›› 2012, Vol. 39 ›› Issue (2): 191-193.

• 疾病防治 • 上一篇    下一篇

青海地区奶牛无乳链球菌的分离与鉴定

杨有武   

  1. 青海省海北州海晏县兽医站,青海海北 812200
  • 收稿日期:2011-06-02 修回日期:1900-01-01 出版日期:2012-02-20 发布日期:2012-02-20

Isolation and Identification of Streptococcus agalactiae from Dairy Cow Mastitis in Qinghai Province

YANG You-wu   

  1. Haiyan County Veterinary Station of Qinghai Province, Haibei 812200, China
  • Received:2011-06-02 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

摘要: 无乳链球菌是导致奶牛乳房炎的常见病原菌之一,为了掌握青海地区无乳链球菌的流行情况及常用药物的敏感性,从而为本地区更好的预防与治疗奶牛乳房炎提供保障。本试验在青海地区16个奶牛场共采集258份乳房炎乳样,经革兰氏染色与绵羊鲜血琼脂培养基培养纯化,获得疑似链球菌137株;随后经玻片法血浆凝固酶试验、CAMP试验、生化试验与动物致病性试验对无乳链球菌进行鉴定,结果显示,137株链球菌中含有无乳链球菌42株,分离率为30.7%;经K-B纸片扩散法对无乳链球菌耐药性检测结果显示,该地区无乳链球菌主要对头孢类高度敏感,对氧氟沙星、磺胺类、氨苄青霉素与阿米卡星中度敏感。

关键词: 奶牛; 乳房炎; 无乳链球菌; 分离鉴定

Abstract: Streptococcus agalactiae is one of pathogenic bacterium that inducing mastitis of dairy cow. In order to master the popularity and laid the reference for selection of medicine to treat mastitis, 258 samples were collected from 16 dairy cow farms in Qinghai province, and 137 Streptococcus strains were isolated,including Streptococcus agalactiae 42 strains after sub-culture, gram straining, plasma-coagulase test, CAMP test, biochemical tests and animal pathogenicity tests. The results indicated that Streptococcus agalactiae was the primary pathogenic bacteria of mastitis. All of Streptococcus agalactiaes were hypersensitive to cephradine, and partially resistant to amikacin ofloxacin, ampicillin and sulfanilamide group after K-B medicine sensitivity test.

Key words: dairy cow; mastitis; Streptococcus agalactiae; isolation and identification

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