添加褪黑素对牛精液品质的影响

›› 2013, Vol. 40 ›› Issue (4): 177-179.

添加褪黑素对牛精液品质的影响

李晓霞, 曹平华, 禹学礼, 栗颖华

河南科技大学动物科技学院,河南洛阳 471003

收稿日期:2012-09-27 出版日期:2013-04-20 发布日期:2013-04-19
作者简介:李晓霞(1979-),女,河南人,博士,讲师,研究方向:哺乳动物的胚胎工程与性别控制。
基金资助:
河南科技大学博士科研启动基金资助(09001551);国家自然科学基金项目(31201801、31172207)。

The Effect of Melatonin on Semen Quality of Bovine

LI Xiao-xia, CAO Ping-hua, YU Xue-li, LI Ying-hua

College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Received:2012-09-27 Online:2013-04-20 Published:2013-04-19

摘要/Abstract

摘要： 为探讨褪黑素(melatonin,MLT)在牛精液保存中的抗氧化作用,本研究利用目测法、低渗膨胀法和考马斯亮兰染色法分析了不同MLT浓度(0、10^-3、10^-4和10^-5 mol/L)和不同孵育时间(1、4和8 h)对精子活力、质膜和顶体完整性的影响。结果发现,与对照组相比,在稀释液中分别添加10^-3和10^-4 mol/L MLT均可显著提高精子活力、质膜完整率和顶体完整率(P＜0.05),且10^-3 mol/L MLT添加效果最佳;MLT(10^-3 mol/L)组在27 ℃孵育1、4 h后,其精子的活力、质膜完整率和顶体完整率均显著高于对照组(P＜0.05)。因此,添加10^-3 mol/L MLT可明显改善牛精液品质,提高牛精液的保存时间和保存质量。

关键词: 褪黑素; 牛精液; 活力; 质膜完整率; 顶体完整率

Abstract: The antioxidative effect of melatonin (MLT) was evaluated on preserved bovine semen. Progressive motility (using visual observation), plasma membrane integrity (using the hypoosmotic swelling test, HOST) and acrosome integrity (using coomassie blue) of bovine spermatozoa were examined at 27 ℃ with 0, 10^-3, 10^-4, 10^-5 mol/L concentration and for 1, 4, 8 h incubation time. The results showed that progressive motility, the percentage of plasma membrane integrity and acrosome integrity of the bovine spermatozoa were higher for 10^-3 and 10^-4 mol/L MLT than that of the control group (P＜0.05). And progressive motility, the percentage of spermatozoa with plasma membrane integrity and acrosome integrity in melatonin (10^-3 mol/L ) groups at 1, 4 h were higher than that in the control group (P＜0.05). In conclusion, addition of optimal concentration of MLT helped to improve semen quality and life of preserved bovine semen.

Key words: melatonin; bovine semen; progressive motility; plasma membrane integrity; acrosome integrity

中图分类号:

S814.1

李晓霞, 曹平华, 禹学礼, 栗颖华. 添加褪黑素对牛精液品质的影响[J]. , 2013, 40(4): 177-179.

LI Xiao-xia, CAO Ping-hua, YU Xue-li, LI Ying-hua. The Effect of Melatonin on Semen Quality of Bovine[J]. , 2013, 40(4): 177-179.

参考文献

1 杜立银,曹少先,郭炜,等. 褪黑素在猪精液保存中的抗氧化作用[J]. 江苏农业学报,2009,25(2): 315~319.
2 Casao A, Mendoza N, Perez-Pe R,et al. Melatonin prevents capacitation and apoptotic-like changes of ram spermatozoa and increases fertility rate. J Pineal Res,2010, 48(1): 39~46.
3 Domínguez-Rebolledo A E, Fernández-Santos M R, Bisbal A,et al. Improving the effect of incu-bation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reproduction Fertility and Development,2010,22(5): 856~870.
4 Espino J, Bejarano I, Ortiz A,et al. Melatonin as a potential tool against oxidative damage and apoptosis in ejaculated human spermatozoa. Fertility and Sterility,2010, 94(5): 1915~1917.
5 Jang H Y, Kim Y H, Kim B W,et al. Ameliorative effects of melatonin against hydrogen peroxide-induced oxidative stress on boar sperm characteristics and subsequent in vitro embryo development. Reproduction in Domestic Animals,2010, 45(6):943~950.
6 Juknat A A, Méndez M A, Quaglino A,et al. Melatonin prevents hydrogen peroxide-induced Bax expression in cultured rat astrocytes. Journal of Pineal Research,2005, 38(2): 84~92.
7 Kumaresan A, Kadirvel G, Bujarbaruah K M,et al. Preservation of boar semen at 18 ℃ induces lipid peroxidation and apoptosis like changes in spermatozoa. Animal Reproduction Science,2009, 110(1~2): 162~171.
8 Li X X, Yang X G, Lu Y Q,et al. Protective effects of melatonin against oxidative stress in flow cytometry-sorted buffalo sperm. Reprod Domest Anim,2012, 47(2):299~307.
9 Okatani Y, Wakatsuki A, Kaneda C. Melatonin increases activities of glutathione peroxidase and superoxide dismutase in fetal rat brain. Journal of Pineal Research,2000, 28(2): 89~96.
10 Reiter R J, Tan D X, Osuna C, et al. Actions of melatonin in the reduction of oxidative stress: A review. J Biomed Sci,2000,7(6):444~458.
11 Reiter R J, Tan D X , Burkhardt S. Reactive oxygen and nitrogen species and cellular and organismal decline: Amelioration with melatonin[J]. Mech Ageing Dev, 2002, 123(8):1007~1019.
12 Rodriguez C, Mayo J C, Sainz R M,et al. Regulation of antioxidant enzymes: A significant role for melatonin. Journal of Pineal Research,2004, 36(1): 1~9.
13 Zehra K, Askin H, Faruk A,et al. Effect of melatonin on epididymal sperm quality alter testicular ischemia/reperfusion in rats. Fertility and Sterility,2010, 93(5): 1545~1549.

