猪圆环病毒2型10JS-2株的分离与全基因组序列分析

›› 2012, Vol. 39 ›› Issue (7): 6-10.

猪圆环病毒2型10JS-2株的分离与全基因组序列分析

蔡林¹, 胡冬梅¹, 李晓霞¹, 汪葆玥¹, 韩雪¹, 倪建强¹, 周智¹, 遇秀玲¹, 翟新验¹, 王文良², 田克恭¹

1. 中国动物疫病预防控制中心,北京 100026;
2. 军事医学科学院,北京 100071

修回日期:2012-04-05 出版日期:2012-07-20 发布日期:2012-07-16
通讯作者: 田克恭(1964－),男,研究员,从事动物疫病诊断与防控技术研究。E-mail:tiankg@263.net王文良(1965－),男,实验师,从事实验动物管理与检测技术研究。Tel:010-66948345 E-mail:tiankg@263.net
作者简介:蔡林(1987－),男,山东人,硕士,研究方向:动物疫病诊断。
基金资助:
北京市生猪产业技术体系创新团队(GWZJ-2009-05)。

Isolation and Complete Genome Sequence Analysis of Porcine Circovirus Type 2 (PCV2) Strain 10JS-2 from Jiangsu Province, China

CAI Lin¹, HU Dong-mei¹, LI Xiao-xia¹, WANG Bao-yue¹, HAN Xue¹, NI Jian-qiang¹, ZHOU Zhi¹, YU Xiu-ling¹, ZHAI Xin-yan¹, WANG Wen-liang², TIAN Ke-gong¹

1. China Animal Disease Control Centre, Beijing 100026, China;
2. Academy of Military Medical Science, Beijing 100071, China

Revised:2012-04-05 Online:2012-07-20 Published:2012-07-16

摘要/Abstract

摘要： 本试验从江苏某猪场采集猪血清,进行猪圆环病毒2型(PCV2)的检测和分离,根据GenBank中登录的PCV2全基因组序列,设计1对特异性引物,扩增PCV2全基因组,并且进行序列测定和分析。发现一株PCV2 10JS-2的基因组全长为1779 nt,在病毒复制起始区域含有11个碱基的插入。在GenBank中对含有11个碱基插入的毒株序列进行BLAST发现,有两株PCV2(AY321993和EF565360)也有11个碱基的插入,但是与10JS-2比较,插入的碱基和插入的位置不同。遗传进化分析显示10JS-2属于PCV2a基因型,AY321993和EF565360属于PCV2b基因型,因此推断10JS-2可能是由PCV2a基因型的毒株在复制起始区域发生碱基插入形成的。这是首次发现此类PCV2毒株。

关键词: 猪圆环病毒2型; 分离; 全基因组序列; 序列分析

Abstract: Serum sample was collected from a pig in Jiangsu province,China,and virus detection and isolation of porcine circovirus type 2 (PCV2) were performed. A pair of primers was designed to amply the complete PCV2 genome according the PCV2 sequences submitted to GenBank for sequencing and analysis. A PCV2 strain 10JS-2 was isolated. The complete genomic sequence of 10JS-2 was 1779 nt in length. Remarkably,a special 11 nt insertion in the origin of viral genome replication (Ori) of 10JS-2 was observed. BLAST search on NCBI website revealed that another two PCV2 strains (AY321993 and EF565360) also had 11 nt insertion in Ori. However,the insertion sequence and the location of the insertion were different. Phylogenetic analysis revealed that 10JS-2 belong to genotype PCV2a,while AY321993 and EF565360 were classified into genotype PCV2b. So,we inferred that 10JS-2 probably derived from PCV2a strains that obtained the 11 nt insertion in Ori. This is the first report about this kind of PCV2a strain.

Key words: porcine circovirus type 2; isolation; complete genome sequence; sequence analysis

中图分类号:

蔡林, 胡冬梅, 李晓霞, 汪葆玥, 韩雪, 倪建强, 周智, 遇秀玲, 翟新验, 王文良, 田克恭. 猪圆环病毒2型10JS-2株的分离与全基因组序列分析[J]. , 2012, 39(7): 6-10.

CAI Lin, HU Dong-mei, LI Xiao-xia, WANG Bao-yue, HAN Xue, NI Jian-qiang, ZHOU Zhi, YU Xiu-ling, ZHAI Xin-yan, WANG Wen-liang, TIAN Ke-gong. Isolation and Complete Genome Sequence Analysis of Porcine Circovirus Type 2 (PCV2) Strain 10JS-2 from Jiangsu Province, China[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(7): 6-10.

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