黑色素皮质激素受体1基因248位点多态性与中国马品种毛色的相关性分析

›› 2012, Vol. 39 ›› Issue (6): 149-152.

黑色素皮质激素受体1基因248位点多态性与中国马品种毛色的相关性分析

党珍^1,3, 王嘉福¹, 赵星艳¹, 黄勇^1,2, 田松军^1,2, 王荣明², 冉雪琴¹

1. 贵州大学动物科学学院,贵州贵阳 550025;
2. 贵州安顺紫云县畜牧兽医局,贵州紫云 561600;
3. 重庆市公安局,重庆 404100

收稿日期:2011-12-06 出版日期:2012-06-20 发布日期:2012-07-02
通讯作者: 冉雪琴,教授,研究方向:动物生理与分子生物学。Tel: 0851-3863510;Fax:0851-8298003;E-mail:as.xqran@gzu.edu.cn E-mail:as.xqran@gzu.edu.cn
作者简介:党珍(1984－),女,山东人,硕士生,研究方向:动物生理与分子生物学。
基金资助:
贵州省农业攻关项目(黔科合NY字2009-3068);贵州省农业动植物育种专项(黔农育专字2009-021号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006)。

Association of Polymorphism in MC1R Gene at Site 248 with Chestnut Coat Color in Three Chinese Horse Breeds

DANG Zhen^1,3, WANG Jia-fu¹, ZHAO Xing-yan¹, HUANG Yong^1,2, TIAN Song-jun^1,2, WANG Rong-ming², RAN Xue-qin¹

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Animal Husbandry and Veterinary Bureau of Ziyun County, Ziyun 561600, China;
3. Chongqing Police Bureau, Chongqing 404100, China

Received:2011-12-06 Online:2012-06-20 Published:2012-07-02

摘要/Abstract

摘要： 在欧洲马品种中,黑色素皮质激素受体1(melanocortin receptor 1,MC1R)基因第248位碱基有C/T多态性,纯合的T248位点决定欧洲马的栗毛色。针对MC1R基因的248位点设计了2对特异性引物,采用等位基因特异性PCR技术,研究3个中国马品种MC1R基因型与栗色毛之间的关系。经扩增获得两种DNA片段,克隆测序后证明,扩增片段确为MC1R基因,两种DNA片段序列在基因的248位点的确呈现C/T多态性,但检测126份贵州矮马、西南马和新疆伊犁马血液样本,全部为杂合基因型(Ee),其中包括栗毛、黑毛、骝毛3种单毛色及3种复毛色。这些研究结果提示,MC1R基因中248位点的多态性与国内3个马品种的栗色毛之间没有直接的相关性。

关键词: 贵州矮马; 西南马; 伊犁马; 毛色; MC1R基因

Abstract: A single nucleotide polymorphism (SNP) at site 248 of melanocortin receptor 1 gene (MC1R) is known from European horse breeds, in which the homozygous genotype (ee) is related with the chestnut coat color of European horse individuals. To confirm the polymorphism of site 248 of MC1R gene in Chinese horse, three horse breeds of Guizhou pony, Southwest horse and Ili horse were chosen as samples with coat color of three monochromatic (chestnut, black, bay) and three kinds of complex coat color. Two pairs specific primers were designed. Two fragments, 124 and 481 bp, were amplificated by allele-specific polymerase chain reaction (AS-PCR) method. The polymorphism of site 248 in MC1R gene was further approved by cloning and sequencing of the DNA fragments. The results showed that genotypes of all samples with coat color of chestnut were heterozygous genotype (Ee). However, the genotypes of other samples were Ee either, in which the coat colors included bay, black, grey, spotted but not chestnut. It suggested that the SNP at site 248 of MC1R gene might be unrelated with the chestnut coat color in native Chinese horse breeds.

Key words: Guizhou pony; Southwest horse; Ili horse; coat color; MC1R gene

中图分类号:

S821

党珍, 王嘉福, 赵星艳, 黄勇, 田松军, 王荣明, 冉雪琴. 黑色素皮质激素受体1基因248位点多态性与中国马品种毛色的相关性分析[J]. , 2012, 39(6): 149-152.

DANG Zhen, WANG Jia-fu, ZHAO Xing-yan, HUANG Yong, TIAN Song-jun, WANG Rong-ming, RAN Xue-qin. Association of Polymorphism in MC1R Gene at Site 248 with Chestnut Coat Color in Three Chinese Horse Breeds[J]. , 2012, 39(6): 149-152.

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