牛磺鹅去氧胆酸对热应激小鼠免疫功能的调节作用

›› 2011, Vol. 38 ›› Issue (11): 47-50.

牛磺鹅去氧胆酸对热应激小鼠免疫功能的调节作用

夏俊峰¹, 李金莲², 李培锋³, 王丛丛¹, 王振勇¹, 石有斐¹

1. 山东农业大学动物科技学院,山东泰安 271018;2. 泰山学院生物科学与技术系,山东泰安 271021;3. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018

收稿日期:2011-04-06 修回日期:1900-01-01 出版日期:2011-11-20 发布日期:2011-11-20
通讯作者: 石有斐,王振勇

The Regulating Effects of Taurochenodeoxycholic Acid on Immune Function in Heat-stressed Mice

XIA Jun-feng¹, LI Jin-lian², LI Pei-feng³, WANG Cong-cong¹, WANG Zhen-yong¹, SHI You-fei¹

1. College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai'an 271018,China;2. Department of Biological Science and Technology,Taishan University,Tai'an 271021,China;3. College of Animal Science and Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2011-04-06 Revised:1900-01-01 Online:2011-11-20 Published:2011-11-20

摘要/Abstract

摘要： 为探讨牛磺鹅去氧胆酸(taurochenodeoxycholic acid,TCDCA)对热应激小鼠免疫功能的调节作用,试验将50只小鼠随机分成5组,分别为对照组、热应激组和低、中、高剂量TCDCA组(剂量分别为50、100、200 mg/kg)。热应激组和低、中、高剂量TCDCA组小鼠置于(35.5±0.5) ℃、相对湿度60%环境下连续热应激7 d。结束后分别检测小鼠脾脏指数、血清IgG、小肠sIgA的含量及外周血T淋巴细胞亚群和B淋巴细胞的变化。结果表明,热应激引起小鼠脾脏指数显著下降(P＜0.05),血清IgG、肠sIgA显著升高(P＜0.05),外周血CD8⁺、CD19⁺百分率显著下降(P＜0.05),CD4⁺百分率下降不显著(P＞0.05),CD4⁺/CD8⁺比值显著升高(P＜0.05),表明热应激导致小鼠机体免疫功能发生紊乱。TCDCA对热应激小鼠脾脏指数无显著影响;但能显著抑制热应激引起的血清IgG含量、小肠sIgA含量升高(P＜0.05)和显著提高外周血CD8⁺、CD19⁺百分率(P＜0.05),同时能够调节CD4⁺/CD8⁺ 比值到正常水平。因此,TCDCA对热应激导致的小鼠免疫功能紊乱具有显著的改善作用。

关键词: 牛磺鹅去氧胆酸; 热应激; 免疫调节

Abstract: To investigate regulating effects of taurochenodeoxycholic acid (TCDCA) on immune function in mice under heat stress,50 Kunming mice were divided randomly into five groups: normal control group,heat stress group,TCDCA1 group (50 mg/kg),TCDCA2 group (100 mg/kg),and TCDCA3 group (200 mg/kg). The mice of normal control group were fed in the temperature of 22 ℃,and the mice of other groups were treated with temperature of 35.5 ℃±0.5 ℃ and 60% humidity for 7 days. The spleen index,the change of T cell subgroup and B lymphocyte of peripheral blood,the contents of intestinal sIgA and serum IgG in mice were measured. The results showed that spleen index in mice of heat stress group was significantly lowered compared to the normal control group(P＜0.05);the contents of blood serum IgG and Intestinal sIgA were significantly (P＜0.05) higher;the percentage of CD8⁺ and CD19⁺ lymphocytes decreased significantly (P＜0.05),and the CD4⁺/CD8⁺ value increased significantly (P＜0.05),which demonstrated that the immune functions of mice under heat stress falls into disorders. The results also indicated that TCDCA have no effects on spleen index in heat stress,TCDCA enhanced the percentage of CD8⁺ and CD19⁺ lymphocytes in peripheral blood remarkably (P＜0.05),TCDCA recovered the CD4⁺/CD8⁺ value,remarkably and TCDCA decreased concentration of IgG in blood serum and sIgA in intestinal remarkably (P＜0.05). These findings suggested that the immunity of mice in heat-stress could be destroyed and leaded to disorder,and can be accumulated by TCDCA.

Key words: taurochenodeoxycholic acid; heat stress; immunological regulation

中图分类号:

S852.4

夏俊峰;李金莲;李培锋;王丛丛;王振勇;石有斐. 牛磺鹅去氧胆酸对热应激小鼠免疫功能的调节作用[J]. , 2011, 38(11): 47-50.

XIA Jun-feng;LI Jin-lian;LI Pei-feng;WANG Cong-cong;WANG Zhen-yong;SHI You-fei. The Regulating Effects of Taurochenodeoxycholic Acid on Immune Function in Heat-stressed Mice[J]. , 2011, 38(11): 47-50.

[1]	孙鹏飞, 王晓涵, 胡云, 吴慧光, 赵静雯, 罗绪刚. 热应激对鸡生产性能、血清生化和肠道微生物影响研究进展[J]. 中国畜牧兽医, 2024, 51(2): 581-590.
[2]	冯安妮, 李其龙, 张义伟, 桑晓宇, 陈冉, 冯颖, 姜宁. 调节性B细胞调控寄生虫感染的研究进展[J]. 中国畜牧兽医, 2024, 51(2): 790-798.
[3]	李宁, 蒋昀轩, 刘德凤, 闫国宁, 申明月, 张树宇, 黄博涵, 贾宝玉, 徐力, 魏凯. 两种动物胆汁酸对热应激小鼠肝脏和空肠保护效果的比较研究[J]. 中国畜牧兽医, 2023, 50(7): 2729-2739.
[4]	雷铭凯, 班斌, 殷炜琦, 张俊梅, 石攀峰, 王丽, 周扬, 张春香, 李碧波. 包被叶酸和包被牛磺酸对热应激种公羊精液品质、血清和精浆抗氧化能力及激素水平的影响[J]. 中国畜牧兽医, 2023, 50(7): 2755-2765.
[5]	李媛媛, 毛妍, 梁曾恩妮, 杨成林, 邬静, 袁志航, 袁莉芸. 紫花地丁药理作用研究进展[J]. 中国畜牧兽医, 2023, 50(7): 2998-3006.
[6]	陆辉, 蔡高峰, 武彩红, 邱树磊, 袁橙, 陈晓兰, 刘芸, 覃凯莉. 茶树菇多糖对小鼠脾脏淋巴细胞免疫调节的影响[J]. 中国畜牧兽医, 2023, 50(5): 1828-1835.
[7]	姚家威, 黄钰洁, 陈志胜, 王丙云, 张晖. α-酮戊二酸二甲酯预处理对犬脂肪源间充质干细胞免疫调节能力的影响[J]. 中国畜牧兽医, 2023, 50(4): 1642-1652.
[8]	陈少俊, 李刚, 奈子达, 刘娣, 姜新鹏. 基因编辑益生菌在动物肠道健康上的研究及应用[J]. 中国畜牧兽医, 2023, 50(3): 1016-1024.
[9]	徐倩倩, 杨万群, 王玉波, 吴菲, 代娟娟, 秦蜜蜜, 庄金秋, 王艳, 沈志强, 李继昌. 熟地黄多糖对环磷酰胺诱导的免疫抑制小鼠的免疫调节作用研究[J]. 中国畜牧兽医, 2023, 50(2): 693-703.
[10]	雷小文, 刘珍妮, 孔智伟, 谭东海, 成笛, 张强, 李建军, 连海, 郭海宁, 钟云平. 蚯蚓液对高温环境下番鸭生长性能、血清热应激指标、激素水平及肠道功能的影响[J]. 中国畜牧兽医, 2023, 50(12): 4826-4836.
[11]	蔡燕子, 袁子文, 张兴平, 张丰裕, 祝亚梅, 史艳, 姜立冬, 华永丽, 魏彦明. 基于网络药理学探究党参-甘草提取物增强断奶仔猪免疫功能的作用机制[J]. 中国畜牧兽医, 2023, 50(10): 4279-4291.
[12]	武亚南, 石玉祥, 张永英, 刘冠慧, 宋伟光, 钟翠红, 王永霞. 活化Nrf2缓解热应激致肉鸡心肌细胞氧化损伤的研究[J]. 中国畜牧兽医, 2022, 49(9): 3343-3352.
[13]	高嘉豪, 乔彦杰, 连科迅, 王蒙蒙, 谷新利, 邵永斌. 甘草酸对脂多糖应激黄羽肉仔鸡免疫性能的影响[J]. 中国畜牧兽医, 2022, 49(9): 3419-3427.
[14]	刘锐, 李江凌, 杜华锐, 杨朝武, 陈家磊, 赵素君, 王秋实. 鸡胸腺组织中免疫相关circRNA鉴定[J]. 中国畜牧兽医, 2022, 49(6): 2022-2032.
[15]	严啊妮, 沈奔, 王洪荣, 冯春燕, 魏宗友. 过瘤胃葡萄糖和缓释尿素对热应激绵羊生产性能及瘤胃发酵功能的影响[J]. 中国畜牧兽医, 2022, 49(6): 2105-2115.