~908.
37 Tian H,Wu J,Shang Y,et al.The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus[J].Virol J,2010,7(1):90.
38 Yoon K J,Zimmerman J,Swenson S L,et al.Characterization of the humoral immune response to porcine reproductive and respiratory syndrome(PRRS) virus infection[J].J Vet Diagn Invest,1995,7(3):305~312.
39 Zhou L,Zhang J,Zeng J,et al.The 30 amino acid deletionin the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence[J].J Virol,2009,83(10):5156~5167.

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