从江香猪抑制素α基因克隆及其卵巢表达研究

doi:10.16431/j.cnki.1671-7236.2016.01.028

›› 2016, Vol. 43 ›› Issue (1): 191-196.doi: 10.16431/j.cnki.1671-7236.2016.01.028

从江香猪抑制素α基因克隆及其卵巢表达研究

张笑^1,2, 苏艳^1,2, 杨世彬^1,2, 王嘉福^1,3, 冉雪琴³

1. 贵州大学农业生物工程研究院, 贵阳 550025;
2. 贵州大学生命科学学院, 贵阳 550025;
3. 贵州大学动物科学学院, 贵阳 550025

收稿日期:2015-08-10 出版日期:2016-01-20 发布日期:2016-01-23
通讯作者: 王嘉福 E-mail:jfwang@gzu.edu.cn
作者简介:张笑(1993-),女,河北邯郸人,学士,研究方向:生物技术,E-mail:xzhang07@163.com
基金资助:
国家高技术研究发展计划(863计划)课题(2013AA102503);贵州省农业科技攻关项目(黔科合NY[2013]3073号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州大学"大学生创新训练计划"项目(201410657014);"基础兽医学教学团队"(BKJX2012007)

Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig

ZHANG Xiao^1,2, SU Yan^1,2, YANG Shi-bin^1,2, WANG Jia-fu^1,3, RAN Xue-qin³

1. Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China;
2. College of Life Sciences, Guizhou University, Guiyang 550025, China;
3. College of Animal Science, Guizhou University, Guiyang 550025, China

Received:2015-08-10 Online:2016-01-20 Published:2016-01-23

摘要/Abstract

摘要： 为了探索抑制素α基因(inhibin-α,INHA)与从江香猪繁殖性状之间的相关性,试验采用特异性聚合酶链式反应(PCR)技术克隆从江香猪INHA基因,测定其核苷酸序列,通过等位基因特异性PCR(allele-specific PCR,AS-PCR)方法检测从江香猪低产群与高产群之间INHA基因的多态性变化,以实时荧光定量PCR技术检测高产、低产从江香猪卵巢组织中INHA基因的表达量。结果表明,从从江香猪基因组中成功克隆了INHA基因,编码区完整,全长1095 bp,编码364个氨基酸;经比对发现, INHA基因外显子2序列中存在2个候选SNPs位点(G359A和A373G)。经大样本检测,从江香猪高产群与低产群之间2个候选SNPs位点的基因频率没有明显差异;相比之下,高产从江香猪卵巢中INHA基因的表达量较高。研究结果提示,从江香猪INHA基因结构保守,可能主要通过基因的表达量变化调节从江香猪卵巢的生长和卵泡的发育。

关键词: 从江香猪; 抑制素; 基因克隆; 卵巢; SNP

Abstract: To reveal the relationship between inhibin-α(INHA) gene and the reproductive traits of Congjiang Xiang pig, INHA gene was cloned and sequenced taking the genomic DNA of Congjiang Xiang pigs as templates by polymerase chain reaction(PCR) method.The polymorphisms of INHA gene were tested in Congjiang Xiang pig populations with high-litter size and low-litter size using allele-specific PCR(AS-PCR) method.The expression profile of INHA gene in ovaries was detected from Congjiang Xiang pigs with high-litter yiled or low-litter yiled by Real-time PCR method.The complete coding region of INHA gene was 1095 bp in length, which coded for 364 amino acid residues.Compared with the known sequence, two candidate sites, G359A and A373G, were found out from exon 2 region of INHA gene in Congjiang Xiang pig.After investigation for the two sites in a large population, the frequency of alleles between two populations was not significant and without obvious relativity with the litter yiled of Congjiang Xiang pig.However, the INHA mRNA level in the ovary of Congjiang Xiang pig with high-litter yiled was higher than that with low-litter yiled.It suggested that INHA gene was much conserved, INHA gene expression level might be concerned for the regulation of ovary growth and follicle development in Congjiang Xiang pig breed.

Key words: Congjiang Xiang pig; inhibin; gene cloning; ovary; SNP

中图分类号:

张笑, 苏艳, 杨世彬, 王嘉福, 冉雪琴. 从江香猪抑制素α基因克隆及其卵巢表达研究[J]. , 2016, 43(1): 191-196.

ZHANG Xiao, SU Yan, YANG Shi-bin, WANG Jia-fu, RAN Xue-qin. Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig[J]. , 2016, 43(1): 191-196.

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从江香猪抑制素α基因克隆及其卵巢表达研究

Cloning of Inhibin-α Gene and its mRNA Expression Pattern in the Ovary of Congjiang Xiang Pig

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