›› 2013, Vol. 40 ›› Issue (10): 203-206.

• 疾病防治 • 上一篇    下一篇

猪肉和牛奶源葡萄球菌耐药性调查

魏鸿堃1, 冯言言1, 刘健华1, 吕世明2, 曾振灵1   

  1. 1. 华南农业大学兽医学院, 广东广州 510642;
    2. 贵州大学动物科学学院, 贵州贵阳 550025
  • 收稿日期:2013-03-21 出版日期:2013-10-20 发布日期:2013-12-19
  • 作者简介:魏鸿堃(1987-),男,浙江人,硕士生,研究方向:兽医药理与毒理学。
  • 基金资助:
    高等学校博士学科点专项科研(20104404110008)。

Survey on Antimicrobial Susceptibility of Staphylococcus Isolated from Pork and Milk

WEI Hong-kun1, FENG Yan-yan1, LIU Jian-hua1, LV Shi-ming2, ZENG Zhen-ling1   

  1. 1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
    2. College of Animal Science, Guizhou University, Guiyang 550025, China
  • Received:2013-03-21 Online:2013-10-20 Published:2013-12-19

摘要: 2010年从贵州省猪肉和牛奶样品中分离鉴定出73株葡萄球菌,其中金黄色葡萄球菌50株,凝固酶阴性葡萄球菌23株。采用琼脂稀释法检测其对18种抗菌药物的敏感性,运用统计学方法比较分析金黄色葡萄球菌与凝固酶阴性葡萄球菌的耐药性差异。结果显示,动物性食品源葡萄球菌耐药较严重,对临床上常用药物耐药率较高,且为多重耐药,其中乳源葡萄球菌的多重耐药现象较猪肉源葡萄球菌严重;猪肉源金黄色葡萄球菌主要对青霉素类、四环素和大环内酯类耐药,乳源金黄色葡萄球菌主要对青霉素类、头孢菌素类、大环内酯类、四环素、克林霉素、泰妙菌素及利福平耐药;猪肉源凝固酶阴性葡萄球菌主要对氨苄西林、苯唑西林和泰妙菌素耐药,乳源凝固酶阴性葡萄球菌的耐药谱与乳源金黄色葡萄球菌类似。

关键词: 金黄色葡萄球菌; 凝固酶阴性葡萄球菌; 耐药性; 猪肉; 牛奶

Abstract: 73 Staphylococcus strains including 50 Staphylococcus aureus (S.aureus) and 23 coagulase-negative Staphylococci were isolated from pork and milk samples with collected from Guizhou province in 2010. Antimicrobial susceptibility testing was performed with agar dilution method according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). Statistical methods were used to analyze the differences of antimicrobial susceptibility between S.aureus and coagulase-negative Staphylococci which isolated from different samples. The results showed that these isolates displayed a high degree of resistance to the common antimicrobials and showed multi-drug resistance. Moreover, isolates which isolated from the milk samples showed much higher multi-drug resistance than those isolated from the pork samples. S.aureus which isolated from the pork samples demonstrated primarily resistant to penicillins,tetracyclines and macrolides,while S.aureus which isolated from the milk samples showed primarily resistant to penicillins,cephalosporins, macrolides, tetracyclines, clindamycin, tiamulin and rifampicin. The coagulase-negative Staphylococci which isolated from the pork samples displayed primarily resistant to ampicillin,oxacillin and tiamulin, and the resistance profile of coagulase-negative Staphylococci from milk samples showed no remarkable difference from that of the S.aureus isolated from the milk samples.

Key words: Staphylococcus aureus; coagulase-negative Staphylococci; resistance; pork; milk

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