[1]	肖鹏, 尚江华, 杨春艳, 李孟琪, 段安琴, 马小娅, 冯超, 黄晨茜, 张博, 周金陈, 韦科龙, 郑威, 郑海英. α-亚麻酸对水牛卵巢颗粒细胞体外培养的影响[J]. 中国畜牧兽医, 2023, 50(6): 2380-2387.
[2]	李卫东, 蒋玉婷, 段宏伟, 何海军, 杨帅, 丁自强, 吴建新, 张恩秋, 胡俊杰. 褪黑素受体MT1和MT2在绵羊胃中的分布及表达[J]. 中国畜牧兽医, 2022, 49(8): 3256-3262.
[3]	郑海英, 杨春艳, 李玲玉, 唐丽萍, 郑威, 尚江华. 白藜芦醇对体外培养的水牛卵丘细胞增殖活力及其相关基因mRNA表达的影响[J]. 中国畜牧兽医, 2022, 49(1): 208-215.
[4]	马思佳, 刘媛, 汪序忠, 李亭亭, 段星, 杨松柏, 宋丹, 李向臣. 褪黑素对脂多糖刺激的滩羊骨骼肌卫星细胞炎性因子表达的影响[J]. 中国畜牧兽医, 2021, 48(9): 3378-3386.
[5]	吴昊, 姚松阳, 宋玉坤, 马慧, 郭刚, 王天坤, 李秀波, 路永强, 张鲁, 刘国世. 褪黑素对荷斯坦奶牛妊娠率及生殖激素的影响[J]. 中国畜牧兽医, 2021, 48(7): 2523-2529.
[6]	姚昱君, 李广栋, 吴昊, 马文奎, 杨海, 关盛宇, 吕东颖, 付瑶, 朱天奇, 姬鹏云, 谭鑫星, 赵万民, 连正兴, 张鲁, 刘国世. 白藜芦醇和褪黑素对绵羊胎儿成纤维细胞转染及卵母细胞体外成熟的影响[J]. 中国畜牧兽医, 2021, 48(12): 4497-4507.
[7]	徐妲, 罗晓彤, 陈璇, 吕艳秋, 金一. 活性氧对猪卵母细胞SUMO-1表达及其质量的影响[J]. 中国畜牧兽医, 2020, 47(8): 2544-2552.
[8]	李可可, 孙旭阳, 陈超, 荆焕松, 许学林, 游思, 赵靖波, 熊家军. 外源褪黑素对鹿茸软骨细胞生长发育的影响[J]. 中国畜牧兽医, 2020, 47(7): 1997-2004.
[9]	周乐乐, 李承德, 黄建豪, 陈云, 曾繁文, 黄晓刚, 张发良, 郑煦灿, 严常燕, 卫恒习, 张守全. 鸡精液稀释配方对低温保存精子活力和输精效果的影响[J]. 中国畜牧兽医, 2020, 47(6): 1837-1843.
[10]	王晓东, 杨婵, 刘清华, 李振宇, 吴昊, 刘国世, 李翔, 何长久. 山羊卵巢褪黑素信号特异性载体pcDNA3.1-Pro/GDF9-ASMT与pcDNA3.1-Pro/Cyp17-MTNR1A/B的构建[J]. 中国畜牧兽医, 2020, 47(12): 3833-3843.
[11]	薛俊欣, 熊炜, 张强, 黄忠荣, 李树清, 李春阳, 赵新宇, 李健, 王权. 4种有机溶剂对Vero细胞活力和弓形虫增殖的影响[J]. 《中国畜牧兽医》, 2019, 46(8): 2265-2272.
[12]	黄子妍, 孙研研, 李云雷, 许红, 刘一帆, 石雷, 叶建华, 麻慧, 倪爱心, 王攀林, 谢金防, 陈继兰. 鸡lncRNA-MSTRG.15568.9及其预测靶基因的表达[J]. 《中国畜牧兽医》, 2019, 46(7): 1986-1993.
[13]	赵芳, 王根, 赵国栋, 高超, 李晓斌, 马晨, 杨开伦. 瘤胃保护性5-羟基色氨酸对绵羊肠道内容物褪黑素含量及菌群结构的影响[J]. 《中国畜牧兽医》, 2019, 46(3): 690-702.
[14]	李芳芳, 龙开旭, 俸祥仁, 黄项生, 潘堂峰, 江明生, 王国利, 邓祝新, 蒋钦扬, 郭亚芬. 河流型水牛热休克蛋白70的原核表达及其对精液抗冻性的影响[J]. 《中国畜牧兽医》, 2018, 45(9): 2417-2424.
[15]	宋慧子, 边小娜, 栗瑞兰, 刘春洁, 张家新. 绵羊附睾褪黑素受体1的表达与定位[J]. 《中国畜牧兽医》, 2018, 45(9): 2544-2549.