›› 2011, Vol. 38 ›› Issue (12): 188-190.

• 疾病防治 • 上一篇    下一篇

肉鹅大肠杆菌病的病原分离与鉴定

徐永荣1, 陈宏生2, 赵宝华2   

  1. 1. 江苏省兴化市动物卫生监督所,江苏兴化 225700;2. 中国农业科学院家禽研究所,江苏扬州 225003
  • 收稿日期:1900-01-01 修回日期:2011-06-07 出版日期:2011-12-20 发布日期:2011-12-20
  • 通讯作者: 赵宝华

Isolation and Identification of Escherichia coli from Geese

XU Yong-rong1, CHEN Hong-sheng2, ZHAO Bao-hua2   

  1. 1. Xinghua Animal Health Inspection Institute, Xinghua 225700, China;2. Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225003, China
  • Received:1900-01-01 Revised:2011-06-07 Online:2011-12-20 Published:2011-12-20

摘要: 本试验旨在研究肉鹅大肠杆菌的生化特性、药敏情况、血清型和致病性。2010年12月江苏省某肉鹅场鹅群出现呼吸困难,死亡增多现象,对病死鹅剖检发现以气囊炎和肺部坏死为主,无菌采集病料分离到一株细菌,经分离培养、染色镜检、生化特性鉴定,确定为大肠杆菌,微量凝集试验表明其血清型为O2。经过肺结节压片镜检,没有发现霉菌的菌丝;检测健康鹅和患病鹅各10只的鹅新城疫抗体,发现其抗体滴度比较高且整齐度较好,两者之间的抗体无明显差异。对分离菌株进行药敏试验,结果表明对氟苯尼考等7种药物敏感,对先锋Ⅴ等16种药物已产生了耐药性。将分离到的菌株接种21日龄雏鹅进行动物回归试验,引起80%死亡率,说明该分离菌株对雏鹅具有较强的致病性。

关键词: 鹅; 大肠杆菌; 血清型; 药敏试验; 致病性试验

Abstract: The purpose of this paper is to study biochemical characteristics, drug sensitivity, serotype and pathogenicity of Escherichia coli. The geese from a farm in Jiangsu province had a symptom of dyspnea and high mortality. The autopsy shows air sac inflammation and pulmonary necrosis. One strain was isolated, cultured, stained and tested by microscopy, biochemical identified. The result showed that the isolate was Escherichia coli, agglutination test showed that its serotype was O2. After microscopic examination of lung nodules tablet, fungal hyphae was no found. 10 sick pigeon and 10 health pigeons were detected respectively for Newcastle disease antibodies, the result demonstrated that the titre was high and uniformity was good and there was no difference between the two antibodies. Sensitive test showed that 7 kinds of drugs such as Florfenicol were sensitive and other 16 drugs such as Cefamezin had drug resistance. The isolated strains were injected 21 days old pigeons, caused 80% mortality. The results show that the isolate has strong pathogenicity.

Key words: geese; Escherichia coli; serotype; drug sensitive test; pathogenicity

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