荧光标记微卫星分析广西树鼩群体的遗传多态性

doi:10.16431/j.cnki.1671-7236.2016.01.027

›› 2016, Vol. 43 ›› Issue (1): 182-190.doi: 10.16431/j.cnki.1671-7236.2016.01.027

荧光标记微卫星分析广西树鼩群体的遗传多态性

杨香娣^1,2, 曹骥¹, 杨春¹, 李瑗¹, 周玲丽^1,2, 罗旺^1,2, 王谷洋^1,2, 李科志¹

1. 广西壮族自治区肿瘤防治研究所, 实验研究部, 南宁 530021;
2. 广西医科大学研究生学院, 南宁 530021

收稿日期:2015-07-06 出版日期:2016-01-20 发布日期:2016-01-23
通讯作者: 曹骥 E-mail:caojicn@163.com
作者简介:杨香娣(1989-),女,湖南郴州人,硕士生,研究方向:丙型肝炎树鼩模型的研究及普通级广西树鼩种群的建立,E-mail:yxd323612@163.com
基金资助:
国家自然科学基金项目(81260080);广西自然科学基金项目(2013GXNSFAA019210);2015年广西研究生教育创新计划资助项目(YCSZ2015114)

The Genetic Polymorphism Analysis in Tree Shrew(T.belangeri chinensis) based on Fluorescent Microsatellite

YANG Xiang-di^1,2, CAO Ji¹, YANG Chun¹, LI Yuan¹, ZHOU Ling-li^1,2, LUO Wang^1,2, WANG Gu-yang^1,2, LI Ke-zhi¹

1. Department of Experimental Research, Guangxi Cancer Institute of Guangxi Zhuang Autonomous Region, Nanning 530021, China;
2. Graoluate School. Guangxi Medical University, Nanning 530021, China

Received:2015-07-06 Online:2016-01-20 Published:2016-01-23

摘要/Abstract

摘要： 选取10个微卫星位点,结合荧光标记(FAM) PCR扩增技术,运用毛细管电泳检测扩增产物,利用Microsatellite Toolkit、PopGene软件计算遗传相关指标,探讨广西树鼩群体的遗传多态性。结果发现,在10个微卫星基因座中,共检测到64个等位基因,平均等位基因数为6.4个,平均有效等位基因数为3.7892,平均观察杂合度和平均期望杂合度为0.6156和0.6963,平均多态信息含量为0.6367,其中7个是高度多态位点,3个属中度多态位点。此外,在10个微卫星基因座中,有5个位点偏离Hardy-Weinberg平衡(P<0.01)。结果表明广西树鼩遗传多态性较丰富,所选微卫星位点基本可用于评估广西树鼩群体遗传多样性。

关键词: 广西树鼩; 毛细管电泳; 微卫星DNA; 遗传多态性

Abstract: A total of 10 fluorescent labeling microsatellite markers detected by capillary electrophoresis were selected to analyze the genetic diversity of tree shrew(T.belangeri chinensis).Microsatellite Toolkit and PopGene softwares were used to calculate the genetic correlation indexes to assess the genetic diversity of the population.64 alleles with 6.4 alleles per locus were identified among 10 microsatellite loci.The average effective number of alleles, observed heterozygosity, expected heterozygosity and polymorphic information content were 3.7892, 0.6156, 0.6963, and 0.6367, respectively.Seven of all loci were high polymorphic, and three were moderate polymorphic.Five loci extremely significantly deviated from Hardy-Weinberg equilibrium(P<0.01).These novel polymorphic microsatellite markers indicated high genetic diversity, which will be used to evaluate genetic diversity in tree shrew.

Key words: tree shrew(T.belangeri chinensis); capillary electrophoresis; microsatellite DNA; genetic polymorphism

中图分类号:

S813.3

杨香娣, 曹骥, 杨春, 李瑗, 周玲丽, 罗旺, 王谷洋, 李科志. 荧光标记微卫星分析广西树鼩群体的遗传多态性[J]. , 2016, 43(1): 182-190.

YANG Xiang-di, CAO Ji, YANG Chun, LI Yuan, ZHOU Ling-li, LUO Wang, WANG Gu-yang, LI Ke-zhi. The Genetic Polymorphism Analysis in Tree Shrew(T.belangeri chinensis) based on Fluorescent Microsatellite[J]. , 2016, 43(1): 182-190.

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荧光标记微卫星分析广西树鼩群体的遗传多态性

The Genetic Polymorphism Analysis in Tree Shrew(T.belangeri chinensis) based on Fluorescent Microsatellite

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