云南高峰牛MHC-DQB基因外显子2遗传多态性分析

›› 2011, Vol. 38 ›› Issue (10): 117-121.

云南高峰牛MHC-DQB基因外显子2遗传多态性分析

禹文海¹, 鲁绍雄², 和占龙¹, 刘红文³, 陆润峰⁴, 金建斌⁴

1. 中国医学科学院,北京协和医学院医学生物学研究所,云南昆明 650118;2. 云南农业大学动物科学技术学院,云南昆明 650201;3. 云南省家畜改良站,云南昆明 650224;4. 云南省广南县畜牧局,云南广南 663300

收稿日期:2011-03-20 修回日期:1900-01-01 出版日期:2011-10-20 发布日期:2011-10-20
通讯作者: 鲁绍雄

Genetic Polymorphism of the Exon 2 of MHC-DQB Gene in Yunnan Humped Cattle

YU Wen-hai¹, LU Shao-xiong², HE Zhan-long¹, LIU Hong-wen³, LU Run-feng⁴, JIN Jian-bin⁴

1. Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China;2. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;3. Domestic Animal Improving Station of Yunnan Province, Kunming 650224, China;4. Bureau of Animal Husbandry, Guangnan County of Yunnan Province, Guangnan 663300, China

Received:2011-03-20 Revised:1900-01-01 Online:2011-10-20 Published:2011-10-20

摘要/Abstract

摘要： 本研究采用PCR直接测序和PCR-RFLP法研究了云南高峰牛MHC-DQB基因外显子2(DQB.2)的遗传多态性,并利用DNAMAN软件分析了云南高峰牛与部分物种DQB.2相应核苷酸序列的同源性。结果发现,共检测到了17个等位基因,其中Hae Ⅲ酶切位点存在10种基因型,由A、B、C、D和E 5个复等位基因控制;RsaⅠ酶切位点存在22种基因型,由A、B、C、D、E、F、G、H、I、J和K共11个复等位基因控制;TaqⅠ酶切位点只出现了1种基因型。分析发现,云南高峰牛DQB.2基因的12、25、63、96、106、126、152、156、165、204位的碱基表现出了多态性。所分析的物种该基因片段大小相同均为270 bp,云南高峰牛第224位碱基缺失及236位碱基插入现象。云南高峰牛与人、猪、马、绵羊、黄牛×瘤牛的核苷酸序列的同源性分别为81.4%、83.3%、78.1%、87.7%、86.6%。经χ²检验结果表明,Hae Ⅲ和TaqⅠ酶切位点处于Hardy-Weinberg平衡状态(P＞0.05),而RsaⅠ酶切位点则未达到Hardy-Weinberg平衡状态(P＜0.05)。

关键词: 云南高峰牛; 主要组织相容性复合体(MHC); DQB基因; 外显子2; 遗传多态性

Abstract: In this paper, the genetic polymorphism of the exon 2 of MHC-DQB gene was investigated by directly sequencing and PCR-RFLP in Yunnan humped cattle. Homology of the nucleotide acid sequences was analysis by DNAMAN software. The results showed that 17 alleles were found in this experiment,10 kinds of genotypes and 5 (A, B, C, D, E) alleles were found with enzyme Hae Ⅲ;22 kinds of genotypes and 11 (A, B, C, D, E, F, G, H, I, J, K) alleles were found with enzyme RsaⅠ;only one kind of genotype were found with enzyme TaqⅠ. Polymorphic sites were detected at base position 12, 25, 63, 96, 106, 126, 152, 156, 165 and 204. The results showed that there were same lengths (270 bp) of gene fragment in various species, but there was a nucleotide missing at position 224 and a nucleotide insertion at position 236 in Yunnan humped cattle. The homology between Yunnan humped cattle and human, swine, horse, sheep, Yellow cattle × Zebuand were 81.4%, 83.3%, 78.1%, 87.7% and 86.6%, respectively. The results of χ² test showed that the polymorphic loci of Hae Ⅲ and TaqⅠwere in Hardy-Weinberg equilibrium (P＞0.05), but the locus of RsaⅠ was not in equilibrium (P＜0.05).

Key words: Yunnan humped cattle; MHC; DQB gene; exon 2; genetic polymorphism

中图分类号:

S813.8

禹文海;鲁绍雄;和占龙;刘红文;陆润峰;金建斌. 云南高峰牛MHC-DQB基因外显子2遗传多态性分析[J]. , 2011, 38(10): 117-121.

YU Wen-hai;LU Shao-xiong;HE Zhan-long;LIU Hong-wen;LU Run-feng;JIN Jian-bin. Genetic Polymorphism of the Exon 2 of MHC-DQB Gene in Yunnan Humped Cattle[J]. , 2011, 38(10): 117-121.

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