›› 2012, Vol. 39 ›› Issue (7): 70-73.

• 生物技术 • 上一篇    下一篇

ACVR1基因对京海黄鸡生长和繁殖性状的遗传效应研究

王文浩1, 王金玉1, 张跟喜1, 赵秀华1, 俞亚波2, 施会强2   

  1. 1. 扬州大学动物科学与技术学院,江苏扬州 225009;
    2. 江苏京海集团,江苏南通 226103
  • 收稿日期:2011-12-30 出版日期:2012-07-20 发布日期:2012-07-16
  • 通讯作者: 王金玉,男,江苏人,教授,博导,从事动物遗传育种的研究。Tel:0514-87979075;E-mail:jywang@yzu.edu.cn E-mail:jywang@yzu.edu.cn
  • 作者简介:王文浩(1988-),男,江苏人,硕士生,从事动物遗传育种的研究。
  • 基金资助:
    国家肉鸡产业技术体系资助(nycytx-42-G1-05);江苏高校优势学科建设工程资助。

Genetic Effects of ACVR1 Gene on Growth and Reproductive Performance in Jinghai Yellow Chicken

WANG Wen-hao1, WANG Jin-yu1, ZHANG Gen-xi1, ZHAO Xiu-hua1, YU Ya-bo2, SHI Hui-qiang2   

  1. 1. Department of Animal Science,Yangzhou University,Yangzhou 225009,China;
    2. Jiangsu Jinghai Poultry Group Co., Ltd., Nantong 226103,China
  • Received:2011-12-30 Online:2012-07-20 Published:2012-07-16

摘要: 以京海黄鸡为材料,采用PCR-SSCP和DNA测序技术对ACVR1基因外显子多态性进行研究。结果表明,ACVR1基因20190 bp处发生G→A突变,检测到AA、AB和BB 3种基因型。A等位基因频率为0.9075,B等位基因频率为0.0925。基因型与生长和繁殖性状的关联性分析发现,BB基因型鸡的开产体重极显著高于AA基因型鸡(P<0.01),AA基因型鸡300日龄体重显著高于BB基因型鸡(P<0.05)。由此初步推断,ACVR1基因可能对京海黄鸡的产蛋和发育有一定的影响,A为体重的优势基因,B为产蛋的优势基因。

关键词: 京海黄鸡; ACVR1基因; 多态位点

Abstract: In this study, Jinghai Yellow chicken was used to analyze ACVR1 gene polymorphisms by PCR-SSCP and DNA sequencing method. As a result, there was one mutation site(G20190A) in Jinghai Yellow chicken. Three genotypes were detected(AA, AB, BB), and the frequencies of allele A and B were 0.9075 and 0.0925. For the reproductive performance, BB genotype in open weight was significantly higher than AA genotype (P<0.01), however AA genotype in 300 days of age weight was significantly higher than BB genotype (P<0.05). It implied that ACVR1 gene might have a fixed effect on growth and reproductive effect in Jinghai Yellow chicken.

Key words: Jinghai Yellow chicken; ACVR1 gene; polymorphisma

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