›› 2011, Vol. 38 ›› Issue (5): 143-145.

• 疾病防治 • 上一篇    下一篇

1株鸭源新城疫病毒的分离鉴定及生物学特性研究

刘大伟, 焦培荣, 黎先伟, 何琴, 高诗敏, 漆丹丹, 陈德成, 任涛   

  1. 华南农业大学兽医学院,农业部动物疫病防控重点开放实验室, 广东省动物源性人兽共患病预防与控制重点实验室,广东广州 510642
  • 收稿日期:2010-10-27 修回日期:1900-01-01 出版日期:2011-05-20 发布日期:2011-05-20
  • 通讯作者: 任涛

Identificationand Biological Characterization of a Newcastle Disease Virus from Duck

LIU Da-wei, JIAO Pei-rong, LI Xian-wei, HE Qin, GAO Shi-min, QI Dan-dan, CHEN De-cheng, REN Tao   

  1. Key Lab of Zoonoses Control and Prevention of Guangdong,College of Veterinary Medicine,Key Lab of Animal Disease Control and Prevention of Ministry of Agriculture,South China Agricultural University,Guangzhou 510642,China
  • Received:2010-10-27 Revised:1900-01-01 Online:2011-05-20 Published:2011-05-20

摘要: 从广东地区发病鸭中分离到1株新城疫病毒,血凝试验、血凝抑制试验结果表明此分离株具有血凝活性,且这种血凝性可被NDV标准阳性血清抑制,而不能被AIV-H5、AIV-H7、AIV-H9、EDSV阳性血清抑制;用针对NDV F基因特异性鉴定引物对该分离株进行聚合酶链反应扩增,可扩增出相应的目的片段。由此,初步鉴定该分离株为鸭源新城疫病毒,并命名为NDV/DUCK/SD/2006。另外,通过毒力测定,该毒株的MDT、IVPI分别为51.75 h、2.44,判定为强毒力株。

关键词: 新城疫病毒; 鸭; 广东; 生物学特性

Abstract: A Newcastle disease virus(NDV) was isolated from duck in Guangdong. It was identified with hemagglutination,hemagglutination inhibition. These results showed the isolate had hemagglutination and was inhibited by the standard antibody of AIV-H5,AIV-H7,AIV-H9 and EDSV. It was amplified by RT-PCR and sequenced,which was used by the specific identification primers of NDV F gene. Thus,the isolate was identified as a duck NDV,named NDV/DUCK/SD/2006. In this research,the isolate were conducted virulence determination. Results showed that,the MDT,and IVPI of the isolate were 51.75 h,2.44 respectively,suggesting it was a velogenic strain.

Key words: NDV; duck; Guangdong; biological characterization

中图分类号: