›› 2012, Vol. 39 ›› Issue (6): 242-244.

• 经验交流 • 上一篇    下一篇

高致病性猪繁殖与呼吸综合征的调查报告

栾智华, 周洪臣   

  1. 辽宁省盘锦市动物疫病预防控制中心,辽宁盘锦 124010
  • 修回日期:2011-12-16 出版日期:2012-06-20 发布日期:2012-07-02
  • 作者简介:栾智华(1979-),女,河北人,博士生,兽医师,研究方向:重大动物疫病诊断与防控技术研究。

Investigation on Highly Pathogenic Porcine Reproductive and Respiratory Syndrome

LUAN Zhi-hua, ZHOU Hong-chen   

  1. Panjin Center for Prevention and Control of Animal Epidemic Disease, Panjin 124010,China
  • Revised:2011-12-16 Online:2012-06-20 Published:2012-07-02

摘要: 为了解2011年盘锦地区免疫猪群中高致病性猪繁殖与呼吸综合征的免疫效果和感染状况,采用ELISA和荧光定量RT-PCR方法分别检测了本年度1-12月采集到的2160份猪血清和120份猪组织样品。结果表明,受检的猪组织样品中均无高致病性猪繁殖与呼吸综合征抗原;大洼县、盘山县、兴隆台区、双台子区猪繁殖与呼吸综合征抗体阳性率分别为82.2%、81.1%、78.8%、81.1%;种猪场、商品代猪场、散养户的猪繁殖与呼吸综合征抗体阳性率分别为90%、81.3%、75%。从总体来看,2011年盘锦地区的猪繁殖与呼吸综合征免疫状况良好,无猪繁殖与呼吸综合征疫情发生。

关键词: 高致病性猪繁殖与呼吸综合征; 酶联免疫吸附试验; 荧光RT-PCR

Abstract: To investigate the infection status and the immune effect of the highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) from immunized pig populations of Panjin city in 2011, 2160 serum samples and 120 tissue specimens were collected from pig farms in Panjin city from January to December in 2011, and examined by enzyme linked immunosorbent assay (ELISA) and quantitative real-time PCR respectively. The results showed that there were not the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-RRSV) in the tissues, the antibodies positive rate in Dawa,Panshan, Xinglongtai,Shuangtaizi was 82.2%, 81.1%,78.8%,81.1%, respectively; the antibodies positive rate in swine breeding farm,commercial pig farm,peasant household pigs was 90%,81.3%,75%, respectively. In summary, the immune status of HP-PRRS was fine, HP-PRRS did not break out in this year.

Key words: HP-PRRS; ELISA; quantitative real-time PCR

中图分类号: