›› 2012, Vol. 39 ›› Issue (12): 117-121.

• 遗传繁育 • 上一篇    下一篇

文昌鸡促卵泡激素β基因内含子1的SNP分析及其与早期产蛋性能的相关性研究

王青娟1, 李同明1, 尹逊河1, 骆兰平2, 王慧1   

  1. 1. 山东农业大学动物科技学院,山东泰安 271018;
    2. 德州市农业局,山东德州 253016
  • 收稿日期:2012-05-18 出版日期:2012-12-20 发布日期:2012-12-19
  • 通讯作者: 王慧,教授。E-mail:wanghui2328@sdau.edu.cn E-mail:wanghui2328@sdau.edu.cn
  • 作者简介:王青娟(1984-),女,山东人,硕士生,主要从事动物形态学与神经发育学研究。
  • 基金资助:
    山东省农业良种工程项目(2011LZ15-4)。

Single Nucleotide Polymorphisms of FSHβ Gene Intron 1 and Association with Early Egg Production Traits in Wenchang Chicken

WANG Qing-juan1, LI Tong-ming1, YIN Xun-he1, LUO Lan-ping2, WANG Hui1   

  1. 1. College of Animal Scinece and Technology, Shandong Agricultural University,Tai’an 271018,China;
    2. Agriculture Bureau of Dezhou City,Dezhou 253016,China
  • Received:2012-05-18 Online:2012-12-20 Published:2012-12-19

摘要: 以109只海南文昌鸡为试验动物,利用PCR-SSCP技术检测了促卵泡激素(follicle-stimulating hormone β,FSHβ)基因的多态性,在其内含子1区域内发现了1个SNP,在群体中检测出2种基因型:AA、AB。将不同基因型个体与产蛋数、畸形蛋数、体重和开产日龄等进行了关联分析。结果表明,AA基因型鸡个体的产蛋数比AB基因型个体多9.54枚,畸形蛋数比AB基因型个体少1.54枚,开产日龄比AB基因型个体早2.97 d,且差异均不显著(P>0.05)。22~26、27~30和31~34周龄的AA基因型鸡的产蛋量显著高于AB基因型(P<0.05),而18~21和35~39周龄的AA基因型鸡的产蛋量也明显高于AB基因型,但差异不显著(P>0.05)。AA基因型鸡6、8、14、20周龄的体重高于AB基因型个体,AA基因型个体18周龄的体重却低于AB基因型个体,差异均不显著(P>0.05)。总体而言,FSHβ基因位点的A等位基因对产蛋数、畸形蛋数、体重和开产日龄的影响均未达到差异显著水平。

关键词: 文昌鸡; PCR-SSCP; SNP; FSHβ基因

Abstract: This study detected gene polymorphisms of follicle-stimulating hormone β (FSHβ),in which 1 SNP in intron 1 region was found,and 2 genotypes were detected,respectively,for genotype AA and AB by means of PCR-SSCP technique with 109 Wenchang chickens in Hainan as experimental animals. To conduct correlation analysis among different genotypes and egg production (NE),the number of abnormal eggs (ENA),body weight (BW),and age at first egg (AFG),the results showed that NE of AA genotype chicken individual was more 9.54 than that of AB genotype;ENA of AA genotype was less 1.54 than that of AB genotype;AFG of AA genotype was 2.97 days earlier than that of AB genotype,and the difference was not significantly (P>0.05). At 22 to 26, 27 to 30 and 31 to 34 weeks of age, the egg production of AA genotype chicken was obviously higher than that of the AB genotype, and the difference was significantly(P<0.05).At 18 to 21 and 35 to 39 weeks of age, the egg production of AA genotype chicken also was obviously higher than that of the AB genotype, but the difference was not significantly(P>0.05).BW of AA genotype was higher than that of AB genotype at 6,8,14,20 weeks of age,but BW of AA genotype was higher than that of AB genotype at 18 weeks of age,both of the differences were not significantly (P>0.05). In general,A allele of the FSHβ gene did not significantly impact on NE,ENA,BW and AFG.

Key words: Wenchang chickens; PCR-SSCP; SNP; FSHβ gene

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